2results about How to "Fast swelling" patented technology

This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.

The application belongs to the technical field of biology and provides a preparation method and application of fish type I collagen-based self-healing composite hydrogel, which comprises the following steps: taking fish processing by-products as raw materials, using enzyme extraction and salt-out column chromatography purification integrated technology to prepare fish type I collagen; taking chitosan as raw material to perform oxidation, separating and purifying the sample after oxidation to obtain dialdehyde chitosan; on the basis, taking fish type I collagen as a base material, taking genipin as a crosslinking agent, adding chitosan and dialdehyde chitosan, performing Schiff base reaction, and obtaining fish type I collagen-based self-healing composite hydrogel. The hydrogel maintains the triple helix structure of fish type I collagen, has good biocompatibility, good self-healing performance, good injection performance, good thermal stability, good effects of promoting frostbite tissue detumescence and promoting wound healing, and can be applied in the field of wound dressings.