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Biological agent, degradation agent and system for deep degradation of polychlorinated phenol and application of biological agent, degradation agent and system

A technology for polychlorinated phenols and biological agents, which is applied in the field of polychlorinated phenol biodegradation, can solve the problems of loss of activity, gradual degradation of polychlorinated phenols, and secondary hazards, and achieves the effect of increasing tolerance.

Active Publication Date: 2022-08-05
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003]The traditional phenolic wastewater treatment technology does not carry out targeted step-by-step degradation according to the biodegradation characteristics of polychlorinated phenols, but uniformly adopts anaerobic Or aerobic single treatment, which caused polychlorinated phenols to not be deeply and thoroughly degraded, resulting in a certain concentration of chlorinated phenols and derivatives in the treated water
For the degradation of polychlorinated phenols, anaerobic and aerobic alternating processes are occasionally performed, but the disadvantage is that aerobic-anaerobic alternating is carried out in the same container, which causes anaerobic bacteria, especially strictly anaerobic bacteria, to come into contact with the air. It will lose its activity and weaken its ability to dechlorinate chlorophenols in the anaerobic stage, resulting in incomplete degradation in the subsequent oxygenation stage
Therefore, at present, no matter whether it is a single treatment method or an aerobic-anaerobic alternating method, the polychlorinated phenols cannot be completely degraded in depth, but some phenol derivatives are generated by the degradation, which brings some potential hazards to the environment. secondary hazard

Method used

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  • Biological agent, degradation agent and system for deep degradation of polychlorinated phenol and application of biological agent, degradation agent and system
  • Biological agent, degradation agent and system for deep degradation of polychlorinated phenol and application of biological agent, degradation agent and system
  • Biological agent, degradation agent and system for deep degradation of polychlorinated phenol and application of biological agent, degradation agent and system

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Embodiment 1

[0030] A system for the advanced degradation of polychlorinated phenols, see figure 1 , including: an anaerobic degradation device and an aerobic degradation device, the anaerobic degradation device is communicated with the aerobic degradation device; the anaerobic degradation device is equipped with anaerobic domestication liquid, reducing dechlorination biological agent and trichlorophenol; The aerobic degradation device is equipped with an aerobic acclimation liquid, a biodegrading agent and phenol; the anaerobic degradation device includes: an anaerobic acclimation tank 1 and a primary treatment tank 2; the aerobic degradation device includes: an aerobic acclimation tank 3, Secondary treatment tank 4 and tertiary standstill tank 5; wherein, the two inlets of anaerobic acclimation tank 1 are communicated with anaerobic acclimation liquid pool 6 and nitrogen tank 7 respectively, and the outlet is communicated with the inlet of primary treatment tank 2; The inlet of the oxyge...

Embodiment 2

[0036] A biological agent for the deep degradation of polychlorinated phenols, comprising: a reductive dechlorination biological agent and a degrading biological agent; wherein, the reductive dechlorination biological agent comprises, in parts by weight: 1 part of Pseudomonas putida, 1 part of Velez spore 1 part of bacillus, 1 part of activated sludge and 0.01 part of liquid laccase; the biodegrading agent includes, in parts by weight: 1 part of Burkholderia cepacia, 1 part of Candida utilis, 1 part of activated sludge and Liquid Laccase 0.01 part.

[0037] Among them, Pseudomonas putida was purchased from the China General Microorganism Culture Collection and Management Center with the number of CGMCC1.8829, which can be reductively dechlorinated under anaerobic conditions;

[0038] Bacillus velesi is a self-sieving bacteria capable of producing dechlorinating enzymes to dechlorinate polychlorinated phenols. It was deposited in the China Center for Type Culture Collection on...

Embodiment 3

[0041] A degradation agent for deep degradation of polychlorinated phenols, comprising: anaerobic degradation agent and aerobic degradation agent; wherein, the anaerobic degradation agent comprises: anaerobic domestication liquid, reductive dechlorination biological agent and reductive dechlorination inducer ; Aerobic degradation agents include: aerobic acclimation solution, biodegradation agent and ring-opening inducer; anaerobic acclimation solution includes: sucrose 2g / L, sodium lactate 0.6g / L, yeast powder 1g / L, ammonium chloride 0.5g / L L, sodium thiosulfate 1g / L, potassium dihydrogen phosphate 2g / L, magnesium chloride 0.2g / L, calcium chloride 0.02g / L, sodium chloride 0.005g / L, ferric chloride 0.002g / L, chlorine Manganese 0.0005g / L, Cupric Chloride 0.002g / L, Sodium Selenite 0.001g / L, Sodium Molybdate 0.001g / L, Cysteine ​​0.5g / L, Vitamin B 1 0.001g / L, vitamin B 2 0.001g / L, vitamin B 6 0.002g / L, vitamin B 9 0.00005g / L, vitamin B 12 0.0000025g / L, p-aminobenzoic acid 0.001...

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Abstract

The invention discloses a biological agent, a degradation agent, a system and a method for deeply degrading chlorophenol. The biological agent comprises an anaerobic reductive dechlorination biological agent and an aerobic degradation biological agent, wherein the anaerobic reductive dechlorination biological agent is prepared from the following components in parts by weight: 1 part of pseudomonas putida, 1 part of bacillus velezensis, 1 part of activated sludge and 0.01 part of liquid laccase; the aerobic degradation biological agent is prepared from the following components in parts by weight: 1 part of burkholderia cepacia, 1 part of candida utilis, 1 part of activated sludge and 0.01 part of liquid laccase; the degradation agent comprises an anaerobic degradation agent and an aerobic degradation agent; the system comprises an anaerobic degradation device and an aerobic degradation device. The anaerobic dechlorination treatment device and the aerobic ring opening treatment device are adopted according to the types of the polychlorinated phenols in the wastewater, and specifically domesticated microbial populations and biological agents are added respectively, so that the tolerance of microorganisms and the degradation efficiency of the polychlorinated phenols are improved.

Description

technical field [0001] The invention relates to the technical field of polychlorinated phenol biodegradation, and more particularly to a system and method for deep biodegradation of polychlorinated phenol. Background technique [0002] Chlorophenols are organochlorine compounds with poor degradability and high pollution formed by the substitution of phenolic hydrogen atoms by chlorine. They are mainly found in industrial environments such as coal, petrochemicals, and papermaking. Insecticides are widely used in agriculture, causing serious pollution of soil and groundwater worldwide, and have been listed as priority pollutants by the US Environmental Protection Agency. Biodegradation is the main method to remove chlorophenols in the environment. Low-chlorophenols (monochlorophenol, dichlorophenol) have higher degradability and can be directly degraded by aerobic microorganisms. Compared with low-chlorophenols, polychlorophenols (three The biodegradability of chlorophenol, t...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/30C02F101/34C02F101/36
CPCC02F3/34C02F3/342C02F3/30C02F2101/345C02F2101/36Y02W10/10
Inventor 马英辉卢美欢仝泽方王燕李利军
Owner MICROBIOLOGY INST OF SHAANXI
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