Escherichia coli metabolism crude extract as well as preparation method and application of escherichia coli metabolism crude extract

Abstract

The invention discloses an escherichia coli metabolism crude extract as well as a preparation method and application of the escherichia coli metabolism crude extract. The preparation method comprises the following steps: fermenting escherichia coli GZ-34, and carrying out solid-liquid separation on an obtained fermented solution; adding ethyl acetate into obtained supernate for extraction, collecting an organic phase on an upper layer, carrying out rotary evaporation, dissolving the organic phase into methanol, and removing an undissolved substance by virtue of a filter membrane with a bore diameter of 0.22 micron, so as to obtain the escherichia coli metabolism crude extract. A biocontrol mechanism of the crude extract mainly shows up as inhibition of sexual cooperation of a haploid mycelium + and a haploid mycelium - of sugarcane smut bacteria, and a dicaryon mycelium with infective pathogenicity cannot be generated, so that the biocontrol effect is realized; meanwhile, the form growth of the two non-pathogenic hyphae of pathogenic bacteria are slightly influenced; and the biocontrol mechanism is of a great innovative significance. The escherichia coli metabolism crude extract is used for preventing and treating sugarcane diseases, and sugarcane and processing products of the sugarcane are relatively safely and reliably eaten.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and in particular relates to a crude metabolic extract of Escherichia coli and its preparation method and application. Background technique [0002] Sugarcane smut is a worldwide disease caused by smut (Sporisorium scitamineum Syd.), and it is also the most important fungal disease that endangers sugarcane production in my country. In recent years, it has generally occurred in major sugarcane producing areas such as Yunnan, Guangdong, and Guangxi in my country, and it is becoming more and more serious, especially in dry field sugarcane and rat root sugarcane fields. The incidence of disease-resistant varieties is less than 10%, and the incidence of susceptible varieties can be as high as more than 50%, or even as high as 80-90% in severe cases, causing huge economic losses. [0003] Ustilago cane whip is a semi-specific parasite, and the haploid thallus can grow on the artific...

AI Technical Summary

Problems solved by technology

The incidence of disease-resistant varieties is less than 10%, and the incidence of susceptible varieties can be as high as 50%, or even as high as 80-90% in severe cases, causing huge economic losses

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