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Triad inactivated vaccine for newcastle disease and bird flu (H9 subtype) and escherichia coli disease and preparation method thereof

A technology for Escherichia coli and chicken Newcastle disease virus, applied in the direction of antibacterial drugs, antiviral agents, pharmaceutical formulations, etc., can solve the problems of economic losses in the breeding industry, mixed infection of chicken flocks, high morbidity and mortality

Active Publication Date: 2013-06-12
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mixed infection of chicken flocks is serious, and the morbidity and mortality are high, which has caused huge economic losses to the breeding industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] ——Preparation of bacteria and viruses for production

[0093] (1) Preparation of virus seeds for production of Newcastle disease virus La Sota strain

[0094] Dilute the virus seeds 10,000 times with sterilized saline, inoculate 10-day-old SPF chicken embryos in the allantoic cavity, 0.1ml per embryo, select chicken embryos that died 72 to 96 hours after inoculation and had obvious disease scars, and harvest chicken embryo liquids respectively (allantoic fluid and amniotic fluid), packed in sterile containers. Mix chicken embryo liquid that has been tested for sterility and has an erythrocyte agglutination value of not less than 1:512 (micro method), quantitatively aliquoted, frozen, and marked with the date of harvest, the generation of the virus, etc.

[0095] (2) Preparation of virus seeds for production of H9 subtype avian influenza virus LG1 strain

[0096] Dilute the virus seed 1000 times with sterilized normal saline, inoculate 10-day-old SPF chicken embryos in...

Embodiment 2

[0101] - vaccine preparation

[0102] (1) Antigen solution preparation

[0103] 1) Preparation of Chicken Newcastle Disease Virus Liquid

[0104] ① Inoculate the seed virus used for production, dilute it 10,000 times with sterilized saline, inoculate 9-11-day-old susceptible chicken embryos in the allantoic cavity, 0.1-0.3ml per embryo, seal the pinhole after inoculation, and place at 36-37 Continue to incubate at ℃ without turning the eggs.

[0105] ②Incubation and observation After inoculation of chicken embryos, the eggs were illuminated once a day, and the chicken embryos that died before 60 hours were discarded. Afterwards, the eggs were illuminated every 4-6 hours, and the dead chicken embryos were taken out at any time until 96 hours, regardless of whether they were dead or not, all of them were taken out, the air chamber was upright, and cooled at 2-8°C for 4-24 hours.

[0106] ③Harvesting, inactivation and concentration Take out the cooled chicken embryo, disinfect...

Embodiment 3

[0122] ——Inspection of finished vaccines

[0123] The vaccines used in this example are 6 batches of Newcastle disease, avian influenza (H9 subtype), and Escherichia coli triple inactivated vaccines prepared by the applicant according to the technical scheme provided by the present invention, and the batch numbers are 301, 302, 303, 304 , 305 and 306 batches.

[0124] (1) traits

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PUM

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Abstract

The invention relates to triad inactivated vaccine for newcastle disease, bird flu (H9 subtype) and escherichia coli disease and a preparation method thereof. The vaccine is an effective coping strategy aiming at a prevalence situation of mixed infection of the newcastle disease, the H9 subtype bird flu and the escherichia coli, and two kinds of disease can be controlled at the same by means of immunization at one time. According to the vaccine, a La Sota strain of a newcastle disease virus, a LG1 strain of an H9 subtype bird flu virus, a QL1 strain (O1) of bird source escherichia coli, QL2 strain (O2) of the bird source escherichia coli, QL78 strain (O78) of the bird source escherichia coli, and QL36 strain (O36) of the bird source escherichia coli serve as vaccine reserve virus strains, and the vaccine is formed through antigen liquid preparation, inactivation, concentration and mixing according to a certain proportion. The vaccine and the preparation method are related with the La Sota strain of the newcastle disease virus, the H9 subtype bird flu virus and the bird source escherichia coli which are all domestic prevalence virus strains, and current prevalence disease can be effectively prevented.

Description

technical field [0001] The invention relates to a triple inactivated vaccine of chicken Newcastle disease, avian influenza (H9 subtype) and Escherichia coli, belonging to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) is an acute and highly contagious disease of poultry caused by Newcastle disease virus (NDV). Clinically, Newcastle disease often presents as a course of sepsis, the main features of which are dyspnea, diarrhea—discharge of green or yellow loose feces, nervous dysfunction—paroxysmal convulsions, head and neck torsion, opisthotonus, ataxia, and numb symptoms, mucous membranes and Serosa hemorrhage and necrosis. The disease has an acute onset and a high mortality rate, posing a serious threat to the development of the poultry industry. It has been designated as a Class A severe infectious disease by the International Veterinary Bureau and has always been one of the most important diseases affecting the poultry i...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/16A61P31/04
Inventor 鲍海忠鞠洪涛宋晓飞王蕾徐龙涛吴昊
Owner QILU ANIMAL HEALTH PROD
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