Lensless fluorescence microscopic imaging device and image reconstruction method thereof

Abstract

The invention belongs to the technical field of fluorescence microscopic imaging, and relates to a lensless fluorescence microscopic imaging device and an image reconstruction method thereof. The device comprises a monochromatic excitation light source, a movable scattering sheet, a fluorescent sample layer, a high-performance optical filter and an image sensor, wherein the movable scattering sheet is arranged below the monochromatic excitation light source, the fluorescence sample layer is arranged on the lower side of the movable scattering sheet, the movable scattering sheet is parallel tothe fluorescence sample layer, the fluorescence sample layer is used for placing a fluorescence sample to be detected, and the high-performance optical filter is arranged on the lower side of the fluorescence sample layer. The high-performance optical filter is matched with the monochromatic excitation light source and a fluorescent sample of the fluorescent sample layer, and the image sensor is arranged on the lower side of the high-performance optical filter; the lensless fluorescence microscopic imaging device is simple in light path, short in imaging light path, simple and convenient in system operation, large in imaging view field and low in equipment cost; the reconstruction algorithm of the fluorescence sample image is used for processing the fluorescence sample image, and the processed image is high in resolution and good in image quality.

Description

Technical field: [0001] The invention belongs to the technical field of fluorescence microscopy imaging, and relates to a lens-free fluorescence microscopy imaging device based on moving speckles and a reconstruction method for collected images, in particular to a lens-free fluorescence microscopy imaging device and an image reconstruction method thereof. Background technique: [0002] Lensless microscopic imaging technology abandons bulky and expensive optical lenses, and has the advantages of simple structure, small size, easy operation, and low price compared with traditional microscopes. application prospects. [0003] In the prior art, the Chinese Patent Publication No. CN210690407U discloses a ruby ​​fluorescence detection system, including an excitation light source, a first filter, a sample stage, a three-dimensional motorized operating system, a second filter, and a filter A conversion device, a lens, an image sensor and a display screen, the three-dimensional moto...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of large defocus, low resolution and poor imaging quality in the existing lensless microscopic imaging technology, and seek to design a lensless fluorescent microscopic imaging device and its image reconstruction method. Simultaneously achieve large field of view and high resolution fluorescence imaging

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