7 results about "Bactericidin" patented technology

This invention belongs to the field of biopesticide technology, specifically relating to a biopesticide for controlling citrus thrips. The biopesticide, used to control citrus thrips, comprises an active ingredient derived from a compound of *Bretschneidera sinensis* powder and methyl salicylate, or a compound of *Bretschneidera sinensis* and ethyl spinosad; wherein the effective bacterial count of the *Bretschneidera sinensis* powder is 2.5 × 10⁻⁶. 8 Spores / g. This invention discovered that *Cladosporium flavum* is effective in killing citrus thrips and can be used as a microbial agent for the control of citrus thrips. Furthermore, this invention combines *Cladosporium flavum* with other existing bioactive ingredients and found that when combined with methyl salicylate or spinosad, it has a synergistic effect on killing citrus thrips. Compared with single-ingredient formulations, it can improve the control effect on citrus thrips, possessing the advantages of being green, safe, and highly efficient, and can provide support for the research and development of biological pesticides for the control of citrus thrips.

The present application belongs to the technical field of biological pharmacy, and particularly relates to a method for extracting and purifying spinosad. The method for extracting and purifying spinosad from saccharopolyspora spinosa fermentation broth by combining enzymolysis and acid extraction reduces the amount of organic solvent; the membrane separation technology is used to purify the spinosad extract, the microfiltration and ultrafiltration are used to remove the bacterial bodies, insoluble proteins, colloid, pigments and other macromolecular impurities, and the nanofiltration is used to remove small molecular water-soluble impurities, so as to realize the purification of the extract; the two-phase extraction is used to realize the second purification of the spinosad; and the evaporation crystallization is used to realize the third purification of the spinosad. Compared with the prior art, the method for extracting and purifying spinosad has the advantages of simple process, high efficiency, environmental protection, low consumption, and the like.

The invention discloses a fermentation type high astaxanthin microalgae compound for aquaculture and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the fermentation type high astaxanthin microalgae compound comprises a fermentation product of haematococcus pluvialis subjected to stress induction culture, the haematococcus pluvialis is obtained by stress induction culture through a gradient nitrogen deficiency-pulse carbon supplementation method, and the haematococcus pluvialis is a high astaxanthin microalgae compound. The astaxanthin content in the dry weight is greater than or equal to 8.0 mg / g; the fermentation product is prepared by carrying out wall breaking on haematococcus pluvialis subjected to stress induction culture and then co-fermenting the haematococcus pluvialis and lactobacillus plantarum under an anaerobic condition; in the compound, the active titer of the phytocidin generated by the lactobacillus plantarum is greater than or equal to 600AU / g; after the compound is treated in simulated gastric juice with the pH value of 2.0 for 2 hours, the retention rate of astaxanthin is greater than or equal to 75%. The compound disclosed by the invention not only contains high-concentration astaxanthin, but also is rich in probiotic metabolites, so that the synergistic enhancement of oxidation resistance and immune regulation can be realized.

The invention discloses a spinosad culture medium optimization method and device based on machine learning, and belongs to the technical field of microbial fermentation. According to the method, deep learning and Bayesian optimization technologies are combined to optimize the ratio of the spinosad fermentation culture medium, firstly, the characteristics of the culture medium are learned through a deep neural network, and then model parameters are adjusted by using a Bayesian optimization algorithm, so that intelligent control and optimization of the ratio of the microbial culture medium are realized; the microorganism production efficiency and the product quality are improved. According to the method, technical support is provided for the fields of medicine production, food processing, environmental protection and the like through intelligent analysis and accurate prediction, and the method has remarkable market prospects and application value.

A preparation method for Saccharopolyspora spinosa Spn016 and spinosad, and a screening method for Saccharopolyspora spinosa. The described Saccharopolyspora spinosa Spn016 has a classification name of Saccharopolyspora spinosa and was deposited in the China General Microbiological Culture Collection Center in Beijing, China under CGMCC accession No. 30471 on April 28, 2024. The present invention can solve the problem of Saccharopolyspora spinosa growing slowly in the prior art and pertains to the field of Saccharopolyspora spinosa.

The invention belongs to the technical field of strain mutagenesis, and particularly relates to a construction method and application of spinosad J and L producing strains. The strain is Saccharopolyspora spinosa AND8695, and is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC No.36070, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. A specific mutagenesis mode combination (ARTP / NTG compound mutagenesis) is adopted for the SP101 strain, the AND8695 strain with excellent properties is obtained, the shake flask production level of the AND8695 strain is equivalent to that of the original strain SP101, and the strain can be used for producing spinosad J and L through a specific culture mode.

The invention provides an application of PKW14684.1 as a negative regulatory factor in improving the spinosad production capacity of saccharopolyspora spinosa. The invention also provides an engineering modified bacterium for high yield of spinosad. The engineering modified bacterium does not express PKW14684.1, or the expressed PKW14684.1 does not have activity or the activity is reduced. The invention also provides a method for improving the yield of spinosad produced by saccharopolyspora spinosa. The method comprises the following steps: inhibiting the activity of PKW14684.1, further inhibiting the activity of a regulatory factor by adding salicylic acid and the like into a culture medium, reducing the negative regulation effect of the regulatory factor on a spinosad synthetic gene, and improving the yield of spinosad. It is found for the first time that PKW14684.1 is a negative regulation factor for synthesis of spinosad, the regulation effect is obvious, the yield of spinosad can be effectively increased by inhibiting the transcription factor, and the application has important significance on development of the industrial microorganism industry.