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Pseudomonas fluorescens and its biological agent and its application in the control of sugarcane whip smut

A technology for Pseudomonas fluorescens, biological preparations, applied in the direction of chemicals for biological control, biocides, microorganism-based methods, etc., to achieve the effects of low requirements on culture conditions, strong inhibitory effect, environmentally friendly and non-toxic sources

Active Publication Date: 2017-05-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the screening of biocontrol bacteria for sugarcane whip smut, Liao Yongmei et al. (2004) conducted research, but only identified Pseudomonas

Method used

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  • Pseudomonas fluorescens and its biological agent and its application in the control of sugarcane whip smut
  • Pseudomonas fluorescens and its biological agent and its application in the control of sugarcane whip smut
  • Pseudomonas fluorescens and its biological agent and its application in the control of sugarcane whip smut

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: Isolation, purification and preservation of Pseudomonas fluorescens

[0027] (1) Configuration of LB medium: Configuration of LB medium: Weigh tryptone (Tryptone, Oxoid LTDLP0042, England) 10g, yeast extract (Yeast extract, Oxoid LTDLP0021, England) 5g, sodium chloride (NaCl, Sinopharm Chemical Reagent Co., Ltd., 10019318) 10g, add 1000mL of water and stir evenly, add 15g of agar, fully heat to dissolve, subpackage, sterilize at 121°C for 20min, and store for later use.

[0028] (2) Isolation and purification of Pseudomonas fluorescens:

[0029] Weigh 10g of the sugarcane rhizosphere soil sample from the sugarcane field of Yuejin Farm, South China Agricultural University, add it to a triangular flask filled with 90mL of sterile water, vibrate at 28°C and 200rpm for 1h, let it stand for 1-2h, absorb 1mL of soil to soak on the The supernatant was sequentially diluted to 10 with 0.9% normal saline by concentration gradient method. -6 Concentration, respecti...

Embodiment 2

[0035] Embodiment 2: The confrontation culture method measures the activity of Pseudomonas fluorescens

[0036] (1) The preparation of the LB medium is the same as in Example 1; the preparation of the LB liquid medium is except that agar is not added, and the other is the same as the formula of the LB solid medium, and the weighed reagent is fully dissolved and then distributed in conical flasks (per bottle of 100mL culture solution), stoppered and bandaged, sterilized at 121°C for 20min, cooled and stored for later use.

[0037] (2) Preparation of PDA medium: Weigh 200g of potatoes and cut into small pieces, add water and boil for 20-30min until it can be pierced by a glass rod, filter with eight layers of gauze; heat the filtrate, add 20g of glucose and 20g of agar powder, stir Evenly, after cooling for a while, add water to 1000mL, divide into Erlenmeyer flasks (100mL culture solution per bottle), stopper, bandage, sterilize at 121°C for 20min, cool and store for later use....

Embodiment 3

[0046] Embodiment 3: Pseudomonas fluorescens controls sugarcane whip smut

[0047] (1) The preparation of LB medium is the same as in Example 1. The preparation of LB culture fluid is the same as that in Example 2.

[0048] (2) The preparation of Pseudomonas fluorescens biological preparation is the same as that in Example 2.

[0049] (3) Inoculate by seed dressing method. 1) Streak and activate the biocontrol strain HN58 on the LB plate, pick a single colony after 1 to 2 days and culture it in LB liquid to OD at 28°C and 200rpm 600 It is about 1.5; 2) add 0.25g pathogenic bacteria smut fungus spore (see embodiment 2) in every 1kg soil, set and add three treatments of 5mL, 20mL, 100mL biocontrol bacteria culture solution respectively, each handle 3 repeat. For each repetition, 30 segments of sugarcane stem nodes are selected, so 4 kg of bacteria-carrying soil containing 1g of smut and 20mL, 80mL, and 400mL of biocontrol bacteria need to be prepared respectively; Also repe...

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Abstract

The invention belongs to the field of biopesticides, and discloses a strain of Pseudomonas fluorescens and its biological preparation and its application in preventing and treating whip smut of sugarcane. The strain of Pseudomonas fluorescens was named as Pseudomonas fluorescens HN58, and it was preserved in China Center for Type Culture Collection (CCTCC) on October 24, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC No: M2014511. The strain is isolated from sugarcane rhizosphere soil and can adapt to the local natural environment; it has a good inhibitory effect on sugarcane whip smut, and the source is environmentally friendly and non-toxic, and has little impact on the ecological environment; it has low requirements for culture conditions and has a good development and application prospects.

Description

technical field [0001] The invention belongs to the field of biological pesticides, in particular to a strain of Pseudomonas fluorescens and its biological preparation and its application in preventing and treating sugarcane whip smut. Background technique [0002] Sugarcane (Saccharum officinarum) is an important sugar crop in southern my country. More than 80% of sugar is made from sugarcane, which plays an important role in the national economy. Sugarcane is originally produced in tropical and subtropical regions with high temperature and humidity, which is very conducive to the occurrence and development of sugarcane diseases and insect pests. So far, more than 80 kinds of fungal diseases have been found in the world. In South China, sugarcane whip smut (sugarcane smut) is the most common and most serious fungal disease in production. Yield and quality have taken a serious toll. At present, the control of this disease in production is mainly controlled by chemical agen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/39
CPCA01N63/10C12N1/205C12R2001/39
Inventor 习平根邓华孙飞江立群孙霞袁玉花李敏慧古佳妍沈万宽姜子德
Owner SOUTH CHINA AGRI UNIV
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