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Method for performing outdoor culture on isochrysis galbana parke suitable for southern climatic conditions

A technology of isochrysis algae and conditions, which is applied in the field of aquaculture and achieves the effects of simple method, simple and easy method, and simple cultivating tool.

Active Publication Date: 2017-12-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems existing in the large-scale cultivation of dinoflagellates in southern China, and to provide a method for outdoor cultivation of dinoflagellates suitable for southern climate conditions, using outdoor large white barrels to seal and inflate the culture , the method is simple and easy to implement, has low requirements for cultivation conditions, low cultivation cost and is suitable for outdoor large-scale cultivation in southern China climate conditions

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] Culture equipment: the culture equipment includes a white plastic bucket, a transparent plastic cover covering the mouth of the white plastic bucket, a trachea, an air stone and an air pump, and the trachea and the air stone are boiled and sterilized.

[0031] In early June 2017, at the Zhanjiang Southeast Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, 8 white plastic barrels with a volume of 200 liters were used to cultivate Isochrysis globosa (high temperature resistant 3011 strain). Rinse the white plastic bucket and the transparent plastic cover on the mouth of the bucket with fresh water and disinfect it with a high-concentration sodium hypochlorite solution, that is, add 70 ml of sodium hypochlorite solution (active chlorine content ≥ 7.5wt%) to each bucket and fill it up with 25‰ seawater, seal and soak overnight. Discharge 30L of sterilized sea water the next morning, put the air stone in the sterilized sea wate...

Embodiment 2

[0033] Culture equipment: the culture equipment includes a white plastic bucket, a transparent plastic cover covering the mouth of the white plastic bucket, a trachea, an air stone and an air pump device, and the trachea and the air stone are boiled and sterilized.

[0034]In late June 2017, at the Zhanjiang Southeast Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, 12 white plastic barrels with a volume of 100 liters were used to cultivate Isochrysis globosa (strain 3011 resistant to high temperature). Rinse the white plastic bucket and the transparent plastic cover on the mouth of the bucket with fresh water and disinfect it with a high-concentration sodium hypochlorite solution. Add 30 ml of sodium hypochlorite solution (active chlorine content ≥ 7.5wt%) to each bucket and fill up with seawater with a salinity of 25‰ , seal and soak overnight. Discharge 20L of sterilized sea water the next morning, put the air stone in the st...

Embodiment 3

[0036] Culture equipment: the culture equipment includes a white plastic bucket, a transparent plastic cover covering the mouth of the white plastic bucket, a trachea, an air stone and an air pump device, and the trachea and the air stone are boiled and sterilized.

[0037] In late June 2017, at the Zhanjiang Southeast Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, 12 white plastic barrels with a volume of 100 liters were used to cultivate Isochrysis globosa (strain 3011 resistant to high temperature). Rinse the white plastic bucket and the transparent plastic cover on the mouth of the bucket with fresh water and disinfect it with a high-concentration sodium hypochlorite solution. Add 40 ml of sodium hypochlorite solution (active chlorine content ≥ 7.5wt%) to each bucket and fill up with seawater with a salinity of 25‰ , seal and soak overnight. Discharge 20L of sterilized sea water the next morning, put the air stone in the s...

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PUM

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Abstract

The invention discloses a method for performing outdoor culture on isochrysis galbana parke suitable for southern climatic conditions. According to the method, an outdoor white plastic bucket is adopted for performing sealed aerated culture, and the culture appliance comprises the white plastic bucket, a transparent plastic cover covering the opening of the white plastic bucket, an air pipe, an air stone and a pumping device. The method is simple, feasible, low in requirement on culture conditions, low in culture cost and suitable for outdoor large-scale cultivation suitable for China southern climatic conditions, and the requirements of China southern marine economic shellfishes on important initial feed isochrysis galbana parke in breeding seasons are met.

Description

Technical field: [0001] The invention relates to the field of aquaculture, in particular to a method for outdoor culturing dinoflagellates suitable for southern climate conditions. Background technique: [0002] Isochrysis galbana (Isochrysis galbana) belongs to Chrysophyta, Chrysophyceae, Isochrysis, Isochrysis, Isochrysis genus, is a widely distributed marine planktonic unicellular algae, its cells are naked, Shapes vary, but most are oval. Under the optical microscope, the side view is oblong or rectangular, and the cells are 4.4-7.1 microns long, 2.7-4.4 microns wide, and 2.4-3 microns thick. It can move vigorously, making slow and steady rotation or swirling movement during movement, often in one direction, and the vibration of flagella can be seen when the movement is slow. Mainly asexual binary fission reproduction. Because of its small size, fast reproduction, rich nutrition, moderate movement speed, and no cellulose-containing cell wall, it is easy to be caught, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 马海涛喻子牛肖述张跃环
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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