7 results about "Escherichia albertii" patented technology

This utility model relates to the field of microbial detection technology, and in particular to an enrichment device for Shiga toxin-producing Escherichia coli. The technical solution includes an enrichment container body, a sealing cap at the top for sealing, a shock-absorbing pad at the bottom for reducing amplitude, and a stirring structure at the top of the sealing cap for mixing the sample solution. The stirring structure includes a motor for driving the mixture, and a rotating shaft fixedly mounted at the output end of the motor. The rotating shaft passes through the sealing cap and has multiple stirring blades spirally distributed on its exterior. This utility model, through the arrangement of the motor, stirring blades, and connecting rod, allows the motor to be activated after the sample solution and enrichment solution are added. The motor drives the rotating shaft, which in turn rotates the multiple stirring blades. The stirring blades stir the two liquids, enabling a more uniform mixing of the sample solution and enrichment solution, avoiding localized excessively high or low concentrations, and improving the uniformity and efficiency of the enrichment.

The utility model relates to sample sampling device technical field especially, it is used for the sampling device for shiga toxin producing escherichia coli detection. Its technical scheme includes sampling cylinder and rotates and installs the rotating sampling assembly in sampling cylinder, the bottom of rotating sampling assembly is provided with the lower cover of screw thread rotation and is installed in the bottom of sampling cylinder and is closed, rotating sampling assembly includes the outer sleeve rod and sets up in the inner sleeve rod of outer sleeve rod, the bottom end of inner sleeve rod is provided with the sampling clamp for shiga toxin producing escherichia coli sampling, the sampling clamp includes the fixed rod of rotation and is installed below sampling clamp, the bottom end fixedly connected with sampling spoon of fixed rod. The utility model is accurate in sampling, avoids to contaminate, realizes the accurate, safe, convenient sampling of all kinds of samples, improves the reliability and efficiency of shiga toxin producing escherichia coli detection, provides the powerful technical support for food safety, environmental protection and disease diagnosis etc.

The present application relates to the technical field of microbial separation, and particularly relates to a separation method and application of intestinal probiotic escherichia; the separation method of intestinal probiotic escherichia is to take bovine large intestine content as raw material, to realize pre-screening of potential colonization strains through mucus anaerobic targeted enrichment, then to obtain resistant colonies through bile salt-short chain fatty acid stress primary screening, to scrape the colonies with metallic luster and fluorescence quenching ring through FITC-labeled mucus fluorescence indication secondary screening, and finally to obtain target strains through multiple PCR screening of negative virulence gene-positive functional gene, to realize directional separation of intestinal probiotic escherichia after purification of nutrient agar; the present application realizes targeted enrichment of intestinal colonization bacteria, environmental tolerance pressure screening and mucus binding visualization identification, solves the problems of strong randomness of traditional probiotic screening and inability to predict colonization potential, and provides a new strategy of efficient and safe probiotic strain screening for development of functional food and microecological preparation.

This invention discloses a marine bacterium capable of preying on human pathogens and its applications, belonging to the field of microbial technology. The marine bacterium capable of preying on human pathogens is *Clostridium jejuae* (Jeju Island strain). Hahella chejuensis This strain (GXIMD14048, CGMCC No. 37275) was isolated from mangrove soil and exhibits predatory activity against Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, Escherichia coli, and Acinetobacter baumannii, with particularly significant predatory activity against Acinetobacter baumannii. This invention also provides a systematic screening method for this strain, including sample processing, isolation and purification, initial activity screening, and verification steps. This strain can be used to prepare antimicrobial agents and has promising application prospects.

This invention relates to the field of biomedical technology, and particularly to a multidrug-resistant Escherichia coli bacteriophage and its applications. This invention has screened an Escherichia coli bacteriophage, vB_EOP_Se004, which exhibits good killing effects against clinically isolated multidrug-resistant Escherichia coli. It also demonstrates strong tolerance to temperature and pH, maintaining stable survival rates within a pH range of 4-10 and a temperature range of -20°C to 70°C. This bacteriophage can effectively address the resistance of multidrug-resistant Escherichia coli to multiple antibiotics, offering significant advantages in the clinical treatment of infections.

This invention relates to the field of biomedicine, specifically to an antibody for detecting Escherichia coli O45 and its application method. The antibody is a monoclonal antibody or its antigen-binding fragment, comprising the heavy chain CDR sequence shown in SEQ ID NO:1-3 and the light chain CDR sequence shown in SEQ ID NO:4-6. This invention also provides the nucleic acid molecule encoding the antibody, the expression vector, the host cell, and the preparation method thereof. Specificity identification results show that the antibody specifically binds to Escherichia coli O45 and shows no cross-reactivity with other Escherichia coli serotypes such as O157, O26, O103, O111, O121, and O145, as well as closely related serotypes of O55, and also shows no cross-reactivity with common foodborne pathogens. The indirect ELISA detection method based on this antibody has a detection limit of 1×10⁻⁶ for Escherichia coli O45. 2 The detection rate is CFU / mL, and the entire testing process can be completed within 24 hours. The monoclonal antibody provided by this invention can be used to prepare detection kits, test strips, and other products, and is suitable for rapid screening of O45 Escherichia coli in imported and exported food.

This utility model relates to the field of microbial detection equipment technology, and in particular to a Shiga toxin-producing Escherichia coli enrichment culture device. The technical solution includes a culture tank body, with a sealing top cover on the top of the culture tank body. The neck of the culture tank body has two arc-shaped plates for fixation, and an alarm structure for temperature loss alarm is fixed to one side of the arc-shaped plate. This utility model utilizes a temperature sensor, a central control module, an electric push rod, and a buzzer. The temperature sensor transmits the real-time temperature to the central control module, which compares the real-time temperature with a preset temperature. If the temperature is higher or lower than the preset temperature, the electric push rod is activated to compress the guide plate, energizing the buzzer and emitting a buzzing sound. This allows relevant personnel to be immediately aware of abnormal temperature inside the culture tank and take timely measures to ensure the cultivation quality and efficiency of the equipment.