Culture medium and culture method for promoting fast propagation of microalgae

Abstract

The invention discloses a culture medium and a culture method for promoting fast propagation of microalgae. The culture medium for promoting the fast propagation of microalgae is characterized in that hydroxyl-containing three-carbon organic matters are added into the conventional culture medium, the concentration of the hydroxyl-containing three-carbon organic matters in the culture medium is not greater than 1g / L, preferably, the concentration of the hydroxyl-containing three-carbon organic matters is 0.05 g / L to 0.8 g / L, and the hydroxyl-containing three-carbon organic matters comprise glycerin, 1,3-propylene glycol, 1,2-propylene glycol, propyl alcohol, isopropanol, glyceric acid and glyceraldehydes. The culture method for promoting the fast propagation of the microalgae comprises the steps that: microalgae liquid and culture media of the hydroxyl-containing three-carbon organic matters are mixed in a bioreactor according to the volume proportion of 1:15-1:30, the biomass of initial microalgae in the bioreactor is 40 mg / L to 80mg / L, the culture temperature is 20 DEG C to 37 DEG C, the stirring speed is 100 rpm to 500 rpm, and the illumination intensity is 1000 lux to 5000 lux. The culture medium has the advantages that the preparation method is simple, the cost is low, and the culture medium is suitable for industrial application; and when the culture medium is adopted for microalgae culture, the complicated CO2 supply equipment and the illumination condition are not needed, and the autotrophic metabolic efficiency of the microalgae and the CO2 fixing efficiency can be fast improved, so the problem of slow accumulation of microalgae biomass is solved.

Description

technical field [0001] The invention relates to a culture medium and a culture method for promoting rapid proliferation of microalgae. Background technique [0002] Microalgae are a kind of lower plants that grow in water, have a wide variety of species and are extremely widely distributed, and have an efficient photosynthetic reaction system. Microalgae through CO 2 The fixation of light energy can be converted into chemical energy, and stored in the body cells in the form of organic matter such as oil or starch. With the pressure of resource shortage and increasingly severe environmental problems, the use of microalgae to develop biodiesel and some fossil energy substitutes has become a research hotspot. Compared with general autotrophic organisms, microalgae proliferate faster and require less nutrients—mainly sunlight, water and carbon dioxide, which will not cause conflicts with competition with agricultural land and pastoral land. In short, the application prospect ...

AI Technical Summary

Problems solved by technology

The preparation method of the medium is simple, low in cost, and suitable for industrial applications; the culture of microalgae using this medium does not require complicated CO 2 Under the supply of equipment and light conditions, the efficiency of microalgae autotrophic metabolism and CO 2 The immobilization efficiency, thus solving the problem of slow accumulation of microalgae biomass

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