The invention discloses a multispectral
holographic imaging system and an imaging method. The
system comprises a multi-
wavelength light source module which is composed of a plurality of
light source assemblies which are independently driven and have different wavelengths; the
optical fiber coupling module is composed of a plurality of
optical fiber bundles which are connected with the emergent end face of the
light source assembly and used for restraining optical signals of the light source
assembly as
point light sources. Light signals of the light source
assembly are constrained to the light emitting area to form an equivalent
point light source; the
image acquisition module comprises a sample jig and an
image sensor which are coaxially arranged with the emergent end face of the
optical fiber bundle, and the
image sensor is used for acquiring spectral interferograms of the object light and the reference light; and the control
processing module is used for sequentially controlling the switching of each
wavelength light source assembly, analyzing and
processing a plurality of spectral interferograms, and reconstructing the three-dimensional morphology of the sample through an angular spectrum
diffraction algorithm. The problems of aberration, field depth limitation, view limitation, complex structure, high cost, resolution limitation and the like in the traditional
microscopic imaging technology can be solved.