14 results about "Pseudomonas alcaligenes" patented technology

The invention discloses salt-tolerant pseudomonas alcaligenes and application thereof in improving salt stress resistance of plants, and belongs to the technical field of microorganisms. The pseudomonas alcaligenes are separated from saline-alkali soil, have strong salt tolerance, have a salinity tolerance range of 0-90 g / L, can improve plant salt stress resistance and promote plant growth, and are preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, China, the preservation date is May 19, 2025, and the preservation number is CGMCC No.34593.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

The present invention discloses a composite microbial agent and its preparation method and application. The composite microbial agent comprises Pseudomonas alcaligenes, Citrobacter rodinii and Micrococcus. The preparation method comprises the following steps: culturing Pseudomonas alcaligenes, Citrobacter rodinii and Micrococcus respectively; collecting the bacterial bodies respectively and drying them to prepare Pseudomonas alcaligenes powder, Citrobacter rodinii powder and Micrococcus powder; counting the Pseudomonas alcaligenes powder, Citrobacter rodinii powder and Micrococcus powder respectively, and mixing the Pseudomonas alcaligenes powder, Citrobacter rodinii powder and Micrococcus powder according to the ratio of live bacteria to obtain the composite microbial agent. When the composite microbial agent in the present invention is used to repair the acidified soil in the coal mining area, the pH of the acidified soil can be quickly increased in a short period of time, and the repair effect can be maintained, and the pH of the acidified soil can be continuously increased to neutral, so as to achieve a good soil repair effect.

The invention relates to the technical field of synthesis of lithium battery material ammonium carboxymethyl cellulose, in particular to a preparation method of high-purity battery grade ammonium carboxymethyl cellulose. The preparation method comprises the following steps: S1, pretreatment; s2, preparing a compound bacteria solution, namely mixing the cultured modified trichoderma bacteria solution and pseudomonas alcaligenes bacteria solution according to a volume ratio of 2: 1 to obtain the compound bacteria solution; s3, fermenting and alkalizing; s4, etherification: adding a modified etherifying agent into the alkalized fermentation liquid, heating to 65 DEG C, and reacting for 4-5 hours; s5, carrying out ammoniation; and S6, separation and purification. The invention provides a preparation method of high-purity battery-grade ammonium carboxymethyl cellulose, and aims to solve the problems of large amount of sewage generated in the preparation process of the ammonium carboxymethyl cellulose and serious pollution to the environment in the related technology.

The invention discloses a composition for preventing and treating anthracnose of turpinia arguta and application of the composition, and belongs to the technical field of plant disease prevention and treatment. The composition comprises a microbial agent and a carrier, the microbial agent is prepared from bacillus mycoides, pseudomonas mendocina, pseudomonas alcaligenes and brevibacillus brevis; the carrier comprises algal polysaccharide, chitosan oligosaccharide and calcium carbonate. According to the invention, a prevention and treatment method of treating bacteria with bacteria is adopted, biocontrol bacteria with a prevention and treatment effect on turpinia montana anthracnose are screened, and through scientific and reasonable proportioning and matching of microbial carriers, reproduction of turpinia montana anthracnose germs is effectively inhibited, the morbidity and disease index of turpinia montana anthracnose are reduced, and a new experience method is provided for prevention and treatment of turpinia montana anthracnose.

The present invention relates to the technical field of metal surface treatment, and in particular to a cutting fluid for aluminum alloy and a preparation method thereof. The cutting fluid for aluminum alloy is composed of the following raw materials in weight percentage: 20-25wt% of plant base oil, 10-14wt% of pH buffer, 4-6wt% of lubricant, 2-4wt% of rust inhibitor, 2-3wt% of coupling agent, 0.5-1.0wt% of defoamer, 0.8-1.5wt% of corrosion inhibitor, 6-10wt% of emulsifier, 1-3wt% of antibacterial agent, and the balance is deionized water; the antibacterial agent is an organic guanidine compound and a fluorine-containing baicalein sulfonylated derivative. The organic guanidine compound of the present invention has a bactericidal mechanism: the functional groups of the organic guanidine polymer molecules are positively charged, while bacteria and virus cells in water mostly carry negative charges. After being adsorbed on the surface of microorganisms, the organic guanidine inhibits the division of bacteria and viruses, causing them to lose their reproductive ability, thereby playing an inhibitory and bactericidal role; and the fluorine-containing baicalein sulfonylated derivative has a special binding site with Pseudomonas alcaligenes, thereby having a good inhibitory and killing effect on it.

This invention discloses a strain of *Alcaligenes* HHR-1-1 and its application in the degradation of carotenoids and the preparation of flavorings, relating to the field of microbial fermentation technology. The *Alcaligenes* strain of this invention (… Pseudomonas alcaligenes The HHR-1-1 strain has the accession number CCTCC NO: M 20252745, and the *Alcaligenes* HHR-1-1 strain was deposited at the China Center for Type Culture Collection on December 1, 2025. The *Alcaligenes* HHR-1-1 strain can degrade carotenoids with a degradation rate of 80%–97%, and its main degradation products are β-ionone and its oxides, and dihydroactinolone. Furthermore, the fermentation broth of *Alcaligenes* HHR-1-1 strain and carotenoids can be used in flavor preparation, effectively improving the quality of the flavors.

The present invention discloses a salt-tolerant Pseudomonas alcaligenes strain and its use in improving plant salt stress resistance, belonging to the field of microbial technology. This Pseudomonas alcaligenes strain is isolated from saline-alkali soil and has strong salt tolerance, with a salinity tolerance range of 0-90 g / L. It can also improve plant salt stress resistance and promote plant growth. The strain is deposited with the General Microbiology Center of the China National Center for Microbiological Culture Collection, Beijing, China, on May 19, 2025, under the deposit number CGMCC No. 34593.

The invention belongs to the field of tumor immunity, discloses application of a pseudomonas alcaligenes composition in preparation of pancreatic cancer treatment drugs, and aims to solve the technical problems that pancreatic cancer immunotherapy is low in response rate and existing treatment schemes are high in toxicity. The pseudomonas alcaligenes used in the invention are sPseudomonas alcaligenes, 4-carboxyphenylacetic acid and 1-(4-carboxybutyl) indole-5-boric acid are used as composite cross-linking agents, the composite cross-linking agents and sodium alginate form a hydrogel carrier, the pseudomonas alcaligenes are efficiently encapsulated, a PD-1 antibody is loaded, the tumor weight of the pancreatic cancer is remarkably reduced, tumor growth is effectively inhibited, and the pancreatic cancer can be effectively inhibited. The triple barriers of pancreatic cancer immunotherapy are successfully broken through; the hydrogel carrier is high in bacterial encapsulation efficiency, can effectively construct a growth microenvironment suitable for pseudomonas alcaligenes and promote bacterial growth, and is excellent in biological safety; and a low-toxicity and high-efficiency brand-new technical scheme is provided for immunotherapy of pancreatic cancer.

The application discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS with significant selectivity in inhibiting harmful bacteria in aquaculture is obtained by being isolated and purified from water mould antagonistic actinomycete strain QHV2 for the first time. The polysaccharide polymer QPS can effectively inhibit mycelium growth and spore germination of saprolegnia / branchia mould and destroy mature biofilm. The polysaccharide polymer QPS is stable in activity, harmless to fish fry and good in biological safety, and can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to water when the fermentation product of QHV2 is directly applied, and can selectively inhibit harmful bacteria such as staphylococcus aureus, saprolegnia and branchia mould in aquaculture, and has no inhibition or weak inhibition on beneficial bacteria such as pseudomonas pseudoalcaligenes and bacillus in aquaculture, and has better application effect.

The present invention discloses a method for treating aquaculture wastewater by using a composite bacterium, which includes putting a composite bacterium agent into the aquaculture wastewater and stirring for treatment; wherein, the preparation method of the composite bacterium agent includes inoculating Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella in a liquid medium respectively to obtain bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella; adding porous carbon and the bacterial solutions into a new liquid medium and loading to obtain the composite bacterium agent. When the present invention is used for treatment, it can prevent the loss of enzymes and antibacterial peptides produced by microorganisms, thereby improving the removal rate of ammonia nitrogen in aquaculture wastewater. During the degradation process, part of the nickel is released, improving the activity of microorganisms; the mixing of DL-methionine and the carbon precursor enables good dispersion of nickel species, also increases the specific surface area of the porous carbon, increases the contents of nitrogen element and sulfur element, enhances the interaction between the porous carbon and microorganisms, and significantly improves the removal rate of ammonia nitrogen.

The invention discloses a method for treating aquaculture wastewater by using compound bacteria. The method comprises the following steps: putting a compound bacteria agent into the aquaculture wastewater, and stirring; the preparation method of the complex microbial inoculant comprises the following steps: respectively inoculating enterococcus faecalis, pseudomonas pseudoalcaligenes and klebsiella into a liquid culture medium to obtain bacterial solutions of the enterococcus faecalis, the pseudomonas pseudoalcaligenes and the klebsiella; and adding porous carbon and bacterial liquid into the new liquid culture medium, and loading to obtain the complex microbial inoculant. During treatment, loss of enzymes and antibacterial peptides generated by microorganisms can be prevented, so that the removal rate of ammonia nitrogen in the aquaculture wastewater is increased, part of nickel is released in the degradation process, and the activity of the microorganisms is improved; the DL-methionine is mixed with the carbon precursor, so that the nickel species are well dispersed, the specific surface area of the porous carbon is also increased, the content of nitrogen and sulfur elements is increased, the interaction between the porous carbon and microorganisms is enhanced, and the removal rate of ammonia nitrogen is remarkably increased.

The invention provides a penicillium clavatum agent composition for improving saline-alkali soil and application of the penicillium clavatum agent composition, and belongs to the technical field of soil improvement. The composition is prepared from penicillium kirschwskii, pseudomonas alcaligenes and an artemisia sacrorum extract. Wherein the name of the penicillium kenii strain is penicillium kenii 23301-20, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41049, and the preservation unit is China General Microbiological Culture Collection Center. When the microbial agent composition is applied to saline-alkali soil, the ion content and the soil hardening condition can be effectively improved.

The invention discloses a preparation method of a bacterial strain for aerobic composting of kitchen waste and a grease degrading bacterial agent thereof, which is characterized by comprising the following steps of: enriching and domesticating a microbiota by using a hydrolysis ring method by adopting an enrichment domestication and separation purification method, separating and screening the bacterial strain for degrading animal grease, the method specifically comprises the following steps: collection of microbial communities, enrichment domestication, separation and purification of strains, and primary screening and secondary screening of the strains; the liquid grease degrading bacterial agent is prepared by compounding bacterial strain pseudomonas alcaligenes liquid, brevibacillus parabrevis liquid, candida lipolytica liquid and bacillus amyloliquefaciens liquid according to the volume ratio of 1: 1: 1: 1. The solid grease degrading microbial inoculum is prepared by mixing the liquid microbial inoculum and the conditioner according to the volume-weight ratio of the dry weight of the liquid microbial inoculum to the conditioner of 1: 1. Compared with the prior art, the method has the advantages that animal fat is efficiently degraded, the composting period is short, stink emission is avoided, the composting treatment effect is improved, efficient resourceful treatment of kitchen waste is promoted, the influence on the environment is reduced, and the method has good application prospects, economic value and social benefits.