93 results about "Geobacillus stearothermophilus" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses a straw-decomposing inoculant which comprises the following active components: bacillus subtilis, aspergillus niger, trichoderma viride, phanerochaete chrysosporimn and geobacillus stearothermophilus. In the straw-decomposing inoculant, the total content of effective viable bacteria is (1-2)* 10<8>/ml. With adoption of the straw-decomposing inoculant, the fertilizer prepared from straws can be rapidly degraded, and the normal compost fermentation is performed in advance for at least 15 days. When the straw organic fertilizer prepared from the straw-decomposing inoculant is used as the base fertilizer, alkali-hydrolyzale nitrogen, rapid available phosphorus, potassium and organic contents in the soil are higher than that in the soil in which straws are directly utilized and no fertilizer is applied, and the urease activity and cellulase activity in the soil are obviously higher than that in the soil in which straws are directly utilized and no fertilizer is applied.

The invention relates to a coding gene of heat-resistant type pullulanase from geobacillus stearothermophilus as well as recombinant expression thereof and application thereof. The coding gene successfully constructs a recombinant plasmid containing PluGS, so that various highly-expressed bacterial stains which comprise escherichia coli, bacillus, saccharomycete and mycete are obtained. A recombinant host cell is suitable for expressing a gene of the heat-resistant type pullulanase. The optimal pH value of pullulanase is 6.0, and relatively high enzyme activity is kept when the pH value is 5.0-7.0. The optimal temperature is 65 DEG C, thermal stability is good at a temperature of 60-70 DEG C, the heat is preserved for 3 hours at the temperature of 60 DEG C, and the pullulanase still keep 90% or more of enzyme activity, and is suitable for industrial application needs. The pullulanase which is in recombinant expression is identified as I type pullulanase through thin-layer chromatography and efficient liquid-phase chromatographic analysis, can be used for hydrolyzing pulullan, amylopectin and alphla-1,6-glucosidic bond of soluble starch, and can not hydrolyze amylopectin and cyclodextrin.

The invention discloses a high-temperature kitchen waste degradation microbial agent and a preparing and using method thereof. A microbial strain comprises, by weight, 15-30 parts of nuby land anoxybacillus, 10-25 parts of liquefaction thermophilic bacillus, 20-40 parts of hot nitrogen bacillus and 25-50 parts of geobacillus stearothermophilus. Compared with the prior art, the high-temperature kitchen waste degradation microbial agent has the beneficial effects that as for kitchen waste with different integrants, reasonable matching on single bacterium powder can be carried out, and single bacterial strains are cooperated for degrading the kitchen waste in a collaborative mode; the number of effective viable bacteria in the single bacterium powder in the composite microbial agent is large, and when the microbial agent is used for degrading the kitchen waste, only a small number of microbial agents is needed for sufficiently and rapidly degrading the kitchen waste; components in the microbial agent are high-temperature cultures, the optimum fermentation temperature ranges from 55 DEG C to 65 DEG C, pathogenic bacteria such as escherichia coli and staphylococcus aureus in the kitchen waste can be effectively inactivated in the fermentation process, and the biosecurity is high.

The invention discloses a biological organic fertilizer. The biological organic fertilizer comprises the following components in parts by weight: 100-1000 parts of straw and 1-15 parts of compound microbial agent, wherein the active components of the compound microbial agent include bacillus subtilis, Aspergillus oryzae, Trichoderma reesei, white rot fungi and Bacillus stearothermophilus. In the biological organic fertilizer disclosed in the invention, the straw is used as the raw material for compost, and the compound microbial agent facilitating the degradation of components including cellulose, hemicellulose and lignin is added to accelerate the decomposition of the straw to prepare the organic straw fertilizer. When the prepared fertilizer is applied to farmlands, the soil property can be improved, and the yield and quality of crops can be increased. The compound microbial agent can quickly degrade the straw to prepare the fertilizer, and the time cost in preparing the fertilizer using the compound microbial agent is shorter than the time cost in normal compost fertilization by at least 15 days.

The embodiments of the present invention provide a composite microbial agent for treating black and odor water bodies, wherein the composite microbial agent is mainly used for treating black and odorwater bodies of urban river courses and town pit ponds, and comprises Pseudomonas sp. AW0H4, Bacillus megaterium AW0F5, Alcaligenes faecalis, Bacillus pumilus and Geobacillus stearothermophilus. According to the present invention, the single strain is obtained through a fixed screening method, wherein the microbial biomass achieves 1*10<9> cfu/mL after the single strain is activated and concentrated, and the composite microbial agent is prepared by mixing according to a ratio of 2:2:2:1:1 or 2:2:1:1:1; the storage method comprises: adding a glycerol protection agent to the single strain concentrated bacterial liquid or the composite microbial agent, and freezing; and the composite microbial agent has high specificity for black and odor water bodies, and the metabolic functions of the strains in the composite microbial agent are highly complementary to each other.

The invention is a compound microbial inoculant that processes human and animal excreta effectively composed of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and thermal amylase streptomycete. The microbial inoculum uses metabolism to decompose human and animal excreta effectively in short period of time effectively without secondary pollution during process, which extremely reduces environmental pollution brought by human and animal excreta. Meanwhile, the method is sample, low in cost, and saves resources, which can be widely applied to fixed or movable rest rooms, matching the policies of sustainable development.

The invention relates to a method for obtaining geobacillus stearothermophilus spores. The method comprises the following steps of inoculating the geobacillus stearothermophilus into a liquid seed culture medium; performing culture to obtain seed liquid; inoculating the seed liquid into a liquid spore producing inducing culture medium to be subjected to induced culture; performing induction to produce the spores. The method has the advantages that the culture of the seed cells and the induction of the spores are both performed in the liquid culture; the culture and induction efficiency are greatly improved; the time is shortened; the labor operation is reduced; the high spore number and the high spore yield can be obtained in 36 to 48 hours.

The present invention relates to a spent mushroom substrate high-temperature decomposition composite bacterial agent, wherein eight thermophilic bacteria such as Aeribacillus pallidus, Geobacillus stearothermophilus, Brevibacillus thermoruber, Clostridium stercorarium subsp. thermolacticum, Clostridium thermocellum, Desulfotomaculum nigrificans and the like are subjected to mixing and mixing culture according to a certain cell number content ratio, composite bacterial agent preparation is performed when decomposition ability is high, different spent mushroom substrates are adopted as absorption carriers, peptone, yeast powder and the like are added, the bacterial liquid is repeatedly sprayed, absorption fermentation is performed, and drying at a temperature of 60 DEG C is performed to obtain the composite bacterial agent. The composite bacterial agent can be used for spent mushroom substrate feeds, spent mushroom substrate fertilizers and energy source conversion and other uses.

The invention relates to the microorganism agent for degrading hogwash garbage and the preparation craft, which is formed by mixing seven active germ powders in certain matching. The seven germs are as following: Bacillus subtilis E4, H11, Bacillus licheniformis A2, I1, Bacillus cereus F3, F6 and Bacillus stearothermophilus H13. The invention resolves the problems of bad degrading result and complicated preparation of the hametz due to overfull germ sorts existing in the import microorganism agent degrading the oil component in the garbage, and adopts bacillocin as the strain to obtain the germ powder through the crafts of seed culturing, fermentation culturing and sponging drying. The invention has the advantages of small sorts of germ, simple fermenting process, high concentration of germ in the germ powder and good degrading result, and can achieve minimization treatment, innocent treatment and treatment of being turned into resources, therefore can not only improve the environment sanitary of the city, but also lay a solid foundation for large-scale commercial process. The invention also has an advantage of good economic benefit.

The invention discloses a quick detection method for antibiotic residue in raw milk and belongs to the technical field of biology. According to the quick detection method disclosed by the invention, frozen, dried and stored geobacillus stearothermophilus is added into nutrient gravy agar culture medium to be activated and cultured; the activated geobacillus stearothermophilus is inoculated into phosphate buffer solution to obtain suspension liquid; the geobacillus stearothermophilus suspension liquid is added into bromcresol purple peptone culture medium to obtain test culture medium; the test culture medium is evenly mixed with raw milk to be detected according to volume ratio of (1:2) to (1:3) to be cultured in water bath in 75 DEG C to 80 DEG C for 2.0h to 2.2h, and color change of culture medium is observed. According to the quick detection method disclosed by the invention, system color change reaction time is greatly shortened by adjusting water bath culture temperature to 75 DEG C and coordinating corresponding reasonable volume ratio of the test culture medium to the raw milk, the time is shortened by about 20%, and detecting efficiency is further improved.

The invention discloses a special liquid microorganism fertilizer capable of preventing hardening and promoting growth for wheat. The special liquid microorganism fertilizer is prepared from the following components in parts by weight: 20 to 50 parts of nitrogen fertilizers, 30 to 40 parts of phosphate fertilizers, 10 to 30 parts of potassium sulfate, 5 to 8 parts of boric acid, 3 to 7 parts of zinc sulfate, 2 to 5 parts of ammonium molybdate, 3 to 6 parts of ethylene diamine tetraacetic acid (EDTA) iron chelate, 4 to 5 parts of EDTA manganese chelate, 10 to 12 parts of chitosan, 10 to 15 parts of amino acids, 5 to 6 parts of fulvic acid, 1 to 2 parts of sodium polyacrylate, 20 to 30 parts of microbial agents, 15 to 25 parts of traditional Chinese medicine extraction solutions, and 1000 parts of deionized water. The microbial agents are composite microbial agents comprising 20 to 30 percent of bacillus subtilis, 15 to 25 percent of bacillus laterosporus, 18 to 25 percent of bacillus amyloliquefaciens, 10 to 20 percent of geobacillus stearothermophilus and 12 to 18 percent of bacillus mucilaginosus. The traditional Chinese medicine extraction solutions are obtained by mixing radix sophorae flavescentis, herba artemisiae annuae, folium artemisiae argyi, flos lonicerae, rhizoma polygoni cuspidati, northeastern radix gentianae, rhizoma atractylodis, radix cynanchi paniculati extracts and the like in proportions. According to the fertilizer disclosed by the invention, disease-resistance and disease-prevention abilities of the wheat are improved, and the soil is improved.

The invention relates to a microbial degradation method of coal waste, which comprises the following steps: coal slime is dried and then crushed; mushroom residue, yeast peptone and rice bran are added and uniformly mixed, and a compound microbial agent of Bacillus stearothermophilus, Bacillus mucilaginosus, Bacillus subtilis, Bacillus amyloliquefaciens, Streptomyces, Trichoderma and Pseudomonas fluorescens according to the weight ratio is added; after mixing, the stacking is in a long strip shape, when the temperature on the position below the stacking surface by 30 cm decreases to below 35 DEG C, the stacking is turned over 48-72 hours per time, and the fermentation time is 28-45 days. The invention is beneficial to promoting the utilization of coal slime resources, effectively utilizingsolid waste generated in the process of coal processing and use and in agricultural production and living, and is beneficial to environmental protection. And the method makes full use of the rare mineral elements and humic acid in coal slime to serve the revitalization of rural areas and the sustainable environment of green agriculture.

The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.

The invention provides a novel biological organic fertilizer. Raw materials of the novel biological organic fertilizer comprise saw powder, cow manure, chicken manure, domestic garbage, geobacillus stearothermophilus additives, lactobacillus plantarum additives, traditional Chinese medicine additives and seaweed. The biological organic fertilizer has the noticeable advantages of being safe and capable of improving quality of crops, avoiding pollution, increasing yield of the crops, protecting the soil and reducing usage amount of chemical fertilizers and pesticides,.

The invention relates to a geobacillus stearothermophilus strain. The geobacillus stearothermophilus R2D2-6. 626069934 is preserved in CGMCC ( China General Microbiological Culture Collection Center)and has the preservation number of CGMCC NO.15478. The strain can be used for maintaining the activity and realizing mass breeding in the compost fermentation high-temperature period; the fast decomposition of organic matters in the compost fermentation can be promoted; the time required by the composting can be greatly reduced; the complete composting degree is improved; the loss of nitrogen elements caused by high temperature or time factors can be reduced; the national NY525-2012 organic fertilizer industry standard can be reached. The organic waste resource reutilization in poultry and livestock culture industry can be favorably improved; the environment damage of the poultry and livestock culture industry can be greatly reduced; the geobacillus stearothermophilus strain also conformsto the operation rule of rural revitalization and green environment agriculture sustainable environment.

The invention relates to the technical field of microbe applications and in particular relates to a microbial agent, a greening waste soil remediation agent as well as a preparation method and application thereof. Each milliliter of the agent comprises 1*10<10-12> trichoderma asperellum WL spores, 1*10<10-12> geobacillus stearothermophilus viable spores and 1*10<10-12> CFU bacillus amyloliquefaciens. The agent comprises the trichoderma asperellum WL separated by the inventor, and the strain has an effect of enriching heavy metal materials, has a cellulose degradation function and can be well used for greening waste degradation and soil remediation. The invention further provides a greening waste soil remediation agent. The greening waste soil remediation agent can achieve the effects of overcoming the absorption and digestion difficulty of the conventional urban greening wastes, remediating salt and alkali lands and removing heavy metals and petrochemical pollution sources in contaminated lands.

The invention provides a seaweed biological bacterial fertilizer with a pesticide effect and a fertilizer effect and a production method thereof. The seaweed biological bacterial fertilizer is characterized by being prepared from, by weight, 100-700 parts seaweed residues, 50-300 parts of microbial agent and 200-500 parts of humic acid powder. The seaweed residues are prepared from gulfweed, and gulfweed residues obtained after alginic acid extraction is subjected to low-temperature fermentation; the microbial agent is mixed fermentation liquor of geobacillus stearothermophilus and bacillus amyloliquefaciens; the humic acid powder is a high-activity cell activating agent, and is micromolecular humic acid with the fulvic acid content larger than 50%. The function of microorganisms is high, and the pesticide effect and the fertilizer effect can be achieved. According to the seaweed biological bacterial fertilizer, the production process is simplified, the method that the mixed materials are directly bagged and fermenting and composting continue to be carried out in bags is adopted, the labor intensity and the labor cost are reduced, and then the production cost is reduced.

The invention discloses a method for measuring the number of initial spores of bacillus stearothermophilus in a biological indicator. The method comprises the following steps: adding aseptic water into the biological indicator to obtain a primary diluted liquid, and respectively adding the primary diluted liquid to a plurality of aseptic test tubes; performing thermal shock in a water bath; taking out the aseptic test tubes, quickly cooling to the room temperature, and performing operations on each aseptic test tube as follows: diluting the primary diluted liquid until the primary diluted liquid is in the proper gradient stage by stage by adopting a tenfold increasing dilution method to obtain a secondary diluted liquid, injecting two parts of the secondary diluted liquids into two aseptic plates respectively, injecting soybean casein agar mediums respectively, solidifying, and culturing; counting the numbers of the initial spores of the bacillus stearothermophilus in all the aseptic plates, averagely calculating all counting results, and dividing by the dilution level corresponding to the proper gradient to obtain an initial spore number so as to finish the measurement. The method is stable and feasible through verification, solves the problem of incapability of measuring the number of initial spores of bacillus stearothermophilus in a biological indicator in the past, and is convenient to popularize and apply.