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Escherichia coli metabolic crude extract and its preparation method and application

A technology for Escherichia coli and crude extract, which is applied in the field of Escherichia coli metabolic crude extract and its preparation, can solve problems such as economic loss, and achieve the effects of simple steps and simple operation.

Active Publication Date: 2020-11-13
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The incidence of disease-resistant varieties is less than 10%, and the incidence of susceptible varieties can be as high as 50%, or even as high as 80-90% in severe cases, causing huge economic losses

Method used

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  • Escherichia coli metabolic crude extract and its preparation method and application

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Embodiment 1

[0026] 1. Bacteria and culture medium

[0027] Escherichia coli GZ-34, whose deposit number is CCTCC NO: M2016353, was deposited at the China Type Culture Collection at Wuhan University, Wuhan, China on June 27, 2016. It has applied for CN in China 105969707A made public.

[0028] Ustilago cane, purchased from ATCC ( MYA-4638 TM ), WT17 and WT18 are separated + and – two haploid strains.

[0029] LB solid medium: tryptone 10g, yeast extract 5g, NaCl 10g, agar 15g, H 2 Dilute O to 1000ml, pH 7.0-7.2. Sterilize at 121°C for 20 minutes.

[0030] LB liquid medium: The only difference from LB solid medium is that it does not contain agar.

[0031] Yeps solid medium: yeast extract 10g, peptone 20g, sucrose 20g, agar 20g, H 2 Dilute O to 1000ml, pH 6.8-7.2. Sterilize at 121°C for 20 minutes.

[0032] Yeps liquid medium: Just do not add agar.

[0033] 2.1 Medium preparation

[0034] Prepare the medium as in step 1.

[0035] 2.2 Activation of Escherichia coli GZ-34 and activation of smut

[0036]...

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Abstract

The invention discloses a crude metabolic extract of Escherichia coli and its preparation method and application. In the present invention, Escherichia coli GZ‑34 is fermented, and the fermented liquid obtained is subjected to solid-liquid separation; ethyl acetate is added to the obtained supernatant for extraction, and the upper organic phase is collected and dissolved in methanol after rotary evaporation , Remove the insoluble matter with a filter membrane with a pore size of 0.22 μm to obtain a crude metabolic extract of Escherichia coli. The main biocontrol mechanism of the crude extract is to inhibit the sexual cooperation of the two haploid strains of the sugarcane whip smut + and –, because no dikaryotic mycelia with infection and pathogenicity can be formed, thereby achieving the goal of biological control. anti effect. Simultaneously less influence the morphological growth of two non-pathogenic haploid mycelia of pathogenic bacteria itself. This biological control mechanism has great innovation significance. Applying it to the prevention and control of sugarcane diseases will be safer and more reliable for the consumption of sugarcane and its processed products.

Description

Technical field [0001] The invention belongs to the field of plant disease prevention and treatment, and particularly relates to a crude extract of Escherichia coli metabolism and a preparation method and application thereof. Background technique [0002] Sugarcane smut is a worldwide disease caused by smut (Sporisorium scitamineum Syd.), and it is also the most important fungal disease that harms sugarcane production in my country. In recent years, it has generally occurred in major sugarcane producing areas such as Yunnan, Guangdong, and Guangxi in my country, and it is becoming more and more serious, especially in dry land and ratoon cane. The incidence of more resistant varieties is less than 10%, and the incidence of susceptible varieties can be as high as 50% or more, and even as high as 80-90% in severe cases, causing huge economic losses. [0003] Cane smut is a semi-obligate parasite, haploid cells can grow on artificial culture medium, but mycelium cannot survive for lon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04A01N63/20A01P3/00C12R1/19
CPCA01N63/10C12P1/04C12N1/205C12R2001/19
Inventor 邓音乐宋施豪付书娜孙秀云邓海飞叶佩仪
Owner SOUTH CHINA AGRI UNIV
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