70 results about "Pseudomonas alcaligenes" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg/L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.

The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.

The invention discloses a waste water treatment agent. The waste water treatment agent is prepared by using the following steps: 1, mixed fermenting, culturing and collecting pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli; 2, mixing polyvinyl alcohol, calcium alginate, acrylyl and water according to a mass ratio of (6-15):(0.5-1.5):(3-5):100 to obtain a polyvinyl alcohol hydrosol, and adding the collected pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli into the polyvinyl alcohol hydrosol to form a mixed solution; 3, adding the mixed solution into a saturated boric acid solution containing N'-methylene bisacrylamide CaSO4 by using a peristaltic pump to form a gel bead, cooling and unfreezing the gel bead, then cooling and unfreezing the gel bead again, placing in phosphate with the mass concentration of 0.2-0.5 g/L to obtain an immobilized cell bead with a spherical appearance and an internal porous mesh structure. The invention also discloses a method for treating waste water by using the treatment agent. Different heavy metals are well adsorbed.

The invention relates to an improved hyposmosis/super-hyposmosis oil extraction sewage reinjection method in an oil field, which is characterized in that a combination strain of Zoogloea sp, Bacillus cereus and Pseudomonas alcaligenes mainly for removing oil and a combination strain of Brachybacteriumcollins Bacillus licheniformis Bacillus subtilis and Pseudomons sp for mainly degrading organic pollutants are respectively attached in an aerobic pool, and suspension filling with density of 65-85V/V% and/or separatly arranged suspension filling and biological aerated filter filling are filled and treated in sequence, wherein a separation membrane is a fiber membrane or vacuum membrane or gas stripping membrane or vibration membrane meeting the requirement on filtration, and the fine filtration is diatomite filtration. The synergistic effect of the process greatly improves the preprocessing effect to ensure that the follow-up membrane separation can adopt conventional economic separation treatment such as the hollow fiber membrane or vacuum membrane or gas stripping membrane or vibration membrane, or adopt the diatomite filtration. Compared with the prior art, the invention has the advantages of short treatment period and low investment and treatment cost.

The invention provides a bacteria strain having a high efficiency broad spectrum degradation ability for an organophosphorus pesticide, the strain belongs to Pseudomonas pseudoalcaligenes, and is named as C2-1, the invention also provides a seperation process, cultivation conditions, and a degradation characteristic of the strain, the strain can be grew on an inorganic salt culture medium by using the pesticide. Also provided is seperation and purification of an organophosphorus pesticide degradation enzyme excreted by the strain, and study on enzyme properties, and seperation and clone of genes coding the enzyme. The invention provides a good gene source for cloning organophosphorus pesticide degradation enzyme genes by using a gene project means, finally realizes an object of producing the organophosphorus pesticide degradation enzyme.

The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU/Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU/Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU/Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU/Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.

The invention relates to kitchen garbage biological fermentation microbial inoculum. The kitchen garbage biological fermentation microbial inoculum consists of four or more of bacillus subtilis, bacillus licheniformis, cellulomonas flavigena, pseudomonas pesodoalcaligenes, rhodop seudanonas palustris, frankiaceae actinomycesbovis, debaryomyces hansenii, and candida tropicalis and is loaded to a carrier consisting of one or two of wheat bran, saw dust or straw, and is subjected to freeze drying to obtain the microbial inoculum; and the microbial inoculum is mixed with kitchen garbage, and a fermentation reaction is performed under 50-65 DEG C, so that the kitchen garbage can be reduced by 70% within 6-10 hours, and the content of generated organic matter exceeds 90%. The kitchen garbage biological fermentation microbial inoculum has the advantages that the technical method can efficiently reduce kitchen garbage, the treated substances can be used for soil modification, and the kitchen garbage biological fermentation microbial inoculum has remarkable economic, social and environmental benefits.

The invention relates to a microbial treatment method for domestic garbage. A biological treatment agent containing bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae is added with water and then is subjected to mixed fermentation with the garbage, so that the objective of degrading the garbage is achieved, and the degradation rate is up to 85%. The method is simple in operation and easy to master, moreover, has good effect on degradation of the garbage, and is beneficial for rapid treatment of the garbage and environmental protection.

The invention provides a preparing method of a compound microorganism agent for papermaking wastewater. The compound microorganism agent is prepared by mixing Trametes versicolor, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus, Pseudomonas alcaligenes and cellulase; the cellulase can decompose celluloses in the wastewater into glucose; compound microorganism can also degrade solids, lignin and the like in the wastewater. The compound microorganism agent is free of chemicals, environment friendly, non-toxic and free of secondary pollution, is simple to use, is insusceptible to environmental factors, has stable effect on wastewater treatment and allows the quality of effluent to meet the national standard.

The present invention relates to a simple, safe, and efficient process for the complete bio-decaffeination of caffeine-containing solutions using fungus Pseudomonas alcaligenes CFR 1708, a method of isolating pseudomonas alcaligenes CFR 1708 useful for the bio-decaffeination of caffeine-containing solutions, a Pseudomonas alcaligenes strain of accession number CFR 1708, and a decaffeinated solution obtained by aforementioned process.

The invention discloses a complex microbial inoculant for a black and odorous water body as well as a preparation method and application thereof. The complex microbial inoculant is obtained by singlyculturing strains such as pseudomonas borbori, pseudomonas alcalophila, geobacillus thermophilus, paracoccus denitrificans and micrococcus lysodeikticus, performing mixed inoculation and fermentation,and then performing collection. The metabolic function of the strains contained in the complex microbial inoculant has high synergetic complementarity; the thallus reproduction rate is high; the production cost is low; the environment protection is achieved; no pollution exits; the performance is excellent and efficient; the water quality of the black and odorous water body can be effectively improved; the COD, NH3-N, TP, and odor degree and chromaticity of the water body can be effectively reduced; the transparency of the water body is improved.

The invention discloses a microbial compound fungicide for degrading waste gas of a rubber plant and a preparation method and application thereof. The compound fungicide comprises, by mass, 5-10 partsof bacillus atrophaeus, 5-10 parts of empedobacter brevis, 2-5 parts of photobacterium beijerinck, 2-5 parts of pseudomonas alcaligenes, 2-5 parts of virgibacillus pantothenticus and 5-10 parts of pseudomonas putida. The microbial compound fungicide can be applied to degradation of the waste gas of the rubber plant, the degradation effect is good, the culture cost is low, the microbial compound fungicide is easy and convenient to use, the VOCs removal rate is 90% or above, and the national emission standard is met.

The invention discloses a complex microbial inoculant for wastewater treatment and a preparation method thereof.The complex microbial inoculant comprises shewanella putrefaciens, pseudomonas pseudoalcaligenes and modified zeolite.Chitosan is introduced to the zeolite, the adsorption performance of the zeolite is improved, then composite modification is conducted, iron phosphate is introduced to the zeolite, and the adsorption performance of the zeolite is improved; the iron ions can be used as a coagulant and cooperate with chitosan to flocculate and settle ammonia nitrogen substances in sewage, the biological nitrification performance of the composite microbial inoculant is greatly improved, in addition, the iron ions can increase the content of organic polymers in bacterial biofilms, and the colloidal network structure of the organic polymers can aggregate cells together, so that the biological nitrification performance of the composite microbial inoculant is improved. Meanwhile, the biological membrane is adhered to the surface of the zeolite carrier, so that the connectivity between the zeolite carrier and microorganisms is increased, the leakage of the microorganisms is effectively prevented, and the complex microbial inoculant can keep a stable biological nitrification effect.

The present invention provides new promoter sequence of Pseudomonas pseudoalcaligenes. The present invention includes gene constituent containing the promoter sequence of the present invention, and the promoter sequence is connected to neomycin phosphotransferase gene sequence encoding the structure gene optionally. The present invention also provides the carrier expressing structure gene encoded product of gene constituent in the present invention and the host cell as well as the cell transformed by the heterologous gene connected to the promoter optionally.

The invention relates to a composite growth promoter. The microbial agent contains Bacillus subtilis, Bacillus amyloliquefaciens, Bordetella sp., Sinorhizobium sp., Pseudomonas alcaligenes, Nitrobacteriaceae, actinomycete, Sporotrichum thermophile, potassium aspartate and gibberellin. The growth promoter provided by the invention can be used for improving agronomic characters of tobacco such as plant height, stem girth, maximum leaf length, maximum leaf width and effective number of leaves; the growth promoter can also be used for improving the accumulation of amount of dry matters of the tobacco in parts such as roots, stems, leaves and the like; and the composite growth promoter provided by the invention can be used for improving the quality of growth of the tobacco.