Complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as preparation method and application thereof

A technology of composite bacterial agents and benzene series, which is applied in the fields of biotechnology and environmental protection, can solve the problems of limiting the scope of application of exogenous microbial bacterial agents, the use effect, and the slow degradation speed, so as to speed up the degradation speed, reduce the addition, cost-saving effect

Active Publication Date: 2020-08-14
BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, exogenous microorganisms and indigenous microorganisms have different characteristics. Most of the exogenous microorganisms are microorganisms with good petroleum hydrocarbon and benzene series degradation ability after screening, but the adaptability of exogenous microorganisms to the polluted soil environment and the indigenous The competitive relationship of microorganisms limits the application scope and application effect of exogenous microbial agents; the indigenous microorganisms are mostly the dominant flora in the polluted soil environment, and have good adaptability to the local environment and high degradation ability, but in the natural Limited by the physical and chemical properties of the polluted soil and the lack of nutrients such as nitrogen and phosphorus, the degradation rate is very slow

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  • Complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as preparation method and application thereof

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preparation example Construction

[0054] Four, the preparation of composite bacterial agent:

[0055] Mix the fermentation cultures of the components of the above obtained composite bacterial agent in proportion, and adjust the fermentation culture time and the mass percentage content of the solid fermentation culture to obtain a composite bacterial agent containing a specific effective number of viable bacteria.

[0056] In the composite microbial agent of the present invention, the mass percentage of the effective viable count of each strain accounting for the total effective viable bacterial count of the composite microbial agent is as follows:

[0057] Aspergillus oryzae 30-35%, Neurospora sp 30-35%, Pseudomonas putida 10-15%, Pseudomonas alcaligenes 10-15% %, Brevibacterium epidermidis 5-10%, Erwinia sp 5-10%.

[0058] The formula of above-mentioned each culture medium is as follows:

[0059] Strain preservation medium: beef extract 5g, peptone 10g, NaCl 5g, agar 20g, distilled water 1000mL, pH 7.0;

...

Embodiment 1

[0067] The present embodiment provides a kind of (stabilization of urban stock garbage) composite bacterial agent, the total effective number of viable bacteria of the composite bacterial agent is 10 10 cfu / mL, the effective viable count of each strain accounts for the mass percent of the total effective viable count of the composite microbial agent as follows:

[0068] Aspergillus oryzae 35%, Neurospora 30%, Pseudomonas putida 10%, Pseudomonas alcaligenes 10%, Brevibacterium epidermidis 10%, Erwinia 5%.

Embodiment 2

[0070] The present embodiment provides a kind of composite bacterial agent, the total effective number of viable bacteria of the composite bacterial agent is 10 10 cfu / mL, the effective viable count of each strain accounts for the mass percent of the total effective viable count of the composite microbial agent as follows:

[0071] Aspergillus oryzae 30%, Neurospora 30%, Pseudomonas putida 10%, Pseudomonas alcaligenes 15%, Brevibacterium epidermidis 5%, Erwinia 10%.

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Abstract

The invention relates to the field of biotechnology and environmental protection, in particular to a microbial inoculant, and particularly relates to a complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as a preparation method and application thereof. The active ingredients of the complex microbial inoculant comprise aspergillus oryzae, streptomyces, pseudomonas putida, pseudomonas alcaligenes, brevibacterium epidermidis and Erwinia; and the complex microbial inoculant is especially suitable for treating soil polluted by petroleum hydrocarbon and benzeneseries. After the complex microbial inoculant disclosed by the invention is added into the soil polluted by the petroleum hydrocarbon and benzene series, indigenous degrading flora can be rapidly propagated, and the remediation process can be rapidly started; in the repairing process, the indigenous degrading bacteria are always in the dominant flora status, and high degradation efficiency is kept for a long time through the synergistic cooperation of the flora in the complex microbial inoculant; and finally, the polluted soil is completely repaired, the degradation rate of petroleum hydrocarbon and benzene series is high, and the removal rate of BTEX and TPH can reach 98% or above.

Description

technical field [0001] The invention relates to the fields of biotechnology and environmental protection, in particular to a bacterial agent, in particular to a compound microbial agent for treating petroleum hydrocarbons and benzene series, as well as its preparation method and application. Background technique [0002] In the process of oil extraction, transportation, processing, and use, oil leakage will inevitably occur, causing environmental pollution. With the increasing demand for oil, the environmental pollution caused by it is becoming more and more serious; especially after the oil spill pollutes the soil, it destroys the ecological environment and production performance of the polluted land, causing a lot of waste of land and arable land resources; and , groundwater pollution due to infiltration, which in turn affects water security. In addition, the processing and production of petroleum products and the use of various products have also caused serious pollution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14B09C1/10C12R1/69C12R1/40C12R1/38C12R1/18C12R1/13C12R1/645
CPCC12N1/20C12N1/14B09C1/105B09C1/10B09C2101/00
Inventor 王迪宋磊李鹏飞李丽华吕秀芬陈蓓刘奇林王盟邓丽丽张梅宋坚崔宝山阚霏马卓然于诗卓叶齐荭
Owner BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA
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