17 results about "Escherichia albertii" patented technology

This utility model relates to the field of microbial detection technology, and in particular to an enrichment device for Shiga toxin-producing Escherichia coli. The technical solution includes an enrichment container body, a sealing cap at the top for sealing, a shock-absorbing pad at the bottom for reducing amplitude, and a stirring structure at the top of the sealing cap for mixing the sample solution. The stirring structure includes a motor for driving the mixture, and a rotating shaft fixedly mounted at the output end of the motor. The rotating shaft passes through the sealing cap and has multiple stirring blades spirally distributed on its exterior. This utility model, through the arrangement of the motor, stirring blades, and connecting rod, allows the motor to be activated after the sample solution and enrichment solution are added. The motor drives the rotating shaft, which in turn rotates the multiple stirring blades. The stirring blades stir the two liquids, enabling a more uniform mixing of the sample solution and enrichment solution, avoiding localized excessively high or low concentrations, and improving the uniformity and efficiency of the enrichment.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Z34B3 and application thereof. The amino acid sequence of the antibacterial peptide Z34B3 provided by the invention is shown as SEQ ID NO: 1, the secondary structure is an alpha helix, the molecular weight is small, and the antibacterial peptide Z34B3 can change the cell membrane permeability of various gram-negative bacteria including pasteurella multocida, escherichia coli and acinetobacter baumannii, inhibit the generation of cell membranes and play antibacterial and bactericidal roles. Moreover, the antibacterial peptide Z34B3 provided by the invention has low hemolytic activity and no cytotoxicity, and can be applied to preparation of products with bacteriostatic and bactericidal effects and preparation of drugs for preventing and / or treating bacterial infectious diseases. Furthermore, the C tail end of the antibacterial peptide Z34B3 is subjected to amidation modification, and compared with non-modification treatment, the stability can be improved.

The invention relates to Escherichia coli ML001, a specific bacteriophage thereof and an application of the Escherichia coli ML001 in preventing alcoholic liver diseases. The Escherichia coli strain ML001 and the specific bacteriophage thereof are preserved in China General Microbiological Culture Collection Center (CGMCC). Animal experiments prove that the bacteriophage can effectively reduce the level of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in the plasma of an alcoholic liver disease model mouse, which indicates that the bacteriophage has a definite liver protection function. The histopathologic examination further shows that after the phage is administered, the number of lipid droplets in the liver of the mouse is obviously reduced, and the liver lipid accumulation is effectively relieved. On the molecular mechanism level, the bacteriophage can down-regulate expression of fatty acid synthesis genes Srebf1 and Acly at the same time and inhibit a lipid synthesis pathway; meanwhile, the expression of a chemotactic factor Ccl6 is reduced, and the inflammatory response of the liver is relieved.

The utility model relates to sample sampling device technical field especially, it is used for the sampling device for shiga toxin producing escherichia coli detection. Its technical scheme includes sampling cylinder and rotates and installs the rotating sampling assembly in sampling cylinder, the bottom of rotating sampling assembly is provided with the lower cover of screw thread rotation and is installed in the bottom of sampling cylinder and is closed, rotating sampling assembly includes the outer sleeve rod and sets up in the inner sleeve rod of outer sleeve rod, the bottom end of inner sleeve rod is provided with the sampling clamp for shiga toxin producing escherichia coli sampling, the sampling clamp includes the fixed rod of rotation and is installed below sampling clamp, the bottom end fixedly connected with sampling spoon of fixed rod. The utility model is accurate in sampling, avoids to contaminate, realizes the accurate, safe, convenient sampling of all kinds of samples, improves the reliability and efficiency of shiga toxin producing escherichia coli detection, provides the powerful technical support for food safety, environmental protection and disease diagnosis etc.

The invention discloses a carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a detection kit and a kit using method, and through a large number of screening and verification experiments, a multiple primer group suitable for time-of-flight mass spectrometry detection is obtained. Therefore, the multi-PCR-time-of-flight mass spectrometry detection of the multi-drug-resistant genes of carbapenem-resistant enterobacteriaceae bacteria becomes possible. The detection kit prepared by adopting the primer group can be used for simultaneously detecting and identifying 10 common carbapenem drug-resistant genes, including klebsiella pneumoniae KPC gene, NDM gene, IMP gene, VIM gene and OXA gene, acinetobacter baumannii OXA gene, NDM gene, Escherichia coli OXA gene, IMP gene and serratia marcescens VIM gene. Meanwhile, the kit is high in sensitivity and specificity, dozens of times of detection can be carried out in the same batch, so that the detection efficiency is greatly improved, and the kit is suitable for rapid screening and genetic typing of carbapenem drug-resistant bacteria in clinical samples.

The present application relates to the technical field of microbial separation, and particularly relates to a separation method and application of intestinal probiotic escherichia; the separation method of intestinal probiotic escherichia is to take bovine large intestine content as raw material, to realize pre-screening of potential colonization strains through mucus anaerobic targeted enrichment, then to obtain resistant colonies through bile salt-short chain fatty acid stress primary screening, to scrape the colonies with metallic luster and fluorescence quenching ring through FITC-labeled mucus fluorescence indication secondary screening, and finally to obtain target strains through multiple PCR screening of negative virulence gene-positive functional gene, to realize directional separation of intestinal probiotic escherichia after purification of nutrient agar; the present application realizes targeted enrichment of intestinal colonization bacteria, environmental tolerance pressure screening and mucus binding visualization identification, solves the problems of strong randomness of traditional probiotic screening and inability to predict colonization potential, and provides a new strategy of efficient and safe probiotic strain screening for development of functional food and microecological preparation.

The invention is applicable to the technical field of microorganisms, and provides a lactobacillus paracasei Jlu-CH9 and application thereof, the lactobacillus paracasei Jlu-CH9 is preserved in China General Microbiological Culture Collection Center on January 4, 2026, the preservation number is CGMCC No.37281, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain Jlu-CH9 obtained by separation has certain tolerance to acid and cholate, has no hemolysis phenomenon, and is sensitive to various antibiotics; the compound has good inhibitory activity on dipeptidyl peptidase IV and has a certain potential of reducing blood sugar. The compound has relatively good inhibitory activity on a plurality of pathogenic bacteria including salmonella typhimurium, staphylococcus aureus, escherichia coli, clostridium perfringens and pseudomonas aeruginosa; the traditional Chinese medicine composition has good prevention and treatment effects on a DSS-induced mouse colitis model, and can repair intestinal barriers, regulate immune balance, remodel intestinal flora homeostasis and relieve colonic atrophy.

The invention relates to the technical field of biology, in particular to bacteroides fragilis FMBL Ba24006 DMX for metabolism of pectic polysaccharides and application, the bacteroides fragilis FMBL Ba24006 DMX is preserved in China Center for Type Culture Collection on January 20, 2026, the preservation number is CCTCC M 2026175, the bacteroides fragilis FMBL Ba24006 DMX has good pectin metabolism capacity, pectin can be effectively used as a carbon source to achieve rapid proliferation, and meanwhile, the bacteroides fragilis FMBL Ba24006 DMX can be used for metabolism of pectic polysaccharides. The bacterial strain has a good antibacterial effect on hemorrhagic Escherichia coli; the compound is sensitive and moderately sensitive to cefoxitin and rifampicin. The strain can produce a large amount of short-chain fatty acid when being used for fermenting pectin, and fermentation liquor taking the pectin as a carbon source has relatively strong scavenging capacity on DPPH and ABTS free radicals; a relatively good inhibition effect is achieved on the activity of alpha-amylase; the method can be used for preparing antioxidant products, fermented foods, health-care products, food additives and feeds, and has a wide application prospect.

The invention discloses Escherichia coli LNT I-2 and a method for stably generating lactose-N-tetrasaccharide on a large scale by using the Escherichia coli LNT I-2. The preservation number of the Escherichia coli LNT I-2 is CCTCC NO: M 20251742, the Escherichia coli LNT I-2 is classified and named as Escherichia coli LNT I-2, the preservation date is July 31, 2025, the Escherichia coli LNT I-2 can utilize a fermentation culture medium without glycerol or without glycerol as a supplementary material to stably ferment and produce LNT at high yield, lactose hydrolysate can be adopted as an inducer to induce synthesis of a product-lactose-N-tetrasaccharide, and the yield of the LNT is increased. The method meets the requirements of green and efficient synthesis of the LNT, and is beneficial to popularization of industrial large-scale microbial fermentation production of the LNT.

This invention relates to the field of biomedical technology, and particularly to a multidrug-resistant Escherichia coli bacteriophage and its applications. This invention has screened an Escherichia coli bacteriophage, vB_EOP_Se004, which exhibits good killing effects against clinically isolated multidrug-resistant Escherichia coli. It also demonstrates strong tolerance to temperature and pH, maintaining stable survival rates within a pH range of 4-10 and a temperature range of -20°C to 70°C. This bacteriophage can effectively address the resistance of multidrug-resistant Escherichia coli to multiple antibiotics, offering significant advantages in the clinical treatment of infections.

This invention relates to the field of biomedicine, specifically to an antibody for detecting Escherichia coli O45 and its application method. The antibody is a monoclonal antibody or its antigen-binding fragment, comprising the heavy chain CDR sequence shown in SEQ ID NO:1-3 and the light chain CDR sequence shown in SEQ ID NO:4-6. This invention also provides the nucleic acid molecule encoding the antibody, the expression vector, the host cell, and the preparation method thereof. Specificity identification results show that the antibody specifically binds to Escherichia coli O45 and shows no cross-reactivity with other Escherichia coli serotypes such as O157, O26, O103, O111, O121, and O145, as well as closely related serotypes of O55, and also shows no cross-reactivity with common foodborne pathogens. The indirect ELISA detection method based on this antibody has a detection limit of 1×10⁻⁶ for Escherichia coli O45. 2 The detection rate is CFU / mL, and the entire testing process can be completed within 24 hours. The monoclonal antibody provided by this invention can be used to prepare detection kits, test strips, and other products, and is suitable for rapid screening of O45 Escherichia coli in imported and exported food.

This invention relates to a fermenting strain of *Limosilactobacillus fermentum* LAF6 and its applications, belonging to the field of microbial technology. This strain has been deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC NO. 66595). Safety assessments show that the bacterium has no hemolytic activity, and drug sensitivity tests show that it is sensitive to a variety of common antibiotics. Furthermore, experiments show that the fermentation broth produced by this strain has good antibacterial effects against *Burkholderia cepacia*, *Escherichia coli*, *Propionibacterium acnes*, and *Candida albicans*, while also possessing excellent antioxidant and anti-inflammatory activities. Therefore, the strain has high safety, and its fermentation broth has excellent antibacterial properties, making it a mild and low-irritant raw material suitable for the preparation of daily chemical products, with broad application prospects and significant value for translational research.

The invention discloses an Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, a kit and a use method of the kit. Multiple PCR amplification primers and single-base extension primers are designed according to six virulence genes escV, stx2, hlyA, rfb, uidA and eaeA of Escherichia coli and six drug-resistant genes KPC, VIM, NDM, OXA, SHV and TEM. Wherein the nucleotide sequences of the upstream amplification primer and the downstream amplification primer of each gene are respectively shown as SEQ ID NO.1-SEQ ID NO.24, and the nucleotide sequences of the single base extension primer of each gene are respectively shown as SEQ ID NO.25-SEQ ID NO.36. According to the invention, a target product is obtained through a PCR amplification reaction; carrying out a dephosphorylation reaction and an extension reaction to obtain an extension product; and carrying out desalination treatment on the obtained extension product, carrying out sample application, and analyzing by using analysis software of a mass spectrometer. The kit has the characteristics of short period, high flux, high accuracy, better flexibility, easiness in implementation, high cost performance and the like, and provides a basis for clinical early detection of Escherichia coli infection.

The application discloses a cross primer nucleic acid isothermal amplification primer combination and method for detecting shiga toxin-producing escherichia coli. The primer combination is used for detecting 10 gene subtypes of stx1a, stx1c, stx1d, stx2a, stx2b, stx2c, stx2d, stx2e, stx2f and stx2g, and comprises primer group I with the nucleotide sequence of SEQ ID NO:1-5, primer group II with the nucleotide sequence of SEQ ID NO:6-10, and primer group III with the nucleotide sequence of SEQ ID NO:11-20. The method provided by the application is simple in operation, good in specificity, high in sensitivity, can complete nucleic acid detection within 50 min, provides a rapid screening result for a regulatory department to detect food pathogenic bacteria in major activity guarantee and emergency events. Meanwhile, the application firstly designs and screens the primer combination to realize detection of all gene subtypes of STEC, and in combination with a nucleic acid detection test strip and an integrated detection device, the whole process can be realized without instrument dependence and visual result judgment, and the requirement of on-site detection is greatly met.

This utility model relates to the field of microbial detection equipment technology, and in particular to a Shiga toxin-producing Escherichia coli enrichment culture device. The technical solution includes a culture tank body, with a sealing top cover on the top of the culture tank body. The neck of the culture tank body has two arc-shaped plates for fixation, and an alarm structure for temperature loss alarm is fixed to one side of the arc-shaped plate. This utility model utilizes a temperature sensor, a central control module, an electric push rod, and a buzzer. The temperature sensor transmits the real-time temperature to the central control module, which compares the real-time temperature with a preset temperature. If the temperature is higher or lower than the preset temperature, the electric push rod is activated to compress the guide plate, energizing the buzzer and emitting a buzzing sound. This allows relevant personnel to be immediately aware of abnormal temperature inside the culture tank and take timely measures to ensure the cultivation quality and efficiency of the equipment.

The present application relates to a bacillus velezensis (Bacillus velezensis) Bacillus velezensis ) B02 and its application, belonging to the field of microbial technology. The bacteria have been preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.66439. The strain is isolated, purified and screened from soil samples. Tests show that the bacteriostatic active substances produced by fermentation of the strain have good bacteriostatic effect on Burkholderia cenocepacia, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Pseudomonas putida, Enterococcus faecalis, Candida albicans and Aspergillus brasiliensis. Moreover, the strain has excellent heat resistance, cold resistance, light stability, pH stability and preservative effect, belongs to a mild low-stimulation raw material, and can be used for preparing daily chemical products. Therefore, the bacillus velezensis B02 and the bacteriostatic active substances produced by the bacillus velezensis B02 both have broad application prospect and important transformation research value.