Bright field phase microscopic imaging device and method based on spiral phase plate

Abstract

The invention provides a bright field phase microscopic imaging device and method based on a spiral phase plate. The device comprises: a laser light source; a beam expanding and collimating unit usedfor carrying out beam expanding and collimating on the laser beam and projecting the laser beam onto a sample to generate Gaussian light carrying sample information; a phase modulation unit used for carrying out phase modulation on the Gaussian light according to the imported spiral phase plate hologram and generating vortex light carrying sample information; a detector used for collecting vortexlight and obtaining an imaging intensity graph; and a control terminal used for processing the imaging intensity image to obtain a reconstructed sample phase information image. According to the invention, phase modulation is carried out on the Gaussian beam carrying the sample information, and the collected Gaussian beam is converted into the vortex light, so that the sample phase information canbe obtained by shooting and recording the imaging intensity graph once, the device is simple, the operation is convenient, the imaging speed is high, the imaging field of view is large, the limitationof dark field imaging is eliminated by bright field imaging, the time resolution of phase microscopic imaging is greatly improved, and the imaging steps are simplified.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and in particular relates to a bright-field phase microscopic imaging device and method based on a spiral phase plate. Background technique [0002] Although the traditional optical microscope can achieve label-free observation, it relies on the optical properties of the sample itself and lacks specificity. Although the subsequent development of the fluorescence microscope broke the limitation of the far-field diffraction limit on the resolution, the improvement of the spatial resolution of the fluorescence microscope was often at the expense of the time resolution, and the fluorescence microscopy technique had high phototoxicity and phototoxicity. The bleaching and staining process is complicated and time-consuming, and fluorescent proteins or fluorescent dyes will change the normal physiological state of cells, and the labeled cells can no longer enter the human body. These disadvantage...

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Problems solved by technology

[0005] In view of the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a bright-field phase microscopic imaging device and method based on a spiral phase plate, which overcomes the need to collect multiple imaging intensity maps for existing phase imaging, and the imaging process consumes a lot of time. when the defect

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