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A sugarcane endophytic bacillus and its application

A technology for bacilli and amylolytic spores, applied in the field of biological control, can solve problems such as unsatisfactory effect and strain degradation, and achieve the effects of good development and application value, strong inhibitory effect, and low requirements for culture conditions

Active Publication Date: 2021-03-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biocontrol bacteria also have the problem of strain degeneration and unsatisfactory effect.

Method used

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  • A sugarcane endophytic bacillus and its application
  • A sugarcane endophytic bacillus and its application
  • A sugarcane endophytic bacillus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Isolation, purification and preservation of Bacillus amyloliquefaciens CGB15 strain in sugarcane leaves

[0043] 1. Preparation of LB medium

[0044]Weigh 10 g of tryptone (Tryptone, Oxoid LTD LP0042, England), 5 g of yeast extract (Yeasextract, Oxoid LTD LP0021, England), 10 g of sodium chloride (NaCl, Sinopharm Chemical Reagent Co., Ltd., 10019318), add 1000 mL of water and stir well , plus 15g of agar, fully heated to dissolve, subpackaged, sterilized at 121°C for 20min, and stored for later use.

[0045] 2. Isolation and purification of Bacillus amyloliquefaciens in sugarcane leaves

[0046] (1) Leaf collection: The sugarcane leaves used for separation were collected from the sugarcane field of researcher Shen Wankuan of the College of Agriculture, South China Agricultural University, and were roughly divided into new leaves, mature leaves, and old leaves according to the spatial position of the leaves (upper, middle, and lower) Three groups.

[0047] (...

Embodiment 2

[0057] Embodiment 2 confrontation culture method measures the antifungal activity of Bacillus amyloliquefaciens CGB15 bacterial strain

[0058] 1. Preparation of culture medium

[0059] (1) Preparation of LB liquid medium: except that agar is not added, other formulas are the same as those of LB solid medium in Example 1, and the weighed formula medicament is fully dissolved and subpackaged in conical flasks (100mL culture fluid in each bottle) ), stoppered, bandaged, sterilized at 121°C for 20 minutes, cooled and stored for later use.

[0060] (2) Preparation of carrot culture medium: take 200g carrots, filter with 16 layers of gauze after fully squeezing the juice with a juice extractor, add water to 1000mL with the filtrate, add 15g agar, heat and dissolve it, and then pack it into conical flasks ( 100mL culture medium per bottle), sterilized at 121°C for 20min, cooled and stored for later use.

[0061] (3) Preparation of PDA medium: PDA broth (purchased from Dingguo Chan...

Embodiment 3

[0074] Example 3 Control Effect of Bacillus amyloliquefaciens CGB15 on Rice Blast

[0075] 1. The preparation of LB medium is the same as in Example 1; the preparation of CM and PDA medium is the same as in Example 2.

[0076] 2. The preparation of the bacillus plate bacteria suspension and the crude extract is the same as in Example 2.

[0077] 3. Select barley leaves and rice leaves with uniform growth, take the rice blast bacteria cake and cover them on the leaves, set up the clear water group and the fermentation broth group (the extraction process is the same as step 3 in Example 2), and add 5 μL to the bacteria in each group. Between the cake and the leaves. After cultivating in the dark at 28°C for one day, transfer to a 28°C light (12h dark: 12h light) incubator for 3 days, observe the disease and record it, the results are as follows: Figure 6 shown.

[0078] 4. Result analysis

[0079] Depend on Figure 6 It can be seen that the fermentation liquid has an inhib...

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Abstract

The invention belongs to the field of biological pesticides, and discloses a sugarcane endogenous Bacillus amyloliquefaciens CGB15 strain and application thereof. The Bacillus amyloliquefaciens CGB15 strain was deposited in the Guangdong Microbial Culture Collection Center on October 17, 2019, with the preservation number GDMCC No: 60742. The preservation address is: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City. The bacillus amyloliquefaciens CGB15 strain has a strong inhibitory effect on sugarcane whip smut, rice blast fungus, lychee perinospora, lychee anthracnose and banana wilt, and has excellent biological control effect. The preparation and use method of the microbial preparation obtained by the bacterial strain is simple, has broad-spectrum disease resistance, has little impact on the ecological environment, does not affect the quality of crops, and has excellent application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological control, and more specifically relates to a sugarcane endophytic bacillus and application thereof. Background technique [0002] The occurrence of plant diseases refers to the destruction of normal physiological functions of plants due to the influence of other organisms or adverse environmental conditions during the growth and development of plants, thus causing economic losses to agricultural production. At present, several common diseases in the planting process of crops, such as banana fusarium wilt, rice blast, sugarcane smut, etc., have brought serious economic losses to the planting of bananas, rice, sugarcane and other crops. Among the crop diseases, fungal diseases Occupy about 80%, such as Fusarium oxysporum can cause banana wilt, rice blast fungus can cause rice blast, litchi anthracnose can cause litchi anthracnose, litchi downy mildew can cause litchi downy mildew, sugarcane whip b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A61P3/00C12R1/07
CPCC12N1/205C12R2001/07
Inventor 邓懿祯崔国兵尹凯孙文达
Owner SOUTH CHINA AGRI UNIV
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