60 results about "Phase imaging" patented technology

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

To provide an X-ray phase imaging apparatus and an image processing method for improving contrast and visibility of an image photographed by a phase contrast method.SOLUTION: According to an aspect of the present invention, there is provided an X-ray phase imaging apparatus comprising an X-ray source, a detector configured to detect X-rays emitted from the X-ray source to a subject, and an image processing unit configured to generate an image of the subject, the image processing unit includes a storage unit in which a plurality of noise correction images for correcting a luminance variation between pixels are stored separately for brightness and darkness, and an image generation unit that generates a luminance image of the subject by irradiating the subject with X-rays, generates a plurality of corrected images of the subject by correcting the luminance image according to the noise correction image, and generates one subject image by selecting the plurality of corrected images according to a threshold value.SELECTED DRAWING: Figure 5

The present application relates to the field of optical interferometry, and aims at the phase distortion problem in off-axis interferometric quantitative phase imaging, and provides an interferometric microscopic phase distortion elimination method based on PACUNeXt3+ network. The method comprises the following steps: 1. using Zernike polynomials and test target pictures to simulate and generate a data set; 2. establishing and training a PACUNeXt3+ neural network model; 3. inputting the interference graph I or of the sample to be measured into the trained neural network, and outputting the background interference graph I' r corresponding to the sample without sample information; 4. using the two interference graphs I or and I' r to reconstruct the sample phase distribution φ o (x, y) without phase distortion. The present application has high precision and speed, can eliminate the secondary or high-order phase distortion in interferometric quantitative phase imaging, and has great application prospect in the field of phase imaging.

The application relates to a kind of quantitative imaging methods of living cells based on digital holographic microscopic sequential displacement aberration correction, and belongs to the field of optical measurement, comprising: collecting three holograms by sequentially displacing living cells; obtaining three original phase maps by phase demodulation; obtaining total mask map; sequentially displacing phase map in reverse direction; performing difference calculation to obtain global phase system aberration difference data; obtaining local system aberration difference data by using total mask map; performing difference processing on Chebyshev polynomial to obtain local polynomial difference data by using total mask map; fitting global system aberration by calculating corresponding coefficients from difference data; subtracting global system aberration from three original phase maps to obtain phase map of living cells after aberration correction. The application realizes high-precision quantitative phase imaging of living cells while retaining high-frequency information, has excellent displacement distance robustness, does not need to be equipped with high-precision displacement mechanism, and greatly reduces the hardware cost of measurement system.

The present disclosure provides a pathology sample virtual staining apparatus and method that can easily acquire virtual staining sample images for unlabeled samples as well as decolorized samples, by including a sample image acquisition module that irradiates near-infrared light toward a placed sample to acquire a near-infrared-based phase image of the sample, and a virtual staining module that receives the near-infrared-based phase image and performs neural network computation to acquire a virtual staining image similar to an image of a stained sample in which the sample is stained.

A quantitative phase contrast imaging system comprises a light source configured to generate light and to direct the light to a sample, wherein the light comprises nonlinearly polarized light; a first lens configured to receive light from the sample; a phase filter, wherein the first lens is configured to focus the light from the sample onto the phase filter, and the phase filter is configured to modulate multiple components of the nonlinearly polarized light simultaneously (e.g., geometric phase interferometry); a second lens configured to receive light from the phase filter; and a polarized camera, wherein the second lens is configured to collimate the light beam from the phase filter to the polarized camera and the polarized camera is configured to image the sample in response to the light from the phase filter. In absence of the second lens, the camera can be placed at the image plane.

The invention discloses an SPR phase imaging system based on a four-polarization filtering array, and the system comprises an incident light path which is used for generating area array laser; the SPR module is used for receiving the area array laser and carrying out SPR sensing to obtain an area array laser modulation result related to a to-be-detected substance; and the imaging light path is used for carrying out amplification and filtering modulation on the area array laser modulation result so as to carry out imaging on a four-polarization array, and carrying out matched filtering and double-differential phase resolving on a four-polarization image so as to obtain an imaging result. According to the invention, through polarization virtual image generation and cross-channel block matching, and in combination with four-dimensional collaborative filtering, the noise suppression efficiency is improved; and through double differential phase resolving, the dynamic range is expanded to 0.06 RIU, and the resolution ratio is superior to that of the RIU. The invention solves the problem of mutual restriction of dynamic range and resolution in the traditional SPR technology, and is suitable for high-sensitivity biomolecule interaction detection.

Mesoscopic imaging systems and methods comprise or utilize a light source configured to generate an illumination light; an optical system configured to direct the illumination light toward a sample; an objective lens having a numerical aperture (NA) of 0.2 to 0.6, the objective lens being configured to receive a response light emitted by the sample in response to the illumination light; a filter wheel comprising a plurality of emission filters configured to filter the response light to generate a filtered light; a tube lens configured to provide an optical magnification to the filtered light; and an image sensor having 40 megapixels or more and a pixel size of less than or equal to 4 μm, the image sensor being configured to receive the filtered light and generate image data based on the received filtered light.

The application relates to the technical field of mine evaluation, in particular to a mine available resource evaluation method and system.The method comprises the following steps: obtaining mine resource geological exploration data; performing data preprocessing on the mine resource geological exploration data to generate standard mine resource geological exploration data; performing three-dimensional geological modeling based on the standard mine resource geological exploration data to generate mine resource reserve distribution data; obtaining a surface deformation monitoring image through a synthetic aperture radar; performing time sequence slicing on the surface deformation monitoring image to generate surface deformation slice data; and performing stress change and microseismic phase imaging on the surface deformation slice data, so that time sequence stress data and microseismic phase data are obtained.The application improves the accuracy, dynamic nature and geological risk early warning capability of mine resource evaluation through multi-source dynamic data fusion and three-dimensional modeling, and improves the precision of mine available resource evaluation.

The application discloses an imaging sensor based on a full-Stokes polarization superlens, and relates to the field of optical sensing.The imaging sensor comprises a sensing excitation light source, an adjustable attenuator, a polarizer, a coupling prism, a sensing chip, a first objective lens, a full-Stokes polarization superlens, a second objective lens, an imaging lens and a CCD imaging camera; the sensing excitation light source emits monochromatic light as system incident light, which sequentially passes through the adjustable attenuator and the polarizer, and is incident onto the surface of the sensing chip to generate surface plasmon resonance after passing through the coupling prism; reflected light is converged onto the polarization superlens by the first objective lens, and then converged onto the second objective lens; subsequently, the imaging lens is used for imaging to the CCD camera; the property change of a to-be-measured sample leads to surface plasmon resonance of the sensing chip, which causes the analysis information of the full-Stokes polarization superlens to change, and further causes the image obtained by the CCD imaging camera to change.The application can perform single-shot phase imaging on a refractive index sample.

This invention discloses a lensless fiber bundle phase imaging method. The method includes: acquiring a single-exposure image of a sample through an optical fiber under multi-wavelength illumination; obtaining light intensity data for reconstruction by multi-channel separation and correction of the image; reconstructing the diffraction image of the sample based on the obtained light intensity data by removing the fiber bundle structure; establishing a lensless fiber bundle imaging model and correlating the diffraction image with the complex amplitude distribution of the sample; constructing an objective function for optimization and solving to obtain the amplitude image and phase image of the sample. This invention eliminates the need for lenses in the fiber bundle, offering advantages such as simple structure and suitability for miniaturized systems, and enables stable quantitative phase imaging under single exposure.

The invention discloses a use method of a scanning splicing large-view-field fundus phase imaging device. The scanning splicing large-view-field fundus phase imaging device comprises an illumination light path and a zoom light path. The illumination light path comprises a scanning mirror, a spectroscope and a fundus objective lens, the front side of the scanning mirror is a collimation light source, the rear side of the scanning mirror is the spectroscope, the left side of the spectroscope is the fundus objective lens and a patient fundus, and the right side of the spectroscope is a zoom lens group of a zoom light path and a fundus camera. The scanning mirror is rotated and the focal length of the zoom lens group is adjusted in sequence according to the plurality of scanning sequence groups, so that the fundus camera acquires a plurality of groups of positive defocus state, focusing state and negative defocus state images, then a plurality of local fundus phase diagrams are obtained according to the images, and a complete fundus phase distribution diagram is obtained, namely, the fundus phase distribution diagram has the phase information acquisition capability; and observing microscopic important pathological features according to the fundus phase distribution diagram. Therefore, the design has the capability of acquiring phase information, and microscopic important pathological features can be observed.

An apparatus, system, and related methods for the conversion of a regular bright-field microscope into a PS-QPI (Polarization-Sensitivity and Quantitative Phase Imaging) microscope system. A regular bright-field microscope is converted by insertion of a polarizing element in the illumination path and a Fresnel biprism in the image space of the system. The converted system is suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes, as well as identification of cell / tissue screening and diagnosis.

This invention discloses a quantitative phase imaging system and method for embryos based on dual liquid zoom lenses, which is installed in a time-difference incubator. It includes an illumination module, an imaging module, and a quantitative phase imaging module arranged sequentially along the optical path. The illumination module includes an LED light source, an illumination slit modulation structure, and a liquid zoom focusing system. The liquid zoom focusing system dynamically adjusts the numerical aperture of the illumination by changing the focal length. The imaging module includes an objective lens, a polarizer, a Nomaski prism, an analyzer, and a liquid imaging lens. The polarizer is positioned in the output optical path of the objective lens, the Nomaski prism is positioned between the polarizer and the analyzer, and the liquid imaging lens is positioned in the output optical path of the analyzer. The quantitative phase imaging module includes a spatial light modulator and an image acquisition unit. The spatial light modulator is used to perform phase-shift modulation on the optical path.

The invention provides a lensless amplitude phase imaging method and system suitable for axial scanning of polychromatic light. The method comprises the following steps: respectively acquiring diffraction data of an optical diffraction element and sample diffraction data at different positions through a lensless imaging device; randomly selecting a sample plane, transmitting a probe function of the optical diffraction element plane to the sample plane, transmitting a transmission function of the sample plane to a detector plane, and updating the transmission function through sample diffraction data; reversely transmitting the updated transmission function back to the sample plane, respectively updating the probe function and the object function of the sample, and transmitting the probe function back to the optical diffraction element plane; transmitting the probe function to a detector plane, performing amplitude constraint on the probe function by using diffraction data of the optical diffraction element, and then transmitting the probe function back to the plane of the optical diffraction element; and repeating the above steps to complete iteration, and finally obtaining a structure diagram through reconstruction. According to the invention, data volume is reduced and imaging quality is improved.

A synthetic aperture quantitative phase imaging method based on a hybrid of digital holography and Fourier ptychography. A programmable LED array which is annularly arranged is mounted on an off-axis digital holographic system for providing oblique illumination at varying angles; accurate noise-containing low-frequency information of an object is obtained by means of digital holography and is used as initial estimation; and then, high-frequency information of the object is reconstructed by means of a Fourier ptychographic algorithm based on intensity measurement, so as to realize synthetic aperture quantitative phase imaging. Using the accurate low frequency provided by holography as initial estimation ensures the accuracy of phase retrieval and wavefront aberration reconstruction, without strictly meeting matching illumination conditions, and reduces the number of intensity graphs required by Fourier ptychography; and a non-interferometric phase retrieval method based on Fourier ptychography improves the imaging resolution to an incoherent diffraction limit, and also effectively solves the problem of inherent speckle noise in digital holography.

The invention discloses a microfluidic quantitative phase imaging system and method based on an event camera, and the method employs the event camera to replace a frame camera in a conventional imaging system, and employs a working mechanism that the event camera is only sensitive to light intensity changes and asynchronously outputs event streams. Foreground extraction of a moving sample in the micro-fluidic chip and inhibition of a static background are realized, and background redundancy is eradicated from the source of data acquisition. According to the method, the inherent contradiction between high throughput and data explosion in the traditional technology is solved, and a brand new implementation way with zero data redundancy and high time resolution can be provided for high-precision, high-throughput and long-time dynamic quantitative phase measurement and analysis.

The invention relates to the technical field of chronic disease information intelligent management, and discloses an AI-based multi-disease chronic disease information intelligent management system, and the system comprises a video collection module which collects original frames, screens out invalid frames according to the definition, and constructs an effective sequence; the space-time registration module is used for constructing a Lagrange view angle and removing noise in combination with spectrum gating; the signal decoupling module is used for separating hemoglobin and constructing an enhanced blood flow signal according to texture weighting; the phase imaging module is used for calculating a lag amount and generating a microcirculation phase thermodynamic diagram; the standardized mapping module is used for identifying the mark points and generating a standardized phase diagram without rigid mapping; and the risk assessment module is used for executing historical difference, analyzing disease course evolution, outputting risks and prompting. According to the method, the problem of detection distortion caused by common limb tremor or inautonomous movement of the chronic disease patient is effectively solved by constructing the Lagrange view angle video stream and combining the space-time spectrum gating technology.

This invention discloses a differential interferometric phase-contrast imaging system, method, and time-difference incubator device, belonging to the fields of optical imaging and medical device technology. The system includes an illumination source module, a microscopic imaging module, a polarization interferometry module, and a quantitative phase image reconstruction module. By placing all polarization interferometry components, such as the polarizer and polarization beam splitter, on the image side of the microscopic imaging module, the imaging beam does not need to pass through the plastic culture dish before passing through the polarization elements, thus completely avoiding interference from plastic birefringence on the imaging and achieving high-quality observation of samples in cell biology or materials science fields within plastic culture dishes. Simultaneously, the system uses a polarization camera to simultaneously acquire multiple phase-shifted interferograms in a single exposure, combined with a quantitative phase reconstruction algorithm, achieving high-speed, high-precision quantitative phase imaging, overcoming the shortcomings of traditional mechanical phase-shifting methods, such as slow speed and susceptibility to motion artifacts. Furthermore, the imaging system of this invention can also be integrated into a time-difference incubator.

The invention relates to a method and a device for detecting a drivable area (2) in the surroundings of a vehicle (1), in which an image of the surroundings of the vehicle (1) is captured by means of a sensor device, and in which the drivable area (2) is detected in the image, in which a spatial phase imaging sensor device is used as the sensor device for capturing the surroundings of the vehicle (1), a travelable area (2) is distinguished from a non-travelable area (3) on the basis of normal information and distance information from the detected area.

The invention provides a high-speed detection imaging system and method for large-aperture optical mirror defects, and belongs to the technical field of high-speed imaging detection. The system mainly comprises a detection light source unit, a phase imaging unit and a signal processing unit, the detection light source unit is used for generating a time domain stretching ultrafast laser pulse, the phase imaging unit is used for scanning and detecting an optical mirror surface, and the signal processing unit is used for recovering an acquired signal, so that the intensity and phase image reconstruction of the optical mirror surface is realized. According to the invention, the imaging speed limitation of traditional camera detection can be broken through, the megahertz imaging detection frame rate of the optical mirror surface is achieved, and meanwhile, the strength and phase images are combined, so that the online continuous high-precision and high-sensitivity detection of the defects of the optical mirror surface is realized.

The invention relates to a lensless coding lamination quantitative phase imaging system and method, in particular to a lensless coding lamination quantitative phase imaging system and method based on multi-angle illumination. The system comprises a laser light source, a barrel lens and a coding sensor, the laser light source is arranged at the focal plane of the barrel lens and is used for scanning the barrel lens to different transverse positions and generating incident plane waves with different angles; the coding sensor is arranged on an emergent light path of the barrel lens and is used for image acquisition; a coding layer is arranged above the photosensitive surface of the coding sensor; and a sample to be imaged is arranged between the barrel lens and the coding sensor during imaging. According to the method, the problems that low-frequency phase information is lost and quantitative reconstruction of a plurality of 2pi wrapped phase samples is difficult to realize in the traditional quantitative phase imaging technology can be solved; and the system has large field of view, high resolution and quantitative phase imaging capability, and is highly suitable for related applications such as biomedicine, pathological analysis and telemedicine.

The invention relates to an automobile power battery shell micropore nondestructive testing method based on synchrotron radiation X-ray phase imaging, and relates to the technical field of photoelectric detection / image recognition / nondestructive testing. The method comprises the following steps: constructing a uniform irradiation field and acquiring data; phase inversion and electron cloud density reconstruction; feature fusion and abnormal region identification; performing defect classification and risk assessment; and performing parameter feedback and system optimization. According to the automobile power battery shell micro-pore nondestructive testing method based on synchrotron radiation X-ray phase imaging, phase change caused by electron cloud density disturbance is imaged by utilizing high coherence of synchrotron radiation X-rays and a phase contrast imaging principle; the sensitivity to the internal microstructure of the low-atomic-number material is several orders of magnitude higher than that of absorption imaging, micropores which cannot be seen by a traditional method can be clearly recognized, and the detection sensitivity is improved.

The invention discloses a lensless optical fiber bundle phase imaging method. The method comprises the following steps: acquiring a single exposure image of a sample through an optical fiber under multi-wavelength illumination; obtaining light intensity data for reconstruction through multi-channel separation and correction processing of the image; based on the obtained light intensity data, removing an optical fiber bundle structure, and reconstructing a diffraction image of the sample; establishing a lensless optical fiber bundle imaging model, and associating the diffraction image with the complex amplitude distribution of the sample; and constructing an objective function for optimization solution to obtain an amplitude image and a phase image of the sample. A lens does not need to be arranged in the optical fiber bundle, the device has the advantages of being simple in structure, suitable for a miniaturized system and the like, and stable quantitative phase imaging can be achieved under single exposure.

Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for identifying the predicted type of one or more microorganisms. In one aspect, a system comprises a phase-contrast microscope and a microorganism classification system. The phase-contrast microscope is configured to generate a three-dimensional quantitative phase image of one or more microorganisms. The microorganism classification system is configured to process the three-dimensional quantitative phase image using a neural network to generate a neural network output characterizing the microorganisms, and thereafter identify the predicted type of the microorganisms using the neural network output.

The invention provides an optical body microscopic imaging device and method based on programmable LED array illumination and a quantitative phase imaging camera, and solves the problem that a traditional optical microscope is difficult to obtain a three-dimensional structure in a transparent sample such as cells; and the technical problems of high imaging noise, poor system stability and high operation and environment requirements of the existing optical diffraction tomography microscope are solved. The device comprises a programmable LED array, a microscopic imaging unit and a quantitative phase imaging camera with a built-in storage unit, the programmable LED array sequentially emits light beams to irradiate a sample on the microscopic imaging unit, the light beams become object light carrying sample information after penetrating through the sample, after amplification and interference, four-wave shear interference images are collected through the quantitative phase imaging camera, and the sample information is obtained. And finally determining the refractive index distribution of the sample according to an image processing program and an optical diffraction tomography reconstruction algorithm. The method has the advantages of low noise and high imaging quality.

The application discloses a SPR phase imaging system based on an inclination angle spectrum, comprising: an incident light path for generating a surface array laser and being incident at a preset inclination angle; an SPR module for receiving the surface array laser and performing SPR sensing to obtain a surface array laser modulation result related to a substance to be measured; an imaging light path for spatially amplifying and light filtering the modulation result to form an image on a four-polarization array; a data processing unit for performing matched filtering and double-differential phase calculation on the four-polarization image to obtain a preliminary imaging result; constructing an SPR imaging process equivalent model based on the measured light field intensity information containing the inclination angle of the four-polarization array, inputting the preliminary imaging result into the model to obtain the light field information of p-polarized light, and performing inclination angle spectrum modeling and reverse propagation on the light field information of the p-polarized light based on an angle spectrum propagation theory to obtain a clear imaging result. The application improves the global spatial resolution under a large field of view to the level of a focusing region.

A 3D surface map of a workpiece is determined using an interferometric quantitative phase imaging technique. The workpiece includes a transparent thin film or layers stack. The 3D surface map is corrected based on a thickness and a refractive index of the transparent thin film or layers stack. This technique can be used with an inspection system configured to perform an interferometric quantitative phase imaging.

The invention relates to a method and a device for detecting a dynamic behavior of a trailer or semitrailer (1) of a commercial vehicle combination (10), in which an image of the trailer or semitrailer (1) is captured by means of a sensor system, at least one side (2) of the trailer or semitrailer (1) being captured by means of a sensor system designed as a spatial phase imaging sensor system, according to the invention, a flat side (2) is identified on the basis of the same normal vector at at least approximately each position of the side (2) or at least very similar normal vectors in a specific range, and uneven or biased sides (2) are identified on the basis of different normal vectors at different positions of the side (2).

The invention discloses a quantitative phase imaging system and method based on Bessel beam illumination, and the method comprises the steps: enabling laser output by a laser device to be coupled through a beam splitting 1 * 2 single-mode optical fiber through a light source and a beam splitting module, filtering a high-order mode, and splitting the laser into object light and reference light; through a Bessel beam generation module, object light is collimated by a short-focus lens to form a plane wave, the plane wave is incident to a spatial light modulator, phase distribution of an axis prism is loaded, the plane wave is modulated into a Bessel beam, the Bessel beam is subjected to beam contraction through an objective lens and a tube lens in sequence, the Bessel beam passes through a scattering medium, an effective area covered by a central main lobe serves as an imaging area, a sample to be detected is irradiated, and the target object is detected. Outputting central main lobe object light; the reference light is collimated into a plane wave through a short-focus lens, the incident angle of the plane wave is adjusted through a reflector, then the intensity of the reference light is adjusted through a linear polarizer, and interference fringes with the optimal contrast are obtained; and the signal acquisition and processing module converts data extracted from the interference fringes into three-dimensional height information of the sample, so that high-precision quantitative phase imaging is realized.