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A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof

A technology of composite microorganisms and microbial preparations, which is applied in the direction of organic fertilizers and bacteria, can solve the problems of restricting microbial fertilizers, the difficulty of survival and reproduction of target bacteria, and achieve good storage stability of living bacteria, and enhance plant resistance to diseases and insect pests. Drought resistance and the effect of improving soil fertility

Inactive Publication Date: 2008-10-29
SHANGHAI SIJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the selected strains cannot carry each other or antagonize each other, it will inevitably make it difficult for the target bacteria to survive and reproduce well in the soil, thus seriously restricting the function of microbial fertilizers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation method of organic material decomposing agent:

[0025] Strains: Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Bacillus pumilus, Geobacillus stearothermophilus, Saccharomyces cerevisiae, Streptomyces griseus, Streptomyces salivarius, Pseudomonas alcaligenes, Pseudomonas cepacia , Geotrichum candidum;

[0026] Fermentation culture is carried out by means of liquid submerged fermentation culture; single-strain fermentation can be adopted, and the culture of bacteria has no special regulations on the nutrient source in the culture medium, and carbon sources, nitrogen sources, inorganic salts and Other growth factors; the carbon source can be glucose, sucrose, corn flour, etc., the nitrogen source can be peptone, beef extract, yeast extract, soybean meal powder, etc., and the inorganic salt can be potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sulfuric acid heptahydrate Magnesium, etc., growth factors can be v...

Embodiment 2

[0043] Embodiment 2: the preparation method of organic material decomposing agent:

[0044] Strains: Bacillus licheniformis, Bacillus subtilis, Geobacillus stearothermophilus, Bacillus pumilus, Candida lipolytica, Saccharomyces cerevisiae, Streptomyces griseus, Streptomyces salivarius, Streptomyces thermoamylase, Pseudomonas alcaligenes Monomonas, Aspergillus oryzae, Geotrichum candidum,

[0045] Fermentation and culture of single strains respectively to obtain fermentation liquid, directly mix with adsorbent according to the volume to mass ratio of 30-70%, and dry to obtain single-bacteria solid preparation, then weigh the single-bacteria solid preparation according to the following formula and mix evenly to make a solid Organic material decomposing agent, the number of viable bacteria contained is greater than 2.0×10 8 pcs / g, moisture≤12%, according to mass ratio:

[0046] Bacillus licheniformis solid preparation 0.5~8.0×10 9 pc / g 2~70%

[0047] Bacillus subtilis solid p...

Embodiment 3

[0059] Embodiment 3: the preparation method of liquid microbial inoculant:

[0060] Strains: Bacillus licheniformis, Bacillus megaterium, Bacillus jelly-like, Bacillus lateral sporosum, Azotobacter torus;

[0061] After fermentation and cultivation of a single strain, the fermentation broth is obtained; then the five strains of the fermentation broth are mixed, and then an auxiliary agent is added in a mass-volume ratio. The number of effective viable bacteria is greater than 2.0×10 9 pcs / ml, by volume:

[0062] Bacillus licheniformis liquid 0.5~50.0×10 9 pcs / ml 2~80%

[0063] Bacillus megaterium bacteria liquid 0.5~50.0×10 9 pcs / ml 2~80%

[0064] Jelly-like bacillus liquid 0.1~10.0×10 9 pcs / ml 2~80%

[0065] Bacillus lateralis bacterium liquid 0.1~10.0×10 9 pcs / ml 2~80%

[0066] Azotobacter rotundum bacteria liquid 0.1~10.0×10 9 pcs / ml 2~80%

[0067] Sodium benzoate 0.1% mass volume ratio

[0068] Curcumin 0.1% mass volume ratio.

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Abstract

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

Description

technical field [0001] The invention relates to a group of microbial formulations containing multiple active microorganisms suitable for preparing compound microbial fertilizers and a preparation method thereof. Background technique [0002] The target performance of the strain is unstable, the compatibility is not reasonable, and the stress resistance is poor. The bacterial agent used in microbial fertilizers is a compound bacterial agent composed of target bacteria and auxiliary bacteria. After entering the soil, with the assistance of soil bacteria, it can reproduce stably and eventually form dominant bacteria in the rhizosphere or soil. If the selected strains cannot carry each other or antagonize each other, it will inevitably make it difficult for the target bacteria to survive and reproduce well in the soil, thus seriously restricting the function of microbial fertilizers [0003] Due to the above-mentioned problems and deficiencies in microbial fertilizer products c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08
Inventor 黄循一
Owner SHANGHAI SIJI BIOLOGICAL TECH
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