Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model

A technology of phosphorylated muramic acid dipeptide and animal models, which can be applied to dipeptide components, pharmaceutical formulations, organic active ingredients, etc., and can solve problems such as inability to fully reflect immune fulminant liver damage

Active Publication Date: 2020-01-07
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing models are still unable to fully reflect the mechanism of immune fulminant liver injury. ...

Method used

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  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model
  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model
  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model

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Experimental program
Comparison scheme
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Embodiment 1

[0035] 1. Animal model of fulminant liver injury constructed by L18-MDP combined with D-GalN:

[0036] Reagents used: the ligand of the intracellular pattern recognition receptor NOD2 is MDP, the degradation product of peptidoglycan on the bacterial cell wall, and its artificially synthesized phosphorylated derivative is L18-MDP, which is purchased from Invivogen Company, product number tlrl-lmdp. In addition, the D-GalN used was purchased from sigma company, item number G0500.

[0037] (1) Female C57BL / 6 mice, 4-6 weeks old, weighing about 14-15g, divided into 4 groups, including blank control group, L18-MDP treatment group, D-GalN treatment group, L18-MDP combined with D-GalN treatment group . Among them, L18-MDP was injected into the tail vein (10ug / mouse), and D-GalN was injected intraperitoneally (20mg / mouse). Fresh eye blood and mouse liver, lung, kidney, small intestine and large intestine samples were collected 16 hours after injection for subsequent analysis.

[003...

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Abstract

The invention relates to the technical field of animal model construction, in particular to application of phosphorylated muramyl dipeptide for preparing a medicine capable of constructing fulminant liver injury animal model. D-galactosamine and the phosphorylated muramyl dipeptide are adopted for jointly constructing the fulminant liver injury animal model, an animal serum ALT (Alanine transaminase) level can be induced to sharply rise under a situation that a proper dosage is given, and hepatic cells can be protected from massive necrosis. Since an injury phenomenon happens in short time after injection, a determination result shows that the fulminant liver injury animal model is successfully constructed.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to the application of phosphorylated muramic acid dipeptide in the preparation of drugs for constructing animal models of fulminant liver injury. Background technique [0002] Clinically, fulminant liver injury is a life-threatening emergency. Studies have shown that liver tissue and cell damage mediated by the body's immune response is involved in the occurrence and development of fulminant liver injury, but the specific mechanism remains to be further studied. Liver injury caused by different etiologies has different relatively specific immune response characteristics. Establishing an ideal animal model of immune fulminant liver injury is one of the necessary conditions for studying the mechanisms of various immune liver injuries. [0003] NOD2 is a pattern recognition receptor that mainly exists in cells. In recent years, attention to NOD2 has been increasing. ...

Claims

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Application Information

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IPC IPC(8): A61K38/05A61K31/7008A01K67/027
CPCA61K38/05A61K31/7008A01K67/027A01K2267/03
Inventor 殷文伟蔡英任红
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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