Chlorine resisting strain No.1 and screening process thereof

A screening method and strain technology are applied in the field of chlorine-resistant microorganisms and their screening to achieve the effect of good chlorine-resistant ability and wide range

Inactive Publication Date: 2006-06-28
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] However, not all chlorine-resistant microorganisms can be applied to the treatment of wastewater containing high concentrations of chloride ions. Strict screening and domestication are required to obtain special resistant microorganisms that can not only tolerate high concentrations of chloride ions, but also degrade high-concentration organic matter in wastewater. Chlorine microorganisms

Method used

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  • Chlorine resisting strain No.1 and screening process thereof
  • Chlorine resisting strain No.1 and screening process thereof
  • Chlorine resisting strain No.1 and screening process thereof

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Experimental program
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Effect test

Embodiment Construction

[0017] 1. Screening method for a chlorine-resistant bacterial strain No. 1:

[0018] (1) Material preparation:

[0019] 1. The experimental wastewater and bottom sludge were taken from the saponin wastewater regulating tank of Fangkun Real Estate Co., Ltd., Shiyan, Hubei Province.

[0020] 2. Medium: SM medium: peptone 10g; beef extract 5g; distilled water 1000ml; agar 20g (solid); CaCl 2 15.6g / L, pH=7.5.

[0021] SC medium: In SM medium, add different concentrations (90,000, 80,000, 70,000, 60,000, 50,000, 40,000, 30,000, 20,000, 10,000, 5,000 mg / L) according to the experimental needs of chloride ions.

[0022] 3. Experimental instruments and equipment:

[0023] Guohua SHA-B constant temperature oscillator,

[0024] SHH150G photobiological incubator,

[0025] Horizontal pressure steam sterilizer-----Shanghai Medical Nuclear Instrument Factory,

[0026] WMX-1 microwave sealed digestion COD speed tester / -----Shantou Huanhai Engineering Corporation,

[0027] 722S Spectro...

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Abstract

The invention relates to chlorine proof microorganism. It is chlorine proof strain No.1. Its features are as follows: it is brevibacterium linens B723-1 CCTCC NO.M205122; its colonial morphologies are creamy white, rule circular, un-transparent, smooth, stiff, and pink after 5M KOH processing; physiological characteristics that young bacterial is irregular baculiform, 0.6-1.2um*1.5-6.0um, single or pairs arrangement, common V shape; old is irregular globular, and gram positive; its main biological and chemical features are concurrently character anaerobic, chemoheterotrophic bacteria, contacting enzyme positive, oxidase negative, producing arginine double hydrolase, lysine decarboxylase, ornithine decarboxylase, and urease, and producing acid from glucose, no moving, no spore; its optimum temperature is 25-37 centigrade degree. It can live in high concentration chloride ion condition, and high effectively degrade high concentration organic pollutant.

Description

technical field [0001] The invention relates to a chlorine-resistant microorganism and a screening method thereof. Background technique [0002] Ordinary microorganisms have limited tolerance to chloride ions. Generally, when the concentration of chloride ions is less than 4000mg / L, microorganisms can grow without significant inhibition. However, if the concentration of chloride ions is greater than 4000mg / L, the inhibitory or even toxic effect of chloride ions on microorganisms will appear sharply with the increase of chloride ion concentration, resulting in the inability of ordinary microorganisms to survive. However, in nature, there are also some special microorganisms that can grow normally in environments containing high concentrations of chloride ions, even extremely high concentrations of chloride ions (5000-80000 mg / L). These microorganisms can be called chlorine-resistant microorganism. [0003] However, not all chlorine-resistant microorganisms can be applied to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/10C12N15/31C12R1/13
Inventor 鲍建国王焰新刘慧张彩香信欣李耀辰刘双李平张莉君
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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