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Bacillus licheniformis strain, application thereof and method for producing poly-gamma-glutamic acid thereby

A technology based on Bacillus licheniformis and glutamic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high production costs, achieve low production costs, simple genetic operations, and easy product separation and extraction Effect

Inactive Publication Date: 2010-11-10
HEBEI WELCOME PHARMACEUTICAL CO LTD +1
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Problems solved by technology

Although the above method has improved the yield of poly-γ-glutamic acid, it needs to add high-concentration glycerol in the fermentation process, so its production cost is high

Method used

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  • Bacillus licheniformis strain, application thereof and method for producing poly-gamma-glutamic acid thereby
  • Bacillus licheniformis strain, application thereof and method for producing poly-gamma-glutamic acid thereby
  • Bacillus licheniformis strain, application thereof and method for producing poly-gamma-glutamic acid thereby

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Abstract

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

Description

A kind of bacillus licheniformis strain and its application and method for producing poly-γ-glutamic acid technical field The present invention relates to bacillus and its use, in particular to the bacillus licheniformis strain and its use and its production method for poly-γ-glutamic acid. Background technique Bacillus licheniformis is a kind of facultative anaerobic saprophytic bacteria widely existing in nature. In industry, it is often used as the production bacteria of proteolytic enzymes, amylases, bioactive agents, antibiotics, etc. At present, researchers have used specific Bacillus licheniformis to produce poly-γ-glutamic acid, such as someone using Bacillus licheniformi A35 to produce poly-γ-glutamic acid (see reference 1 for details: ChengC, AsadaY, AaidaT. Agric Biol Chem, 1989, 53:2369-2375;). However, the use of the bacterial strain to produce poly-γ-glutamic acid has the disadvantages of long fermentation period and low fermentation yield. In order to sol...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/10
Inventor 陈守文谢萍魏雪团刘晓雷冀志霞郭威张红艳孙君伟李海涛李建波米造吉黄品奇王正品
Owner HEBEI WELCOME PHARMACEUTICAL CO LTD
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