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Burkholderia and application thereof

A technology of Burkholderia and Kkholderia, applied in the field of microorganisms, to achieve the effects of promoting absorption, increasing growth height, and increasing growth speed

Active Publication Date: 2021-07-23
ANHUI QIMEN COUNTY QIHONG TEA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tea polyphenols, also known as "tea tannins" or "tea tannins", are an important biologically active component in tea. Tea polyphenols in black tea are important precursors of theaflavins and the main indicator for improving the quality of black tea. There are no reports on the increase of total tea polyphenols by Burkholder strains

Method used

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  • Burkholderia and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A Burkholderia XYQH-1, the strain has been preserved in the China Center for Type Culture Collection on January 18, 2021, and the preservation number of the Burkholderia XYQH-1 is: CCTCC M 2021105 XYQH-1.

[0031] Among them, other reagents used in the examples of the present invention were all analytically pure.

[0032] The Burkholderia XYQH-1 preserved in the present invention is isolated from the rhizosphere soil of the tea garden in Ruokeng Town, Qimen County, Huangshan City, Anhui Province. Specific steps are as follows:

[0033] 1) Screening of bacterial strains: Weigh 10.0 g of fresh soil samples, place them in a 250 mL Erlenmeyer flask filled with 90 mL of sterile water, and place them at 28-30°C, 180 r min -1Shake in a full-temperature shaking incubator for 30 min, mix well to make a soil suspension, take 100 μL of the soil suspension and add it to a 1.5 mL sterilized centrifuge tube filled with 900 μL of sterile water, and use a ten-fold gradient dilution m...

Embodiment 2

[0052] A growth-promoting application of Burkholderia XYQH-1 in tea tree cultivation, specifically as follows:

[0053] On June 1, 2020, bacteria were applied to the current year's tea seedlings (China Tea 108). The plant height of the tea seedlings was measured on August 1, 2020, two months after the tea seedlings were potted with the strain culture solution.

[0054] 1) Activation: Take Burkholderia XYQH-1 preserved in glycerin, shake the glycerol tube up and down to distribute the bacteria solution and glycerin evenly, then use a burnt and cooled inoculation loop to dip the bacteria solution in the glycerin tube, and put The inoculation loop dipped in the bacterial solution was streaked on the LB solid medium for activation, and then the LB solid plate of the activated strain was sealed with parafilm and the strain name and date were marked with a marker pen. Wherein, the formula of the LB solid medium is peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar ...

Embodiment 3

[0060] The isolated Burkholderia XYQH-1 of the present invention can also increase the total amount of tea polyphenols in tea tree leaves, and the potted treatment of tea seedlings specifically includes the following steps:

[0061] 1) Activation: Take Burkholderia XYQH-1 preserved in glycerin, shake the glycerol tube up and down to make the bacteria liquid and glycerin evenly distributed, then use a burnt and cooled inoculation loop to dip the bacteria liquid in the glycerin tube, and put The inoculation loop dipped in the bacterial solution was streaked on the LB solid medium for activation, and then the LB solid plate of the activated strain was sealed with parafilm and the strain name and date were marked with a marker pen. The formula of LB solid medium is: peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar powder 20g, pH 7.0-7.2.

[0062] 2) Cultivation: Place the marked activated strain sealed plate upside down and place it in a constant temperature in...

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Abstract

The invention provides Burkholderia and application, and belongs to the technical field of microbial strains. The Burkholderia XYQH-1 is preserved in the CCTCC (China Center for Type Culture Collection), and the preservation number is CCTCC M 2021105. After a culture solution of the strain is inoculated to rhizosphere soil of root parts of potted tea seedlings, the growth of the tea seedlings can be promoted, the total amount of tea polyphenols is increased, and the fermentation conditions of the Burkholderia XYQH-1 are easy to operate. Meanwhile, the Burkholderia XYQH-1 can also be used for preparing a natural microbial fertilizer, can promote the growth of tea trees and increase the content of tea polyphenols, provides an alternative for the tea market, belongs to a novel environment-friendly fertilizer, and can greatly reduce the environmental pollution caused by chemical fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Burkholderia bacterium and an application thereof. Background technique [0002] Burkholderia belongs to the genus Burkholderia. It is a bacterium that widely exists in water, soil, plants and human body. The isolated colony is cultured on the potassium solution medium at 28°C for 3-4 days, with a diameter of 8-13 mm. After being activated or purified in LB medium for 24 hours, the diameter of the colony is about 8-10 mm, and the diameter of the colony after 3 days of cultivation Up to 15-20 mm. [0003] Tea polyphenols, also known as "tea tannins" or "tea tannins", are an important biologically active component in tea. Tea polyphenols in black tea are important precursors of theaflavins and the main indicator for improving the quality of black tea. There is no report on the increase of total polyphenols in tea leaves by Burkholder strains. The patent scr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 张显晨王宁宁姜红吴锡端
Owner ANHUI QIMEN COUNTY QIHONG TEA
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