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New application of rotigotine in preparation of acute liver injury resisting drug

A technology for acute liver injury and liver injury, applied in the field of pharmacy, which can solve the problems of undisclosed information about rotigotine’s anti-acute liver injury, and achieve the goal of reducing liver tissue pathological damage, reducing concentration, and reducing diffuse hemorrhage in liver tissue Effect

Inactive Publication Date: 2021-03-30
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But the prior art does not disclose any information that rotigotine has anti-acute liver injury

Method used

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  • New application of rotigotine in preparation of acute liver injury resisting drug
  • New application of rotigotine in preparation of acute liver injury resisting drug
  • New application of rotigotine in preparation of acute liver injury resisting drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 LPS / D-Gal-induced acute liver injury experiment in mice

[0021] Materials and Reagents

[0022] Rotigotine (Sigma-Aldrich, USA); lipopolysaccharide (Sigma-Aldrich, USA); D-galactosamine (Sigma-Aldrich, USA); alanine aminotransferase (ALT) test kit (Nanjing Jiancheng Institute of Bioengineering ); Aspartate Transaminase (AST) Test Kit (Nanjing Jiancheng Bioengineering Institute); Mouse Tumor Necrosis Factor-α (TNF-α) Kit (Beijing Suo Laibao Co., Ltd.).

[0023] Animals: SPF grade BALB / c mice, male, weighing 18-22 g, provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd., certificate number: SCXK (Lu) 20190003.

[0024] Instruments: electronic balance (Shanghai Mettler Optiphot Company); cryogenic centrifuge (Shanghai Eppendorf Company); incubator (Shanghai Changliu Scientific Instrument Co., Ltd); SpectraMax M3 multi-functional microplate reader (Molecular Devices, USA).

[0025] 1) The experimental steps of acute liver injury induced by LPS / D-Gal ...

Embodiment 2

[0032] 1) Serum AST and ALT activity detection: 6 hours after injecting LPS / D-Gal, take the mice in Example 1, take blood from the inner canthus vein of the mouse eyes, let stand at room temperature for 0.5 hours, 4°C, 3500 r / min, Centrifuge for 15 min. Transfer the supernatant serum to a new EP tube. Serum AST and ALT activities were detected by alanine aminotransferase (ALT) test kit and aspartate aminotransferase (AST) test kit, respectively. The results are expressed as mean ± standard deviation (n = 8).

[0033] Test results such as figure 2 and image 3 As shown, compared with the control group, the serum AST and ALT activities of the mice in the model group were significantly increased ( ** P # P ## P <0.01).

[0034] figure 2 and image 3 The results showed that the injection of rotigotine before the injection of LPS / D-Gal in mice could reduce the activities of serum AST and ALT in mice with acute liver injury induced by LPS / D-Gal.

[0035]2) Detection of ...

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PUM

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Abstract

The invention relates to the field of pharmacy, discloses a new application of rotigotine in preparation of an acute liver injury resisting medicine, and particularly discloses an application of rotigotine in preparation of the acute liver injury resisting medicine. Rotigotine can reduce the levels of alanine transaminase (ALT) and glutamic oxalacetic transaminase (AST) in serum of mice with liverinjury induced by lipopolysaccharide (LPS) / D-Gal, reduce the concentration of TNF-alpha in serum of mice with acute liver injury, relieve pathological injury of liver tissues and reduce diffuse hemorrhage of the liver tissues. Pharmacological experiments prove that rotigotine has the effect of relieving acute liver injury, and a new prevention or treatment drug is provided for liver injury.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to the new application of rotigotine in the preparation of anti-acute liver injury medicine. Background technique [0002] Liver injury mainly refers to the damage of liver tissue. A variety of factors can cause liver damage, such as viral infection, excessive intake of ethanol, improper medication, exposure to chemical poisons, ischemia-reperfusion, radiation injury, etc. Acute liver injury refers to the rapid occurrence of liver cell damage and abnormal liver function within a relatively short period of time. Severe or persistent liver damage that cannot be effectively controlled in time will eventually lead to liver failure. Various inflammatory mediators and pro-inflammatory cytokines released during the development of liver injury can also cause systemic inflammatory responses, which in turn involve extrahepatic organs. [0003] Rotigotine is a non-ergot alkaloid dopamine receptor ag...

Claims

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Application Information

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IPC IPC(8): A61K31/381A61P1/16
CPCA61K9/0019A61K31/381A61P1/16
Inventor 岳淑敏王天杨韵琦
Owner YANTAI UNIV
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