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Trichoderma koningiopsis and application thereof in prevention and treatment of litchi anthracnose

A technology of Trichoderma pseudocorningi and microbial strains, applied in the direction of application, fungicides, fungi, etc., can solve the problems of pesticide residue environment, increase of drug resistance of pathogenic bacteria, even drug resistance, pollution, etc., and achieve wide adaptability and good Development and application prospects, the effect of little impact on the ecological environment

Active Publication Date: 2020-12-29
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the prevention and treatment of litchi anthracnose, chemical control is the main method. Although chemical control can achieve a certain effect in a short period of time, the continuous use of chemical agents will easily increase the drug resistance or even drug resistance of pathogenic bacteria. At the same time, it is accompanied by problems such as pesticide residues and environmental pollution.

Method used

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  • Trichoderma koningiopsis and application thereof in prevention and treatment of litchi anthracnose
  • Trichoderma koningiopsis and application thereof in prevention and treatment of litchi anthracnose
  • Trichoderma koningiopsis and application thereof in prevention and treatment of litchi anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation, purification and preservation of Trichoderma pseudokoningeni

[0026] (1) Preparation of PDA medium: first wash and peel the potatoes, then weigh 200g of potatoes, chop them into a pot, add 800mL of water to boil for 0.5h, filter with eight layers of gauze, then add 20g of glucose, add water Dilute to 1000mL, stir and mix with a glass rod, add 15g of agar, fully heat and dissolve, then dispense into 250mL Erlenmeyer flasks, sterilize with damp heat at 121°C for 20min, and store for later use.

[0027] (2) Trichoderma strain samples were isolated from the green mold layer on the litchi litchi leaves in the Litchi Base of the College of Horticulture, South China Agricultural University. The strains were isolated and purified with reference to the method of Fang Zhongda et al., and slightly modified: use inoculation Pick a little green mold layer on the PDA medium plate with a needle, place it in an incubator at 28°C and cultivate it. After the gre...

Embodiment 2

[0032] Embodiment 2: confrontation culture method measures the activity of Trichoderma pseudokoningeni

[0033] (1) The preparation of PDA medium is the same as in Example 1.

[0034] (2) Preparation of single colony and bacterial plate of Trichoderma pseudocornifolia: pick Trichoderma pseudocorniensis hyphae on a PDA medium plate with a sterilized toothpick, and culture at 28°C for 3-4 days to obtain a single colony; The edge of Trichoderma pseudoconingi colony is punched to obtain a round fungus disc.

[0035] (3) Cultivation of litchi anthracnose bacteria and preparation of bacteria plates: inoculate the litchi anthrax strains stored at -20°C on a PDA medium plate for activation, and punch them with a puncher with a diameter of 5 mm after the colonies cover the plate Take the edge of the colony to obtain a round disk.

[0036] (4) Activity measurement: on the PDA plate culture medium of 9cm, insert the Trichoderma pseudocornibacterium dish that step (2) prepares and the l...

Embodiment 3

[0038] Embodiment 3: growth rate method measures the activity of Trichoderma pseudokoningenii biological preparation

[0039] (1) The preparation of PDA medium is the same as Example 1; the preparation of PDB liquid medium is except adding agar, and other is identical with the formula of PDA solid medium, and after the medicament weighed is fully dissolved, be subpackaged in conical flasks (each Fill the bottle with 100mL culture solution), stopper, bandage, sterilize with damp heat at 121°C for 20min, cool and store for later use.

[0040] (2) Preparation of Trichoderma pseudocorningii aseptic fermentation filtrate (biological agent): take the Trichoderma pseudocorningii bacteria plate prepared in step (2) of Example 2 and insert into step (1) to prepare the PDB nutrient solution equipped with 100mL Place in a Erlenmeyer flask at 28°C with a shaker speed of 180rpm and shake in the dark for 5 days, then centrifuge at 5000rpm for 10min, absorb the supernatant, and filter out th...

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Abstract

The invention belongs to the technical field of biological prevention and control of plant diseases, and discloses Trichoderma koningiopsis and an application thereof in prevention and control of litchi anthracnose. The strain has a name of Trichoderma koningiopsis TJ5, is preserved in Guangdong Microbial Culture Collection Center, called GDMCC for short, on August 8th, 2020 at Guangdong instituteof microbiology, floor 5, building No.59, courtyard No.100, Xianlie Middle Road, Guangzhou, and has a preservation number of GDMCC No:61129. The strain is antagonistic bacteria screened from Litchi chinensis Sonn, a series of similar survival mechanisms are formed due to long-term coexistence and reciprocal symbiosis of the strain and the Litchi chinensis Sonn, Litchi chinensis Sonn diseases areprevented and treated by utilizing the strain, and the strain is safer and more reliable for eating of the Litchi chinensis Sonn and products processed from the Litchi chinensis Sonn; and the strain has an excellent inhibition effect on litchi anthracnose bacteria, is strong in antibacterial ability, and has important practical significance for preventing and treating the litchi anthracnose.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Trichoderma pseudocorningum and its application in preventing and treating litchi anthracnose. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a subtropical perennial evergreen plant and an important subtropical economic crop, belonging to Sapindaceae. my country's litchi was originally produced in the southern region, including Guangdong, Guangxi, Fujian, Hainan and other provinces. Litchi, as a good product among fruits, has juicy pulp, strong fragrance, tender and refreshing taste. It has long been known as "China's precious fruit" and has extremely high commercial value. However, since litchi fruit ripens mostly in high temperature and high humidity seasons, the fruit after harvesting is prone to browning and rot and deterioration, and litchi anthracnose is the main cause of litchi fruit rot and deteriorat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38C12R2001/885C12N1/145
Inventor 习平根朱洪辉袁玉花李敏慧孔广辉司徒俊键杨文晟姜子德
Owner SOUTH CHINA AGRI UNIV
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