Plant bacteriostatic composition and application thereof in liquid detergent
A plant antibacterial and composition technology, which is applied in the direction of detergent composition, application, plant growth regulator, etc., can solve the problem of undiscovered laundry detergent antisepsis and antibacterial, achieve good antibacterial effect and reduce the effect of adding amount
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Embodiment 1
[0028] Embodiment 1, the preparation of perilla extract, mountain silver flower extract and Jiulixiang extract
[0029] In this example, the experimental raw materials of perilla, mountain silver flower, and Jiulixiang were purchased from the common people's pharmacy. Prepare perilla extract, mountain silver flower extract and Murata extract respectively according to the following methods:
[0030] The perilla extract is extracted according to the following method: the dried perilla stems and leaves are ground and crushed and then passed through a 40-mesh sieve. According to the ratio of 50 mL of extraction solvent per gram of perilla stems and leaves, 90% concentration of ethanol is used as Extract the solvent, reflux at 65°C for 2-3 hours, filter, collect the filtrate, and record it as the first filtrate; add ethanol with a concentration of 90% that is basically the same volume as the first filtrate to the filter residue, and use the same volume as the first filtrate to extr...
Embodiment 2
[0042] The antibacterial experiment of embodiment 2 plant antibacterial composition of the present invention
[0043] 1 material
[0044] 1.1 Test material
[0045] The perilla extract, the mountain silver flower extract and the Murata extract prepared in Example 1.
[0046] 1.2 Tested strains
[0047] Bacteria: Staphylococcus aureus: (Staphylococcus aureus) ATCC 6538
[0048] Escherichia coli: (Escherichia coli) ATCC 8739
[0049] Pseudomonas aeruginosa: (Pseudomonas.aeruginosa) ATCC 9027
[0050] Mold and yeast: Aspergillus niger ATCC 16404
[0051] Candida albicans ATCC 10231
[0052] Note: The above strains are provided by the Microbial Strain Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.
[0053] 1.3 Test medium
[0054] Mold culture medium: Cha's culture medium, sterilized under high pressure at 115°C for 30 minutes, and made into a slant for later use.
[0055] Bacterial medium: lecithin Tween 80-nutrient agar medium, sterili...
Embodiment 3
[0079] Embodiment 3, the preparation of the laundry liquid containing the plant antibacterial composition of the present invention and its preservative antibacterial challenge experiment
[0080] Take the perilla extract stock solution, the mountain silver flower extract stock solution and the Murata extract stock solution prepared according to the method of Example 1, mix and concentrate to obtain the plant antibacterial composition of the present embodiment, wherein the concentration of Chinese herbal medicine in the raw materials is 0.4g / ml perilla, 0.5g / ml mountain silver flower, 0.15g / ml Gurixiang. After testing, in the concentrated composite extract, the content of rosmarinic acid is about 0.04 mg / ml, the content of flavonoids is about 1.8 mg / ml, and the content of chlorogenic acid is about 1.0 mg / ml.
[0081] The raw material composition of the laundry detergent in this example includes surfactants, auxiliary ingredients and the plant antibacterial composition of this e...
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