An irrigation method for continuous restoration of soil microbial flora in continuous cropping of melon crops
A technology of soil microorganisms and biological inoculants is applied in the field of irrigation for continuous restoration of the continuous cropping soil microbial flora of melon crops. Sustainable control, simplified water and fertilizer infiltration system, great social and ecological benefits
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Embodiment 1
[0028] Embodiment 1 is used to repair the irrigation method of melon crop continuous cropping soil microbial flora
[0029] (1) Preparation of biological bacteria agent
[0030] The biological agent is the fermentation liquid of Burkholderia phytofirmans, the Burkholderia phytofirmans, depository unit: China Microbial Cultures Preservation Committee General Microbiology Center, address is Beichen West, Chaoyang District, Beijing Road No. 1 Yard No. 3, Institute of Microbiology, Chinese Academy of Sciences, the preservation number is CGMCC No.15326, the strain number is 20180101Liao CK1, and the preservation date is January 29, 2018;
[0031] The formula of the biological agent culture medium includes: tryptone 5g / L, yeast extract 5g / L, seaweed fertilizer powder 10-30g / L, cornstarch 2-5g / L, adjust the pH to neutral; the culture medium is sterilized Finally, inoculate Burkholderia and carry out fermentation, the fermentation temperature is 28 ℃, the stirring speed is 120rpm, an...
Embodiment 2
[0040] Greenhouse field application test
[0041] (1) Test time: 2018.
[0042] (2) Experimental crop: muskmelon, variety: Pengshuai Tianwang (Cangzhou Chunfeng Seed Industry Co., Ltd., Hebei).
[0043] (3) Continuous cropping period: 3 years.
[0044] (4) Processing 1 adopts embodiment 1 to be used for repairing the irrigation method of melon crop continuous cropping soil microbial flora, and biological bacteria Burkholderia concentration 1 * 10 in the described irrigation water and fertilizer 2 -1.0×10 3 cfu / ml, seaweed fertilizer 0.001-0.008g / L; treatment 2 is the control normal irrigation water, and the test period is the whole growing season of the crops.
[0045] (5) The rest are cultivated and managed normally.
[0046] (6) Effects of irrigation methods used to repair the microbial flora of continuous cropping soil of melon crops on the content of phenolic acids in continuous cropping soil
[0047] One month, two months, three months, and four months after the melo...
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