L-valine-producing brevibacterium flavum and method for producing L-valine by using brevibacterium flavum

A technology of Brevibacterium flavum and valine, applied in the field of bioengineering, can solve the problems of low sugar-acid conversion rate of glucose yield, long time consumption, and low efficiency, so as to increase sugar-acid conversion rate, reduce glucose, and improve production efficiency Effect

Active Publication Date: 2020-01-03
BAYANNUR HUAHENG BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation and cultivation process of this method needs to maintain a specific oxygen pressure, and the cultivation process takes a long time, about 90 hours, and the glucose yield (ie sugar-acid conversion rate) is low, only 25-30%.
Visible, adopt this method to produce L-valine, efficiency is relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The above-mentioned Brevibacterium flavum (Brevibacterium flavum) HHVLA-002 was inoculated in the activated slant medium and cultured to the logarithmic phase. The activated slant medium: peptone 6.0g / L, sodium chloride 3.0g / L, yeast powder 3.0g / L , beef extract 3.0g / L, agar 15g / L, adjust the pH value to 7.0-7.2; cultivate at 30±1℃ for 20h.

[0035] Inoculate the bacterium colony obtained after activation in the shake flask seed medium for seed culture, seed medium: glucose 10g / L, diammonium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 5g / L, magnesium sulfate heptahydrate 0.2g / L L, ferrous sulfate heptahydrate 0.01g / L, vitamin B1 50μg / L, adjust the pH value to 6.5-7.8 with ammonia water; cultivate at 28±1°C and 180r / min for 24h to obtain seed liquid.

[0036] Get the seed liquid obtained above, carry out fermentation culture under the following fermentation medium and culture conditions, produce L-valine, fermentation medium: glucose 160g / L, diammonium hydrog...

Embodiment 2

[0040] The above-mentioned Brevibacterium flavum (Brevibacterium flavum) HHVLA-002 was inoculated in the activated slant medium and cultured to the logarithmic phase, the activated slant medium: peptone 7.0g / L, sodium chloride 4.0g / L, yeast powder 4.0g / L , beef extract 4.0g / L, agar 15g / L, adjust the pH value to 7.0-7.2. Incubate at 30±1°C for 20h.

[0041] Inoculate the bacterium colony obtained after activation into the shake flask seed medium for seed culture, seed medium: glucose 13g / L, diammonium hydrogen phosphate 6g / L, potassium dihydrogen phosphate 6g / L, magnesium sulfate heptahydrate 0.3g / L L, ferrous sulfate heptahydrate 0.01g / L, vitamin B1 80μg / L, adjust the pH value to 6.5-7.2 with ammonia water; culture at 28±1°C and 180r / min for 24h to obtain seed liquid.

[0042] Get above-mentioned gained seed liquid, carry out fermentation culture under following fermentation medium and culture condition, produce L-valine:

[0043] Fermentation medium: glucose 180g / L, diammon...

Embodiment 3

[0047] The above-mentioned Brevibacterium flavum (Brevibacterium flavum) HHVLA-002 was inoculated in the activated slant medium and cultured to the logarithmic phase. The activated slant medium: peptone 6.0g / L, sodium chloride 7.0g / L, yeast extract 10.0g / L , beef extract 4.0g / L, agar 15g / L, adjust the pH value to 7.0-7.2. Incubate at 30±1°C for 20h.

[0048] Inoculate the bacterium colony obtained after activation into the shake flask seed medium for seed culture, seed medium: glucose 20g / L, diammonium hydrogen phosphate 15g / L, potassium dihydrogen phosphate 6g / L, magnesium sulfate heptahydrate 0.3g / L L, ferrous sulfate heptahydrate 0.01g / L, vitamin B1 120μg / L, adjust the pH value to 6.5-7.2 with ammonia water; culture at 28±1°C and 180r / min for 24h to obtain seed liquid.

[0049] Get above-mentioned gained seed liquid, carry out fermentation culture under following fermentation medium and culture condition, produce L-valine:

[0050] Fermentation medium: glucose 200g / L, dia...

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Abstract

The present invention belongs to the field of bioengineering technology and particularly relates to L-valine-producing brevibacterium flavum. The brevibacterium flavum is preserved in China Center forType Culture Collection on July 01, 2019, a preservation number is CCTCC M2019496 and a preservation address is Luojia Hill, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The present invention also relates to a method for producing L-valine by using the brevibacterium flavum, the brevibacterium flavum strain is subjected to activation culture and seed liquid culture, and obtained seedliquid is inoculated into a fermentation culture medium for fermentation culture, and the fermentation culture process is firstly aerobic fermentation and then anaerobic fermentation. L-valine production is carried out by using the preserved strain and the production method, yield is high, sugar-acid conversion rate is high, besides, fermentation process takes a short time, and production efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an L-valine-producing Brevibacterium flavum and a method for producing L-valine by using the bacterium. Background technique [0002] L-valine, also known as 2-amino-3-methylbutyric acid, belongs to branched-chain amino acid, has a variety of physiological functions, and has significant functions in the metabolic process of human body and other animals. It can be used in pharmaceutical industry, food industry and feed industry, etc. [0003] In the field of medicine, L-valine is the raw material of compound amino acid infusion and amino acid injection. It is widely used in dietary therapy, treatment of sepsis and postoperative diabetic patients, treatment of surgical wound healing and nutritional support treatment of tumor patients. [0004] With the rapid development of L-valine from traditional feed additives to high value-added industries such as food, med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12P13/08C12R1/13
CPCC12N1/36C12N1/20C12P13/08C12R2001/13C12N1/205
Inventor 韩成秀张学礼杨林郭恒华张冬竹刘树蓬
Owner BAYANNUR HUAHENG BIOTECHNOLOGY CO LTD
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