A fast wide-field volume holographic fluorescence microscopy imaging system

Abstract

The invention relates to a fast wide-field volume holographic fluorescent microscopic imaging system, which is characterized in that it includes a laser light source, a dichroic beam splitter, a microscopic objective lens, a MEMS microreflector array device, a volume holographic grating device, and an imaging lens and the image detector array; the laser light source emits the illumination light wave to the dichroic beam splitter; the dichroic beam splitter irradiates the illumination light wave through the microscopic objective lens to the imaging target, and the fluorescence emitted by the imaging target returns to the dichroic beam splitter through the microscopic objective lens, The microscopic objective lens projects the fluorescence to the MEMS micro-mirror array device; the MEMS micro-mirror array device encodes the angle of the light waves at different positions according to the central wavelength of the imaging spectrum and the Bragg characteristic parameters of the volume holographic grating device, and then controls the angle of the imaging beam. The deflection direction, the deflected imaging beam is incident on the volume holographic grating device at a matching angle; the volume holographic grating device diffracts the incident light encoded by the spatial angle, and the diffracted light is decoded by the MEMS micro-mirror decoding array device and then passes through the imaging lens Imaging onto the image detector array.

Description

technical field [0001] The invention relates to the technical field of spatial spectrum microscopic imaging, in particular to a fast wide-field volume holographic fluorescent microscopic imaging system. Background technique [0002] The spectral microscopic imaging system based on volume holographic grating is a new microscopic imaging technology that has attracted much attention in recent years. It has a very broad application in the fields of shallow tumor detection, disease diagnosis, food and drug supervision, biological inspection and quarantine, and material science Application prospects. The volume holographic microspectral imaging system uses the Bragg selection characteristics of the volume holographic grating device to realize microscopic imaging with high spectral resolution. The volume holographic grating device is made by using a photorefractive material with a certain thickness to record the coherent pattern of two beams of coherent light in the recording mate...

AI Technical Summary

Problems solved by technology

Due to the Bragg selection characteristics of the volume holographic grating, under the narrow-band light illumination condition, the pattern obtained on the image sensor is a narrow band. If you want to obtain a wide-field image of a single color light, you need to use a horizontal scanning method, but this solution increases The complexity of the system reduces the imaging efficiency, and it cannot realize the continuous and fast switching of multiple monochromatic spectra with a large field of view

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