Preparation method of bionic immobilized 3-cyanopyridine nitrile hydratase
A technology of cyanopyridine nitrile hydration and nitrile hydratase, which is applied in the field of microorganisms, can solve the problems of many reaction steps, cumbersome preparation of enzyme-producing cells, and large amount of catalyst used in chemical methods, so as to achieve high recovery rate and improve the stability of repeated use Effect
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[0026] A preparation method of biomimetic immobilized nitrile hydratase adopts biomimetic immobilized enzyme, the enzyme is formed by Brevibacterium flavum nitrile hydratase embedded in indium oxide nano shell, the specific preparation method comprises the following steps:
[0027] (1) Preparation of liposome template: Add 15 g of soybean lecithin and 5.5 g of cholesterol into 85 mL of ethanol solution, stir evenly; remove ethanol by rotary evaporation at 40°C until a uniform and transparent film is formed on the reactor; then add 10 mL of 0.5 g / L Brevibacterium flavum nitrile hydratase solution (prepared with 0.1 mol / L, pH=7 disodium hydrogen phosphate-citrate buffer) was used to dissolve the film, and the final concentration was 20 g / L liposome solution;
[0028] (2) Preparation of liposome microcapsules: take the above liposome solution, and slowly drop 1 mL of the inducer trimellitic acid (the concentration of trimellitic acid is 1.5 g / L) under the condition of vigorous s...
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