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Marine microbial flocculant and preparation method thereof

A technology for microbial flocculants and marine microorganisms, which is applied in the field of preparation of microbial flocculants, can solve the problems of insufficient scope of use, difficult preparation, and complex microbial culture medium components, and achieves wide application scope, fewer production process steps, and culture conditions. less demanding effects

Active Publication Date: 2019-05-07
山东大湖新材料有限公司
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Problems solved by technology

In recent years, the research on microbial flocculants has been paid more and more attention, but the microbial flocculants currently used have various deficiencies, such as the flocculant disclosed by the patent number CN101121920A, which can only handle wastewater with a pH of 6-7, and the scope of use is not wide enough; and The composition of microbial culture medium is complex and difficult to prepare

Method used

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  • Marine microbial flocculant and preparation method thereof
  • Marine microbial flocculant and preparation method thereof

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preparation example Construction

[0024] A preparation method of marine microbial flocculant, the preparation steps are as follows:

[0025] Step 1. Weigh 45g of starch, 2.5g of ammonium sulfate, 20g of sodium chloride, 2.9g of magnesium sulfate, 4.5g of magnesium chloride, 0.64g of potassium chloride, and 1.75g ​​of calcium chloride, and add them to 1000mL of water, adjust the pH to 6.5, at 112°C Moist heat sterilization for 30 minutes;

[0026] Step 2. Inoculate Amylobacter amylobacterium into a conical flask containing 1000mL liquid medium at a volume ratio of 1:100, and cultivate for 24 hours at a temperature of 25°C and a shaker speed of 150r / min;

[0027] Step 3, centrifuging the culture solution obtained in step 2 at 5000r / min for 30min, removing the precipitate, and the supernatant is the marine microbial flocculant;

[0028] Step 4. Add twice the volume of absolute ethanol to the supernatant obtained in step 3, let it stand at 2-6°C for 12 hours, centrifuge at 9000r / min for 20min, collect the precipi...

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Abstract

The invention provides a marine microbial flocculant. The marine microbial flocculant is produced through fermentation of Amylibacter lutimaris, has a flocculation effect in a wide pH and high salt concentration range, and has a wide application range. The invention provides a preparation method of the marine microbial flocculant. The marine microbial flocculant has a simple liquid medium preparation process, components have wide sources, and are easily available, and the microorganisms have low requirements for culture conditions and a short culture time; the microbial flocculant can be obtained only through fermentation after inoculation is conducted, the production process has few steps, and the dry flocculant product can be obtained through sedimentation, centrifugal separation and drying; and the purification process has simple operation, and the obtained dry flocculant product has the advantages of stable storage, convenient use and the like.

Description

technical field [0001] The invention relates to a method for preparing a microbial flocculant, in particular to a method for preparing a biological flocculant by using amylobacter. Background technique [0002] Flocculation and sedimentation method plays an important role in water supply and sewage treatment. At present, the flocculants widely used in water treatment mainly include inorganic polymer flocculants represented by aluminum series and organic polymer flocculants represented by polyacrylamide. Inorganic flocculants are generally used in large amounts, and non-degradable inorganic ions have certain toxicity, which will seriously pollute the ecological environment and endanger human health; while organic polymer flocculants (such as: polyacrylamide) polymers are not toxic, but Its residues are likely to cause secondary pollution to the ecological environment, and its monomer acrylamide has strong neurotoxicity and is a strong carcinogen. The United States, Britain a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/52C02F1/54C12P1/04C12R1/01
Inventor 贾保磊邵明伟田差沃
Owner 山东大湖新材料有限公司
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