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Bacillus bethecae and application of bacillus bethecae in prevention and treatment of Chinese wolfberry anthracnose

A technology of Bacillus velei and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of preventing and controlling G. pears by the methylotrophic Bacillus strain RT-30 screened by Qian Xinlei et al. (2017) Anthracnose bacteria, Bacillus aracis strain yb33 screened by Han Changzhi and Huo Chao (2015) to control walnut anthracnose bacteria, Bacillus subtilis strain MT332 screened by Cheng Huanhuan et al. Low requirements for culture conditions, excellent biocontrol effect, and strong inhibitory effect

Active Publication Date: 2021-11-02
LANZHOU JIAOTONG UNIV
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Problems solved by technology

At present, there are many reports on the screening of Bacillus as an anthracnose biocontrol bacterium for plants such as pear trees and peppers, such as the methylotrophic Bacillus strain RT-30 screened by Qian Xinlei et al. Han Changzhi and Huo Chao (2015) screened Bacillus aracis strain yb33 to control walnut anthracnose, and Cheng Huanhuan et al. (2019) screened Bacillus subtilis strain MT332 to control pepper anthracnose. Report on Bacteria for the Control of Anthrax

Method used

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  • Bacillus bethecae and application of bacillus bethecae in prevention and treatment of Chinese wolfberry anthracnose
  • Bacillus bethecae and application of bacillus bethecae in prevention and treatment of Chinese wolfberry anthracnose
  • Bacillus bethecae and application of bacillus bethecae in prevention and treatment of Chinese wolfberry anthracnose

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: Screening of biocontrol strains

[0020] The strains F3A, YG, 421, 921, Z9A, and XF isolated from virgin forest soil were activated and cultivated respectively. The plate confrontation method was used to screen the biocontrol strains. The screened out strain F3A had the best bacteriostatic rate to Lycium barbarum anthracnose bacteria, reaching 62.13% (Table 1, figure 2 ).

[0021] Antagonistic effect of table 1F3A on Lycium barbarum anthracnose bacteria

[0022]

[0023]

Embodiment 2

[0024] Embodiment 2: Morphological and molecular biological identification of biocontrol strain F3A

[0025] Morphological identification: observe the morphological characteristics of the biocontrol strains and carry out Gram staining and microscopic examination of spore staining. After being cultured on LB medium at 28°C for 3 days, the diameter of a single colony of the biocontrol strain F3A is about 3 mm, milky white, with irregular edges, and the surface is wrinkled and protruding upwards; the bacteria are rod-shaped, with spores, and Gram staining is positive ( See figure 1 ).

[0026] Molecular biological identification: After the biocontrol strains were activated and cultured on LB medium for 24 hours, single colonies were directly picked and identified by PCR using 16S rDNA and gyrA gene sequences respectively (see Figure 4 ).

[0027] Based on the results of morphological characteristics, 16S rDNA gene sequence analysis and gyrA gene sequence analysis, the biocont...

Embodiment 3

[0028] Embodiment 3: the influence of biocontrol bacterial strain F3A on spore germination

[0029] Lycium barbarum anthracnose bacteria were placed on a PDA plate, cultured in a 25°C incubator for 10 days, and the plate was washed with 10 mL of sterile water to prepare a spore suspension. The screened biocontrol strains were inoculated into 100 mL of LB liquid medium, cultured at 28°C and 180 r / min for 60 hours to obtain a fermentation broth, and the fermentation broth was diluted 1, 10, and 100 times, respectively. Take equal volumes of fermented liquid of biocontrol strains with different dilution factors and mix them with equal volumes of Lycium barbarum anthracnose spore suspension, respectively absorb 100 μL of the mixed solution and place them on concave glass slides. After moisturizing and culturing at 25°C for 24 hours, check the spore germination under a microscope Among them, the inhibition rate of 1-fold dilution was up to 100%, and the inhibition rates of 10-fold ...

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Abstract

The invention discloses bacillus velezensis F3A and application of the bacillus velezensis F3A in biological prevention and control of Chinese wolfberry anthracnose, the preservation number of the bacillus velezensis F3A is CGMCC21518, and the bacillus velezensis F3A is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 23, 2020. The strain has a relatively good prevention and treatment effect on Colletotrichum acutatum, shows broad-spectrum antibacterial activity on pathogenic bacteria such as radix astragali root rot, eggplant sclerotinia rot, tomato leaf mold, tomato early blight and tomato gray mold, has relatively high activity of producing IAA (Indoleacetic Acid), protease and glucanase, and has the capacity of dissolving organic phosphorus and inorganic phosphorus. The new biopesticide and microbial agent developed by using the strain have good application prospects.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a bacillus berysi and its application in the control of wolfberry anthracnose. Background technique [0002] Wolfberry (Lycium chinense) is an important economic crop with the same source of medicine and food, and the planting area in the country has exceeded 3 million mu. Lycium barbarum anthracnose, also known as black fruit disease, is a common disease in the production of wolfberry. It mainly harms the green fruit of wolfberry. Once it becomes popular, it will cause serious economic losses to the growers. The annual output loss is more than 30%, and the serious one can reach 70%. %above. The extensive use of chemical pesticides during the fruit picking period seriously restricts the safe production of wolfberry. Therefore, finding safe and effective biological control measures for wolfberry anthracnose is an urgent problem to be solved in wolfber...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01G7/06A01G13/00A01G17/00C12R1/07
CPCA01N63/22A01G7/06A01G13/00A01G17/005
Inventor 李昭煜李佳佳田永强沈彤田天李国利
Owner LANZHOU JIAOTONG UNIV
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