The invention discloses an opto-acoustic microimaging system. The opto-acoustic microimaging system comprises an exciting light generator, an exciting light path, a detection light generator, a detection light path, a coupling sensor, a light beam decomposition light path and a signal acquisition module. The coupling sensor comprises a metal film, a prism with a convex structure, and a liquid medium. Exciting light acts on a to-be-detected object to generate and return opto-acoustic waves, and accordingly, the refractive index of the liquid medium changes along with time. Detection light comprises components S and P. The prism acts on the metal film to trigger surface plasma resonance, absorption of the component P is influenced by modulation of refractive index changes of the liquid medium, and the detection light is refracted by the prism after the strength of the component P is changed. The detection light is decomposed into the component S and the component P, and the time-dependent opto-acoustic signals are generated according to light intensity difference signals to achieve imaging. Compared with the prior art, the opto-acoustic microimaging system has the advantages that thevisual angle for signal detection is widened by the prism with the concave structure, the opto-acoustic signals away from the optical axis can be received, the field of view for imaging is improved,and acquiring and imaging of the opto-acoustic signals in a large field of view are realized.