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Preparation method trans hepatitis B surface antigen (HBsAg) gene salt algae

A hepatitis B, surface antigen technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve the problems of inability to process eukaryotic proteins, huge equipment investment, pollution of animal viruses, etc. The effect of easy industrial production, no seasonal restrictions, and simple culture conditions

Inactive Publication Date: 2003-04-16
薛乐勋
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AI Technical Summary

Problems solved by technology

Compared with production systems such as microbial fermentation and transgenic animals, it has many advantages: 1. Some microbial systems cannot perform accurate post-translational processing and protein glycosylation of eukaryotic proteins; E. coli fermentation often produces some insoluble polymers, It is extremely difficult to redissolve and fold these polymers into natural proteins; in addition, fermentation often requires huge investment in equipment
2. The cost of animal cell cultivation is expensive, and the use of transgenic animals to produce recombinant proteins may contaminate animal viruses, which may pose a potential threat to humans

Method used

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  • Preparation method trans hepatitis B surface antigen (HBsAg) gene salt algae
  • Preparation method trans hepatitis B surface antigen (HBsAg) gene salt algae
  • Preparation method trans hepatitis B surface antigen (HBsAg) gene salt algae

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Experimental program
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Embodiment

[0022] 1. Salina culture technology

[0023] 1. Liquid culture: the algae were inoculated in Mclachlan culture medium, and the culture conditions were as follows: temperature 25±2°C, light intensity 3000 lux, light culture for 14 hours a day, and dark culture for 10 hours.

[0024] 2. Solid culture: Add agar concentration of 0.5-0.8% to the Mclachlan culture medium, use sterilized white gold fungus to inoculate the algae from the liquid culture medium on the surface of the solid medium immediately on the ultra-clean bench, put it in the incubator and cultivate it in the incubator Fluorescent tubes need to be configured for irradiation in the medium, and the culture is carried out according to the requirements of liquid culture conditions.

[0025] 2. Construction of HBsAg Salina expression plasmid pCAMBIA-CtxB-SS1

[0026] 1. Construction of recombinant hepatitis B surface antigen SS1 fusion gene

[0027] The sequences of hepatitis B virus S gene, Pre-S2 gene and Pre-S1 gene...

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Abstract

A transferred hepatitis B surface antigen (HBsAg) gene halophila is prepared by recombining the exogenous gene HBsAg into the cells of eucaryotic monocell halophila, so obtaining the transgenic halophila used to prepare the oral vaccine is hepatitis B. Its advantages are high reproduction speed, simple culture condition, low cost, and rich nutrients contained by halophila cells.

Description

(1) Field of invention: [0001] The invention relates to a method for preparing Salina salina transgenic for hepatitis B surface antigen (HBsAg) by using genetic engineering technology. (2) Background of the invention [0002] Hepatitis B virus infection is an infectious disease that seriously endangers human health. Chronic hepatitis B can easily develop into liver cirrhosis and liver cancer. The infection rate of hepatitis B is very high, and it is estimated that about 350 million people in the world are chronic carriers of hepatitis B virus. my country is a high infection area of ​​hepatitis B, the infection rate of hepatitis B virus is 57.68%, and the chronic carrier rate of hepatitis B virus is 9.75%. The main means of controlling hepatitis B infection is vaccination. The vaccine currently used clinically is mainly composed of HBsAg protein particles expressed by Saccharomyces cerevisiae. The effect is good, and it has played a huge role in controlling the spread of he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/29C12N1/13C12N15/51C12N15/62C12N15/79
Inventor 薛乐勋潘卫东姜国忠陈占宽严海燕王建民吕玉民张贵星谢华郭玉忠牛向丽侯桂琴李杰王建人杜保华
Owner 薛乐勋
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