A kind of bacillus amyloliquefaciens wh3 and its preparation method and application

A technology for dissolving amyloid spores and bacilli, applied in the field of immunology, can solve problems such as toxic and side effects, and achieve the effects of easy preparation, high biological safety, and good immune enhancement effect.
CN102286408AInactive Publication Date: 2011-12-21武汉光谷世傲生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
武汉光谷世傲生物科技有限公司
Publication Date
2011-12-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Bacillus amyloliquefaciens WH3 strain, and a preparation method and application thereof. The preparation method comprises the following steps: 1, separation and identification of bacteria: separating bacteria resistant to rape sclerotinia rot from rape seedlings, and carrying out 16SrDNA and morphological identification to determine that the WH3 strain obtained by separation is Bacillus amyloliquefaciens; 2, separation, purification and identification of an antifungal active substance Safenour: fermenting the WH3 strain, extracting the antifungal active substance, separating and purifying through a sephadex column, and carrying out MALDI-TOF (matrix-assisted laser desorption / ionization-time of flight ) mass spectrometry on the active antifungal substance to inferthat the substance is a ring type polypeptide; and 3, application of the strain in the preparation of vaccines and immunological adjuvants. After the Safenour used as the adjuvant is mixed with a protein antigen and mice are respectively immunized through oral administration and injection of the mixture, effective body fluid and cellular immune response can be activated, and a high-titer specificantibody can be detected in the blood serum. The Safenour has low production cost and high stability, does not need to be emulsified when mixed with the antigen, and has a better immunoenhancement effect in comparison with Freund adjuvants and cholera toxin B subunits.
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Description

technical field

[0001] The present invention belongs to the field of immunology, and more specifically relates to a Bacillus amyloliquefaciens WH3 strain, and also relates to a preparation method of a Bacillus amyloliquefaciens WH3 strain, and also relates to a method for preparing an immunization of a Bacillus amyloliquefaciens WH3 strain The application in adjuvants is also applicable to cyclic peptides with similar structures produced by other microorganisms. Background technique

[0002] Immune adjuvants refer to substances that can enhance the immunogenicity of antigenic substances, enhance the immune response ability of the body against antigens, or change the type of immune response, but have no antigenicity when applied simultaneously or in advance with antigens.

[0003] The mechanism of action of adjuvants is extremely complex, and the current research believes that they mainly include: (1) Antigen storage, mineral colloids, latex adjuvants, etc. can form an antige...

Claims

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