Differential phase contrast quantitative phase microscopic imaging method based on optimal illumination mode design

Abstract

The invention discloses a differential phase contrast quantitative phase microscopic imaging method based on optimal illumination mode design. According to the method, an optimal illumination patterncorresponding to an isotropic phase transfer function in differential phase contrast quantitative phase imaging is deduced, the pattern is determined as a semi-circular illumination pattern whose illumination numerical aperture NAill is equal to a system object lens numerical aperture Naobj, the illumination intensity distribution starts from a direction axis and varies with the cosine of an illumination angle, and the intensity distribution can be expressed as S(theta)=cos(theta) in polar coordinates. According to the method, the frequency loss of the phase transmission is effectively compensated, the transmission performance of a highest frequency is enhanced, at the same time, the transmission property of low-frequency phase information is also significantly improved, the correctness and high resolution of a phase result are ensured, at the same time, the number of illumination axes is reduced to two by an optimal illumination scheme, the number of collected images needed by differential phase contrast quantitative phase imaging is greatly reduced, the imaging speed is improved, and a real-time dynamic phase imaging result with high accuracy and high resolution is obtained.

Description

technical field [0001] The invention belongs to optical microscopic imaging and measurement technology, in particular to a differential phase contrast quantitative phase microscopic imaging method based on optimal illumination mode design. Background technique [0002] Due to the transparent nature of biological cells, label-free research on biological cells has always been a hot spot in microscopy imaging methods. Quantitative phase imaging technology, as the most diverse and widely used label-free imaging method, has gradually become the main means of cell research. In the quantitative phase imaging technology, it can be mainly divided into two categories according to the imaging principle, that is, quantitative phase imaging methods based on coherent imaging and incoherent imaging. Benefiting from different imaging principles, these two methods often have different imaging performances in terms of imaging resolution, imaging throughput, and imaging speed. Among these im...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a real-time dynamic, high accuracy, high-resolution differential phase contrast quantitative phase microscopy imaging method under the optimal illumination mode to solve the lack of partial frequency information of the phase imaging results in differential phase contrast quantitative phase imaging Serious, slow imaging speed, system is sensitive to noise, etc.

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