75 results about "Brevibacterium sp." patented technology

The invention discloses a halophilic bacterial agent and a preparation method thereof as well as a biological treatment system fixed with the bacterial agent and application thereof. In the invention, strains are screened from sewage, sludge and soil to be prepared into a bacterial agent, wherein the bacterial agent comprises four strains including Providenciasp. CJ-4, Oceanobcillussp. CJ-10, Brevibacterium epidermidis CJ-12 and Halomonassp. CJ-13. The invention also provides the biological treatment system fixed with the bacterial agent and a method for treating waste water by using the same. The bacterial agent disclosed by the invention has stronger degradation capability on phenolic wastewater, can adapt to high-salt environments and has very good degradation effects under an environment with the salt content of 2-25% and the phenol concentration of 2500 mg/l; and compared with the traditional active sludge method, the bacterial agent has obvious advantages in the performance of degrading phenolic compounds, and the degradation effect on the phenolic compounds in water can reach more than 99.9%.

Two gene clusters have been isolated from an Brevibacterium sp HCU that encode the enzymes expected to convert cyclohexanol to adipic acid. Individual open reading frames (ORF's) on each gene cluster are useful for the production of intermediates in the adipic acid biosynthetic pathway or of related molecules. All the ORF's have been sequenced. Identification of gene function has been made on the basis of sequence comparison and biochemical analysis.

The invention discloses a brevibacterium halotolerans with relatively high azotase activity and application thereof in ecological restoration. The brevibacterium halotolerans GDSD21 is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.12402. Nitrogen-fixing bacteria are separated from rhizosphere soils of field-crop roots, further strains with relatively high azotase activity are screened, and finally the efficient nitrogen-fixing bacterium brevibacterium halotolerans GDSD21 is screened. The azotase activity of the brevibacterium halotolerans GDSD21 is obviously higher than that of common production strain azotobacter chroococcum ACCC11103 for microbial fertilizers, and the brevibacterium halotolerans GDSD21 has extensive application prospects in soil improvement, increasing of the soil fertility, ecological restoration of a virgin land, and production of industrialized seedling-rearing inoculation agents, nitrogen-fixing microbial agents and bio-organic fertilizers for vegetables.

The present invention belongs to the field of bioengineering technology and particularly relates to L-valine-producing brevibacterium flavum. The brevibacterium flavum is preserved in China Center forType Culture Collection on July 01, 2019, a preservation number is CCTCC M2019496 and a preservation address is Luojia Hill, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The present invention also relates to a method for producing L-valine by using the brevibacterium flavum, the brevibacterium flavum strain is subjected to activation culture and seed liquid culture, and obtained seedliquid is inoculated into a fermentation culture medium for fermentation culture, and the fermentation culture process is firstly aerobic fermentation and then anaerobic fermentation. L-valine production is carried out by using the preserved strain and the production method, yield is high, sugar-acid conversion rate is high, besides, fermentation process takes a short time, and production efficiency is greatly improved.

The invention discloses a process for producing arginine by microbial fermentation. Brevibacterium flavum MQA121 is adopted. The method comprises the following steps: preparing fermentation liquid, namely performing slant culture, performing shake-flask culture, performing primary seed culture, performing secondary seed culture, and continuing to ferment; extracting and refining a fermented product, namely preprocessing the fermentation liquid, performing secondary crystallization, performing color and impurity removal, performing membrane concentration and separation, drying, and packaging to obtain arginine as a finished product. The method has the benefits that organic and inorganic matters and bacteria in subsequent extracting and refining liquid of arginine can be remarkably reduced and the arginine yield can be increased; in addition, the product quality is improved and the production cost is reduced.

The invention relates to a construction method and applications of a genetic engineering bacterium for producing L-arginine. The invention discloses a novel genetic engineering bacterium strain for producing L-arginine. Recombinant plasmid pXMJ19-argH can be orderly converted and introduced into colibacillus and auxotroph brevibacterium flavum AN78 by the genetic engineering bacterium strain, and then the argininosuccinase in the AN78 containing recombinant plasmids is over-expressed and fermented to produce L-arginine. The invention further discloses a construction method and applications of the genetic engineering bacterium. The genetic engineering bacterium can increase the L-arginine production output and reduce the production cost.

The invention relates to a complex microbial inoculant. The complex microbial inoculant comprises eleven types of complex inoculants and accessories. The complex inoculants comprise seven types of bacteria such as saccharomyces cerevisiae, pseudomonas veronii and brevibacterium epidermidis and are added to the accessories, and the complex inoculants and the accessories are subjected to solid fermentation to obtain the complex microbial inoculant. The complex microbial inoculant has the advantages that kitchen waste can be effectively reduced by the complex microbial inoculant as compared with other commercially available inoculants, odor generated by the kitchen waste in compost maturity procedures can be effectively reduced, and the compost maturity level can be upgraded.

The invention relates to a 6-aminopenicillanic acid (6-APA) degrading bacterium and a screening method thereof. Particularly, the invention relates to a strain of 6-APA degrading bacterium Bordetella sp.L2, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 18, 2012, with the preservation number of CGMCC No. 6691; and the GenBank registration number of the 16S rRNA gene sequence of the 6-APA degrading bacterium Bordetella sp.L2 is HQ840720. The 6-APA degrading bacterium Bordetella sp.L2 is an aerobic bacillus brevis; and when the pH value is 8.0, the 6-APA degradation rate of the 6-APA degrading bacterium Bordetella sp.L2 is 28%. The strain achieves higher 6-APA degradation capability and has a practical significance and an important engineering application value for treatment of waste water containing 6-APA cephalosporin.

The invention discloses a bacterial strain Brevibacterium sp.YZ-1 used for arsenic polluted soil remediation and an application method of the bacterial strain used for arsenic polluted soil remediation Brevibacterium sp.YZ-1. The collection number of the strain is CGMCC No.8329. The toleration for As (III) reaches above 1000 mg/L, and an As (III) solution with the concentricity of 100 mg/L can be oxidized by over 75%. Through the strain, water-soluble As (III) of arsenic polluted soil can be reduced by 82.6%, and the effective state As (III) can be reduced by 84.1%. The arsenic virulence in the environment is greatly reduced. The research shows that the strain has good application prospects in the aspect of environment arsenic pollution control.

The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment.(A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or(B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

The invention belongs to the technical field of functional microbiological screening and application, and particularly relates to calcium carbonate producing actinomycetes and application thereof. The calcium carbonate producing actinomycetes is brevibacterium linens BS258, a strain is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms in Number 3 (the Institute of Microbiology) of the Number 1 courtyard on the West Beichen Road in the Chaoyang District of Beijing on September 26th, 2016, a preservation number of the strain is CGMCC 1.15904, and a taxonomy name of the strain is brevibacterium linens BS258. The calcium carbonate producing actinomycetes and the application have the advantages that calcium carbonate precipitates can be induced by the actinomycetes, and the strain can tolerate high-calcium-ion environments and can resist diversified heavy metal ions; calcium ions in the environments can be induced under the effect of carbonic anhydrase by the aid of alkaline micro-environments generated in urea decomposition procedures to form the calcium carbonate precipitates, the heavy metal ions can be co-precipitated in calcium carbonate forming procedures, accordingly, the purposes of biological mineralization and heavy metal restoration can be achieved, and the shortcomings in the prior art can be overcome.

The invention relates to chlorine proof microorganism. It is chlorine proof strain No.1. Its features are as follows: it is brevibacterium linens B723-1 CCTCC NO.M205122; its colonial morphologies are creamy white, rule circular, un-transparent, smooth, stiff, and pink after 5M KOH processing; physiological characteristics that young bacterial is irregular baculiform, 0.6-1.2um*1.5-6.0um, single or pairs arrangement, common V shape; old is irregular globular, and gram positive; its main biological and chemical features are concurrently character anaerobic, chemoheterotrophic bacteria, contacting enzyme positive, oxidase negative, producing arginine double hydrolase, lysine decarboxylase, ornithine decarboxylase, and urease, and producing acid from glucose, no moving, no spore; its optimum temperature is 25-37 centigrade degree. It can live in high concentration chloride ion condition, and high effectively degrade high concentration organic pollutant.

The invention belongs to the technical field of microbial preparations, and relates to a microbial preparation for promoting plant root system growth and a preparation method thereof. The microbial preparation comprises 60-70 parts of starch, 8-10 parts of a special microbial agent, and 20-32 parts of algal polysaccharide, wherein the microbial agent is low-altitude and normal-temperature domesticated Brevibacterium sp. TS22, and a preservation number is CGMCC 16576; and the microbial agent further comprises a mixed bactericide of a phosphate solubilizing bacterial strain and a soil rooting agrobacterium, and a weight is 0-20% of the microbial agent. The preparation method for the microbial preparation comprises the following steps: putting raw material components of the microbial preparation in parts by weight into a stirrer, stirring and reacting, to obtain the microbial preparation for promoting the plant root system growth. The microbial preparation is independently used or compounded with an agriculture and forestry organic fertilizer for using. The microbial preparation is simple and convenient in operation, low in production cost, and capable of remarkably improving the rootsystem growth of a plant.

The invention discloses an application of ZM brevibacterium to make biological umangite with reserving name as Sporocytophaga sp. and reserving data in the Apr. 21th 2006 and reserving number at CGMCC1694, which comprises the following steps: (A) inoculating ZM bacterium in the culture medium; (B) 1) adding bacterium liquid and sodium selenite in the culture medium; displaying the ferment liquid from light yellow into red to slide through microscope under visible red light; 2) condensing to obtain the product.

The invention discloses brevibacterium and a hydrolytic synthesis method of alpha-cyclo hexyl mandelic acid through nitrile and a derivative, which belongs to microbe technical field. A preservation number of brevibacterium sp.CCZU12-1 is CGMCCNo. 7042, and the brevibacterium is suggested to be classified and named brevibacterium sp.. alpha-cyclohexyl mandelonitrile and its derivative are taken as an initial raw material, a brevibacterium sp.CCZU12-1 resting cell is taken as a biocatalyst, and then the biosynthesis of alpha-cyclo hexyl mandelic acid and its derivative can be carried out under certain condition. The method provided by the invention has the characteristics of simple technical route, mild reaction condition and no pollution.

The invention discloses an indium oxide nanosphere coating brevibacterium flavum nitrile hydratase, a preparation method and application. The multilayered structure of cells is simulated by referring to the existence form of the hydratase in a living body, and the indium oxide nanosphere is prepared through bionic design of components, functions and processes and used for embedding of the brevibacterium flavum nitrile hydratase. The characteristics that the immobilized nitrile hydratase is high in stability, high in activity, easy to recycle and the like are used for catalyzing 3-cyanopyridine to be transformed into nicotinamide in an efficient hydrolysis mode. The preparation method of the bionic immobilized hydratase can provide reference significance for preparation of a microcyst immobilized carrier, and provide a novel research idea for biotransformation of the 3-cyanopyridine.

The invention aims at providing a fermentation process for increasing the yield of L-arginine. The adopted technical scheme is as follows: brevibacterium flavum ATCC21493 (SD<R>) is adopted as a starting bacterium, a strain TA189 is obtained through NTG (Nitrosoguanidine) treatment and mutagenesis and can directly synthesize arginine by using glucose, the arginine production capacity is strong, the strain production capacity and the fermentation process are controlled to reach indexes, and the comprehensive level of the process reaches the leading domestic level. According to the fermentation process provided by the invention, the characteristics of the fermentation processes of amino acid and antibiotics are combined, the vitality of starting and ending thalli is strictly controlled by adopting trend control and a midway feed and glucose supplementing process, and a relatively high arginine production level is obtained.

The invention discloses a complex microbial inoculant for efficiently degrading kitchen waste and a preparation method thereof, and is characterized in that the complex microbial inoculant is prepared from 20-30 parts of bacillus licheniformis suspension, 10-20 parts of bacillus amyloliquefaciens suspension, 10-20 parts of bacillus subtilis suspension, 10-15 parts of bacillus licheniformis suspension, 5-15 parts of brevibacterium suspension and 5-10 parts of aspergillus versicolor suspension. The preparation method comprises the following steps: activating 10-25 parts of paenibacillus polymyxa bacterial suspension, 5-10 parts of beer yeast wine variant bacterial suspension, 5-10 parts of streptomyces thermophilus bacterial suspension and 5-15 parts of lysinibacillus xylolyticus bacterial suspension, carrying out expanded liquid culture, preparing concentrated bacterial suspension by a centrifugation method, adding the bacterial suspension of each strain according to a certain ratio, carrying out fermentation, and carrying out freeze-drying to obtain the microbial inoculant containing the paenibacillus polymyxa, the beer yeast wine variant bacterial suspension, the streptomyces thermophilus and the lysinibacillus xylolyticus. A high-efficiency bacterial strain composite system is constructed, and the high-efficiency bacterial strain composite system is uniformly mixed with a carrier material composed of sawdust, bran and activated carbon in a certain mass ratio, so that the composite microbial preparation is prepared, and high-efficiency degradation of the kitchen waste is realized.

The present invention belongs to the technical field of micro-organism preparations and relates to a micro-organism preparation for enhancing lawn growth ability and a preparation method thereof. Themicro-organism preparation comprises 60-70 parts of starch, 8-10 parts of a micro-organism agent and 20-32 parts of seaweed polysaccharides. The micro-organism agent is brevibacterium sp. TS22 acclimated at low altitude and normal temperature and a preservation number is CGMCC 16576. The micro-organism agent can also comprise gibberellin-producing bacteria and weight of the gibberellin-producing bacteria is 0-20% of the micro-organism agent. The preparation steps of the micro-organism preparation are as follows: raw material components of the micro-organism preparation are put into a stirrer according to parts by weight and a stirring reaction is conducted to obtain the micro-organism preparation for enhancing the lawn growth ability. The micro-organism preparation can be used alone or incombination with compound agricultural and forestry organic fertilizers. The micro-organism preparation is simple to operate and low in production costs, and can effectively promote the lawn growth.