Microbial degradation method of coal waste and application thereof
A technology of microbial degradation and waste, which is applied in the field of microbial degradation of coal waste, can solve the problem that coal slime is not fully and rationally utilized, and achieve the effect of improving resource utilization
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Embodiment 1
[0049] Biological agent amplification, comprising the following steps:
[0050] Each strain is amplified in the enrichment culture solution to obtain a liquid mother solution. Add sugar, pure water, yeast peptone, and maltodextrin to each microbial strain, respectively, and undergo amplification and fermentation at 45°C for 48 hours. The biological treatment bacteria are separately fermented and amplified, and then mixed with the adsorption carrier to obtain a separate solid microbial agent. Preferably, the adsorption carrier is rice bran.
[0051] The obtained solid bacterial agent was cultivated on behalf of Trilobite Agricultural Technology (Xiamen) Co., Ltd., wherein Geobacillus stearothermophilus (Geobacillus stearothermophilus) R2D2-6.626069934 had an effective viable count of 25 billion / gram, Bacillus colloidus (Bacillus stearothermophilus) mucilaginosus) is 40 billion / gram, Bacillus subtilis is 20 billion / gram, Bacillus amyloliquefaciens is 10 billion / gram, Streptomyces...
Embodiment 2
[0053] The biological treatment steps of coal slime are as follows:
[0054] The coal slime used comes from Shandong Zaozhuang Gas Fertilizer Coal. The coal slime used has an ash content of 55%, a sulfur content of 1.6-1.8, a moisture content of 12-13%, and a calorific value of 2500-2600 kcal, which is easy to form lumps. Such as figure 1 As shown, the display picture of the original coal slime delivered to the factory area before fermentation.
[0055] Check indicators before use.
[0056] The coal slime was sun-dried and pulverized to 10 mesh. After fermentation, it is crushed to 20-30 mesh.
[0057] Add mushroom slag, yeast peptone, and rice bran to the coal slime in the following amounts: coal slime: mushroom slag: yeast peptone: rice bran = 1000:1000:100:2.5, add water to keep the moisture content at 50-60%.
[0058] Then each solid bacterial agent prepared in Example 1 is added in proportion, and the addition amount is by mass ratio, coal slime: Geobacillus stearothe...
Embodiment 3
[0063] The coal slime used comes from the ash from the coal chemical gasification furnace of Ningxia Shenhua Group. The coal content is about 22% and the water content is 6%. It has a porous structure similar to activated carbon.
[0064] Check indicators before use.
[0065] The coal slime is sun-dried and crushed to 10 meshes, and then crushed to 20-30 meshes after fermentation.
[0066] Add mushroom slag, yeast peptone, and rice bran to the coal slime in the following amounts: coal slime: mushroom slag: yeast peptone: rice bran = 1000:1000:100:2.5, add water to keep the moisture content at 50-60%
[0067] Then each solid bacterial agent prepared in Example 1 is added in proportion, and the addition is, coal slime: Geobacillus stearothermophilus: Bacillus colloidus: Bacillus subtilis: Bacillus amyloliquefaciens: Streptomyces: wood Mold: Pseudomonas fluorescens = 1000:1.8:0.8:0.25:0.6:0.3:0.1:0.1.
[0068] Put it in at one time and stir evenly, and the stack is in a long st...
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