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Strain of bacillus altitudinis and application thereof in prevention and treatment of peronophythora litchii

A technology of Bacillus alpinus and litchi, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of drug resistance of pathogenic microorganisms, achieve good development and application prospects, strong inhibitory effect, and low requirements for culture conditions

Active Publication Date: 2019-11-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This not only leaves a potential risk for the edible safety of lychee fruit, but also promotes the development of drug resistance of pathogenic microorganisms

Method used

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  • Strain of bacillus altitudinis and application thereof in prevention and treatment of peronophythora litchii
  • Strain of bacillus altitudinis and application thereof in prevention and treatment of peronophythora litchii
  • Strain of bacillus altitudinis and application thereof in prevention and treatment of peronophythora litchii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation, purification and preservation of Bacillus alpina

[0026] (1) Preparation of LB medium: Weigh 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, add 1000mL of water and stir to mix, then add 15g of agar, fully heat and dissolve, then pack into 250mL Erlenmeyer flask, 121℃ Sterilize with moist heat for 20 minutes and save for later use.

[0027] (2) Isolation and purification of Bacillus alpina: The soil samples used to isolate antagonistic bacteria were collected from the rhizosphere soil of healthy fruit trees in the litchi orchard of South China Agricultural University. Weigh 10 g of soil sample, add the soil sample into a Erlenmeyer flask filled with 90 mL of sterile saline (0.9%) under aseptic conditions, place it on a shaker at 180 rpm at 28 ° C for 30 min, and prepare a dilution factor of 10 -1 Soil dilution solution, let it stand at room temperature for 10 minutes to separate solid impurities such as sand, stone, and soil. Ta...

Embodiment 2

[0032] Embodiment 2: confrontation culture method measures the activity of Bacillus alpinus

[0033] (1) The preparation of LB medium is the same as in Example 1.

[0034] (2) Preparation of carrot culture medium: take 200g carrots, fully extract the juice with a juicer, filter with 16 layers of gauze, make up the water to 1000mL with the filtrate, add 15g agar, heat and dissolve it, and then pack it into conical flasks. After sealing, sterilize with damp heat at 121°C for 20 minutes, and store for future use.

[0035] (3) Preparation of a single colony of Bacillus alpina: Streak inoculation of Bacillus on an LB medium plate for activation and culture for 24 hours, pick a single colony for streak preservation.

[0036] (4) Cultivation of Pythophthora lychee: inoculate Pythophthora lychee on a carrot plate, and after culturing for 7 days, use a puncher with a diameter of 7 mm to punch out the fungus cake at the edge of the colony.

[0037] (5) Activity measurement: the bacill...

Embodiment 3

[0038] Embodiment 3: Oxford cup method assays the activity of Bacillus alpina biological preparation

[0039] (1) The preparation of the LB medium is the same as in Example 1; the preparation of the LB liquid medium is except that agar is not added, and the other is the same as the formula of the LB solid medium, and the weighed medicament is fully dissolved and packed in conical flasks (per bottle (100mL culture solution), stoppered, bandaged, sterilized at 121°C for 20min, cooled and stored for later use.

[0040] (2) The preparation of the carrot culture medium is the same as in Example 2.

[0041] (3) Preparation of Bacillus alpinus biological preparation: take the single colony of Bacillus alpinus prepared in step (3) of Example 2 and insert it into the LB culture medium Erlenmeyer flask prepared in step (1), place it at 30°C on a shaker The rate is 200 rpm and cultured for 24 hours to obtain the biocontrol agent of Bacillus alpina.

[0042] (4) The cultivation of Lyche...

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Abstract

The invention belongs to the technical field of plant disease biocontrol, and discloses a strain of bacillus altitudinis XJGS-4 and an application thereof in prevention and treatment of peronophythoralitchii. The bacillus altitudinis is named as bacillus altitudinis XJGS-4, is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) at Guangdong Institute of Microbiology, 5 floor, No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou City, has the preservation number of GDMCC No:60698, and is preserved on June 17, 2019. The obtained bacillus altitudinis comes from rhizosphere soil of healthy litchi chinensis sonn fruit trees, is mutually symbiotic with litchi chinensis sonn for a long time, and avoids the possibility of damaging of the stability of a micro ecological environment on a plant surface; the bacillus altitudinis and a biological preparation based on the bacillus altitudinis have a strong inhibitory effect on peronophythora litchii, and have agood prospect of development and application.

Description

technical field [0001] The invention belongs to the technical field of plant disease biocontrol, and in particular relates to a strain of bacillus alpina XJGS-4 and its application in preventing and treating litchi frost blight. Background technique [0002] Litchi (Litchi chinensis Sonn.) is an important tropical and subtropical fruit planted in southern my country. Its fruit has good color, fragrance, rich nutrition, and has medicinal value when eaten in moderation. It enjoys the reputation of "Chinese precious fruit". One of the most competitive export fruits in our country. However, since the ripening season of litchi is midsummer, it is easy to cause huge economic losses due to browning and rot during picking, transportation and storage, and post-harvest diseases are the main cause of rot. Litchi frost blight is the main disease causing litchi fresh fruit rot in the postharvest stage. However, at present, the control of litchi frost blight in production is mainly based...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 习平根黄桢辉于戈关天放袁玉花孔广辉李敏慧范晓宁姜子德
Owner SOUTH CHINA AGRI UNIV
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