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Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called alanine aminotransferase (ALAT) and was formerly called serum glutamate-pyruvate transaminase (SGPT) or serum glutamic-pyruvic transaminase (SGPT) and was first characterized in the mid-1950s by Arthur Karmen and colleagues. ALT is found in plasma and in various body tissues but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
Preparation method and application of a glutamic-pyruvic transaminase biosensor
The application belongs to the technical field of biomedical detection and electrochemical sensor, and relates to a preparation method and application of a glutamate-pyruvate transaminase biosensor. A soluble metal salt containing cobalt cyanide ions is added into deionized water; copper salt and a complexing agent are uniformly dispersed with deionized water, and after mixing, a complexing reaction is carried out, and then filtration is carried out; the obtained solid is washed and dried, and then is uniformly mixed with polyvinylpyrrolidone, centrifuged, calcined, and CuCo / C nanoparticles are obtained, which are fixed on a gold electrode to obtain a working electrode. Glutamate oxidase, L-alanine and alpha-ketoglutaric acid are uniformly mixed with chitosan to obtain an enzyme solution, and a glutamate-pyruvate transaminase sensor is obtained by dropwise addition. The glutamate-pyruvate transaminase biosensor prepared by the application has a wide linear range and good sensitivity.
Owner:NANJING TECH UNIV