93 results about "Geobacillus stearothermophilus" patented technology

The invention discloses an XZ-A26 bacterial strain for producing L-alanine with high yield, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4036 and has the capacity of generating high-concentration L-alanine through fermentation. The XZ-A26 bacterial strain is constructed by the steps of: integrating an L-alanine dehydrogenase gene on thermophilic fatty bacillus chromosome on lactic dehydrogenase position on an escherichia coli ATCC8739 chromosome, then sequentially knocking out a pyruvate formate lyase gene, an alcohol dehydrogenase gene, an acetokinase gene, a fumaric acid reductase gene and an alanine racemase gene of the escherichia coli chromosome, and then carrying out continuous cell culture in a fermenting tank for obtaining agenetic engineering strain. The invention also relates to a construction method of the XZ-A26 bacterial strain and an application of the XZ-A26 bacterial strain in preparation of the L-alanine. According to the invention, the escherichia coli with the preservation number of CGMCC No.4036 for generating the high-concentration L-alanine through fermentation can be constructed by using a metabolic engineering method, and the yield of the L-alanine generated by using the XZ-A26 bacterial strain reaches up to 115g / L. The XZ-A26 bacterial strain is suitable for industrially producing the L-alanine.

The invention relates to an isolated polynucleotide sequence from the genome of Bacillus stearothermophilus (Geobacillus stearothermophilus) and an amino acid sequence encoded by the polynucleotide sequence, the corresponding amino acid sequence comprising a novel enzyme, Tirt (thermostable intron reverse transcriptase), having reverse transcriptase activity and retaining that activity at temperatures of up to about 75° C.

The invention discloses a preparation method of a bioorganic fertilizer. The method is characterized by adopting a complex microorganism bacterium agent formed by bacillus subtilis, aspergillus oryzae, trichoderma reesei, phanerochaete chrysosporium and geobacillus stearothermophilus as a decomposition agent to degrade straws to prepare the bioorganic fertilizer. The preparation method has the beneficial effects that the strain composition of the complex microorganism bacterium agent is new flora composition, which takes full advantage of the physiological activities of strains to generate cellulose, hemicellulose and biological enzymes, such as ligninase, to accelerate the decomposition process of straws, thus preparing the bioorganic fertilizer; the organic straw fertilizer prepared by the preparation method is used as a base fertilizer, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

The present invention relates to a Geobacillus caldoxylosilyticus strain producing cyclodextrin glycosyltransferase, wherein the strain is Geobacillus caldoxylosilyticus CHB1, is preserved in China Center for Type Culture Collection on August 28, 2013, and has the preservation number of CCTCC M2013384, and it is proved that the enzyme produced by the Geobacillus caldoxylosilyticus is cyclodextrin glycosyltransferase (short for CGTase) belonging to the alpha-amylase family, and can be adopted to catalyze soluble starch so as to generate alpha-cyclodextrin. According to the present invention, the new strain Geobacillussp CHB1 producing CGTase is provided, the optimum enzyme activity reaction temperature of the enzyme production fermentation broth supernatant of the strain is 65 DEG C, the activity is maintained between 40-85 DEG C, and the CGTase produced by the strain can be used for producing alpha-cyclodextrin, and can further be used for processing processes of starch in the temperature range and the like.

The invention particularly relates to a compound fermentation agent in an ectopic fermentation treatment system of livestock and poultry manure. The compound microbial agent for treating manure by ectopic fermentation is composed of the following strains: Geobacillus toebii, Geobacillus stearothermophilus, Aeribacillus pallidus, Psychrobacter faecalis and Geobacillus thermodenitrificans. The compound agent is applied to an ectopic fermentation bed and has the advantages of rapid low-temperature start property, high fermentation temperature, high manure decomposition speed, large water volatilization amount and the like, and defects of low manure decomposition efficiency and high padding consumption rate of an existing microbial agent are overcome.