108 results about "Sugar acids" patented technology

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention discloses a spermidine synthetase mutant and application of the spermidine synthetase mutant in synthesis of spermidine, and belongs to the technical field of genetic engineering and metabolic engineering. According to the method, the spermidine synthetase is mutated firstly, the efficiency of the spermidine synthetase is improved, and on the basis, the dissolved oxygen and fed-batch concentration are optimally controlled through fermentation, so that the yield of spermidine is improved. The corynebacterium glutamicum provided by the invention is an engineering bacterium with plasmids and without defects, and after the corynebacterium glutamicum is subjected to fed-batch fermentation in a 5L fermentation tank for 68 hours, the yield of spermidine reaches 25.1 g / L, and the sugar-acid conversion rate is 17.9%.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol through two-step enzyme catalysis of hemicellulose hydrolysate, and the method comprises the following steps: taking hemicellulose hydrolysate as a raw material, adding arabinose dehydrogenase, NADH oxidase and coenzyme NADH, and carrying out first-step fermentation until no L-arabinose residue exists in fermentation liquor to obtain fermentation reaction liquor I; and adding xylose reductase and glucose dehydrogenase into the fermentation reaction liquid I, and carrying out second-step fermentation to obtain a fermentation product containing xylitol. According to the two-step enzyme catalysis method provided by the invention, xylitol is prepared, and high-purity xylose does not need to be used as a raw material; according to the present invention, the cheap hemicellulose hydrolysate can be directly adopted as the raw material, the xylose conversion rate and the xylitol yield can exceed 99%, the impurity arabitol content in the product is low, the other impurity saccharic acid is easy to separate, and the problems of complex process, large separation difficulty and high cost in the xylitol refining process are successfully solved.

The invention discloses a method for improving the synthesis level of L-tryptophan in escherichia coli based on ARTP mutagenesis high-throughput screening, and belongs to the technical field of bioengineering. According to the invention, the L-tryptophan biosensor pSensor-trp3 is constructed, and the L-tryptophan biosensor pSensor-trp3 is good in response under the condition that the exogenous addition concentration range of L-tryptophan is 0-300mg. L <-1 >. High-throughput screening is performed by using a flow cytometry, a dominant mutant strain YB-2 is obtained through iterative mutagenesis, and the shake flask L-tryptophan yield and the sugar-acid conversion rate of the dominant mutant strain YB-2 are 5.68 g.L <-1 > and 0.103 g.g <-1 > respectively and are increased by 11.4% and 10.7%. Through second-generation whole genome re-sequencing comparative analysis, it is speculated that the genes ynfB and waaO possibly have a promoting effect on synthesis of L-tryptophan. The L-tryptophan biosensor used in the invention also provides reference for high-throughput screening and transformation of other related L-tryptophan production strains.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention provides a high-yield O-succinyl-L-homoserine engineering bacterium as well as a construction method and application of the high-yield O-succinyl-L-homoserine engineering bacterium. According to the invention, O-succinyl-L-homoserine genetically engineered bacteria with high yield of O-succinyl-L-homoserine are constructed by introducing O-succinyl-homoserine transferase from Vibrio cholera into a chassis strain in an exogenous manner and obtaining a gene mutant metAVc (T242A) of the O-succinyl-L-homoserine transferase, and further increasing the expression of the O-succinyl-L-homoserine transferase by adopting a Trc promoter. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 18.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 105.4 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention belongs to the technical field of microorganisms, and particularly relates to a strain for producing high-optical-purity L-lactic acid and application of the strain. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 13, 2025, and the preservation number of the strain is CGMCC No. 34537; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. When the strain is used for fermentation production of L-lactic acid, the fermentation period is short, the sugar-acid conversion rate is high, the yield of L-lactic acid is high, and the optical purity is high. In addition, the strain also has excellent growth and metabolism stability, lactic acid with optical purity can still be efficiently produced after passage to the tenth generation, and the strain is suitable for industrial production of high-purity L-lactic acid.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The invention provides a method for improving fermentation yield and sugar-acid conversion rate of 5-hydroxytryptophan, alpha-ketoglutaric acid ferrous salt, alpha-ketoglutaric acid zinc salt, alpha-ketoglutaric acid cobalt salt and alpha-ketoglutaric acid manganese salt are added into a fermentation culture medium according to a specific proportion, alpha-ketoglutaric acid directly participates in TCA circulation, circulation operation can be accelerated, generation of NADH and FADH2 is increased, the yield of 5-hydroxytryptophan is increased, and the yield of 5-hydroxytryptophan is increased. The ATP synthesis efficiency is improved, and more energy is provided for cell growth; the alpha-ketoglutaric acid can balance amino acids required in the growth process of escherichia coli, promote the generation of proteins and nucleic acids and accelerate the growth of thalli; the alpha-ketoglutaric acid metal salt can improve the utilization efficiency of microelements by thalli; ferrous ions are cofactors of 5-hydroxytryptophan hydroxylase and can accelerate the hydroxylation process of tryptophan. Through the synergistic effect of the added components, the yield of the 5-hydroxytryptophan and the sugar-acid conversion rate are improved.

The invention discloses a nutrition regulating agent for regulating the mature period of citrus fruits and application of the nutrition regulating agent. The nutrition regulating agent is prepared from potassium nitrate, monopotassium phosphate, magnesium sulfate, ferric sodium ethylenediaminetetraacetate, zinc sodium ethylenediaminetetraacetate, boric acid, ammonium molybdate, gibberellin, abscisic acid and diatomite which is supplemented to 100 parts. The invention further provides a preparation method of the nutrition regulating agent and application of the nutrition regulating agent in regulating the mature period of citrus fruits, and the application mode is that when the diameter of the citrus fruits is 40-50 mm, the preparation is diluted by 600-800 times to be sprayed on leaf surfaces, once every 7 days and twice continuously. Through the synergistic effect of mineral nutrient elements and low-concentration hormones, bidirectional precise regulation and control of the citrus mature period are achieved, fruits can be promoted to mature in advance or later for 7-15 days, the sugar-acid ratio and color of the fruits can be remarkably improved, meanwhile, the problems of tree vigor decline and quality decline caused by single hormone treatment are solved, and the method is suitable for large-scale popularization and application. The method has the characteristics of high efficiency, safety and simple operation.

The application provides a compound microbial fertilizer, a preparation method and application thereof. The compound microbial fertilizer comprises the following components in parts by weight: 10-30 parts of a compound microbial agent, 20-50 parts of an agent carrier, 30-50 parts of a compound fertilizer slow-release agent and 10-30 parts of modified urea aldehyde. The compound microbial fertilizer prepared by the application can be applied to growth tests of tomatoes, and higher yield, stem thickness growth value, vitamin C content and sugar acid ratio can be obtained. The compound microbial fertilizer has long-term effect, can release phosphorus, nitrogen and potassium and the like, has the effects of moisturizing and loosening soil, can increase the yield and quality of tomato fruits, promote the robustness of roots and stems and improve the eating taste.

Provided is a composition for inhibiting the proliferation of bacteria belonging to the genus Collinsella, the composition containing at least one selected from sugar acid-containing oligosaccharides and salts thereof. The sugar acid is preferably selected from aldonic acid, uronic acid, and aldaric acid.

According to the method for improving the flavor of the Huangguo pear juice beverage, provided by the invention, the Huangguo pear residues can be effectively utilized to extract aroma components, and the original fruit flavor of the Huangguo pear juice beverage is greatly improved through scientific process steps such as composite enzymolysis, reasonable adjustment of the sugar-acid ratio and strict sterilization treatment; the loss of aroma substances is reduced, so that the product has higher typicality and high quality, and a new technical support and a new development direction are brought to the processing industry of the Huangguo pears in the same kernel region.

Aiming at a lactic acid return loss phenomenon existing in a process for producing lactic acid by microbial fermentation, the invention firstly provides application of a target gene in inhibiting lactic acid return loss in lactic acid production by a microbial fermentation method, and the target gene is a lactic acid permease gene lutP and / or an iron-sulfur oxidase gene cluster lutABC. On the basis of the application, the invention also provides a method for inhibiting lactic acid return consumption in lactic acid production by a microbial fermentation method, and a bacillus coagulans engineering strain with low lactic acid return consumption and high sugar-acid conversion rate. According to the method, effective inhibition of intracellular transport and / or metabolism of lactic acid is realized, so that the problem of lactic acid return consumption in a process for producing lactic acid through microbial fermentation is solved, and the technical effect is remarkable. According to the engineering strain, a LutR palindromic sequence in a lactic acid permease gene lutP promoter region is modified, and / or an iron-sulfur oxidase gene cluster lutABC is inactivated, so that transmembrane transport and / or intracellular metabolism of lactic acid are / is effectively inhibited, a lactic acid return loss phenomenon is remarkably weakened, the sugar-acid conversion efficiency is improved, the fermentation process flow is simplified, the production cost is reduced, and the engineering strain is suitable for industrial production. And the method has important value for large-scale production.

The invention relates to flavored fruit vinegar and a preparation method thereof, and the flavored fruit vinegar has the beneficial effects that the coexistence of flavor and nutrition is realized through the matching of apples and roxburgh roses; wherein the apple juice is used as a fermentation substrate, so that stable sugar-acid ratio and mellow feeling are provided; the rosa roxburghii tratt greatly enriches active ingredients such as vitamin C, SOD, polyphenol and the like, and lays a health-care foundation of the product. In the fermentation process, the synergistic effect of saccharomycetes, acetic bacteria and lactic acid bacteria is adopted, the saccharomycetes efficiently complete alcoholic fermentation, the acetic bacteria dominate acid production, the lactic acid bacteria not only can generate soft lactic acid, more importantly, metabolites of the lactic acid bacteria can effectively complex and convert tannin astringency in the roxburgh roses, and the taste is fundamentally improved. The final product successfully integrates the mellowness of the apples and the high nutrition of the roxburgh roses and is free of obvious bitter and astringent taste, and the taste, flavor and nutritional value are comprehensively improved.

The present invention provides a tegoprazan sugar acid salt and a preparation method therefor, the salt being pharmaceutically usable for gastroesophageal disease, gastroesophageal reflux disease, gastritis, peptic ulcer, gastric ulcer, duodenal ulcer, NSAID-induced ulcer, non-erosive reflux disease (NERD) and the like. The tegoprazan sugar acid salt of the present invention has excellent water solubility and stability of tegoprazan, and thus can be particularly useful as a pharmaceutical injection preparation.

The invention relates to a monophase gel consisting of a non-polar liquid mixed with a non-ionic surfactant selected from ester surfactants of formula ROC(O)R1 or RC(O)OR2 or ether surfactants of formula ROR2, wherein RO denotes the radical of a sugar or sugar alcohol, RC(O)O denotes a sugar acid, C(O)R1 denotes the radical of a fatty acid and R2 denotes the radical of a fatty acid alcohol. In the afore-mentioned non-ionic surfactants, the sugar is a C5 or C6 sugar, and the sugar acid and the sugar alcohol are a sugar acid derived therefrom or a sugar alcohol derived therefrom. The fatty acid or fatty acid alcohol has a C chain length of at least 6 and the non-ionic surfactant has an OH group to C atom ratio in the range of 1: 3 to 1: 8. The invention further relates to methods for producing corresponding monophase gels and to the use of corresponding gels for texturing food or cosmetic products or for formulating pharmaceuticals.

The application discloses a kind of high-yield O-succinyl-L-homoserine recombinant escherichia coli and its construction method and application, belong to genetic engineering and fermentation engineering technical field.The recombinant escherichia coli is expressed by expressing anti-feedback inhibition homoserine O-succinyltransferase metA fbr , aspartate ammonia-lyase aspA, aspartate transaminase aspC, 2 ketoglutarate decarboxylase sucA, dihydrothioctic acid succinyltransferase sucB and efflux protein yjeH, knock out metJ, improve intracellular NADPH availability, to enhance OSH synthesis pathway metabolic flow, precursor succinyl coenzyme A supply and product efflux.Using 5 L bioreactor for fed-batch fermentation, after fermentation 60 h, OSH production is as high as 137.4 g / L, and sugar acid conversion rate reaches 50.5%.The application provides engineering strain and technical realization path for the efficient fermentation of OSH.

Various processes for preparing C4 aldoses and / or ketones thereof are described. Various processes are described for preparing C4 aldoses and / or ketones thereof from feed compositions comprising glycolaldehyde. Also, various processes for preparing useful downstream products and intermediates, such as erythritol and erythronic acid, from the C4 aldoses and / or ketones thereof are described.

The application discloses a kind of recombinant escherichia coli for producing L-tyrosine and application, belong to genetic engineering and bioengineering technical field.The application knocks out aroP and tyrP gene, expresses the yddG gene of escherichia coli endogenous to modify the aromatic amino acid transport system of escherichia coli, then heterologous expression comes from bifidobacterium fpk and expresses the ppsA and tktA gene of escherichia coli endogenous to increase the precursor supply of shikimic acid pathway, again through the anti feedback gene expression aroG and tyrA 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase, branch acid mutase and prephenate dehydrogenase are removed feedback inhibition, increase the carbon flux of shikimic acid pathway.Knock out tyrR gene removes the repression of TyrR protein to shikimic acid pathway key enzyme, again knock out trpE and pheA gene to block partial side reaction of shikimic acid pathway, so that the synthesis flux of L-tyrosine increases.Finally knock out endogenous poxB gene to realize the modification of acetic acid pathway, realizes the stable fermentation performance of high glucose concentration.

Methods of obtaining aggregates and / or pulverulent mineral material from a starting material comprising hardened mineral binder and aggregates utilizing process auxiliaries selected from the group consisting of polycarboxylate ethers and / or esters (PCE), glycols, organic amines, especially alkanolamines, ammonium salts of organic amines with carboxylic acids, surfactants, especially nonionic surfactants, gemini surfactants, calcium stearate, alkoxylated phosphonic or phosphoric esters, propane-1,3-diol, carboxylic acids, sulfonated amino alcohols, boric acid, salts of boric acid, borax, salts of phosphoric acid, gluconate, iron sulfate, tin sulfate, antimony salts, alkali metal salts, alkaline earth metal salts, lignosulfonates, glycerol, melamine, melamine sulfonates, water absorbents in the form of a superabsorbent polymer or in the form of a sheet silicate, anticaking agents, sugars, sugar acids, sugar alcohols, phosphates, phosphonates, and mixtures thereof.

The present application relates to the technical field of genetic engineering, and particularly relates to a recombinant microorganism, a preparation method thereof and application of the recombinant microorganism in threonine production. Expression of tpx gene and / or yodD gene in the recombinant microorganism is inhibited. The present application finds that inhibition of tpx gene or yodD gene in a strain can effectively improve the ability of the strain to produce amino acids, and the threonine production capacity of Escherichia coli with tpx gene and yodD gene knocked out can reach 27.35 g / L, and the sugar acid conversion rate can reach 27.94%, which has important significance in the field of threonine production.

A non-coding sRNA in Corynebacterium glutamicum and the use thereof. The non-coding sRNA has an RNA sequence having at least 90% or more sequence homology to a transcription product of the DNA sequence as shown in SEQ ID NO: 1. It is verified by means of experiments that the over-expression of the non-coding sRNA in a recombinant bacterium can significantly improve the yield of branched-chain amino acids, such as L-leucine, L-isoleucine and L-histidine, and the sugar-acid conversion rate, and also reduce the generation of by-product acid impurities. In terms of materials, relatively inexpensive molasses is selected, which provides rich carbon and nitrogen sources, to replace corn syrup which has more impurities. Moreover, the molasses is converted by means of enzymolysis into a nutrient substance containing more carbon and nitrogen sources that can be utilized by the strain. In addition, in order to control the carbon-to-nitrogen source ratio in a fermentation broth, a more accurate feeding process is selected, which can not only ensure the fermentation requirements of the strain, but can also effectively prevent excessively high or low nitrogen source levels in the fermentation broth, thereby achieving the aims of increasing the yield and the sugar-acid conversion rate, and reducing the by-product acid impurities.

The application discloses a method for producing lysine by immobilized continuous fermentation, and the method comprises the following steps: soaking a multi-hydroxy flexible polyester fiber carrier in soluble pectin, and drying the carrier by a dry heat method to obtain an immobilized carrier; inoculating seed liquid of cultured Corynebacterium glutamicum into a high-oxygen-bio-reactor containing the immobilized carrier and a fermentation medium, and performing batch-fed fermentation for a certain batch to immobilize the Corynebacterium glutamicum on the immobilized carrier, so that the Corynebacterium glutamicum can stably produce lysine; and switching to a mode for continuously producing lysine. The method for producing lysine by the immobilized Corynebacterium glutamicum fermentation can be stably operated for more than 15 days, and compared with free fermentation, the lysine production rate is increased by nearly 35%, and the sugar-acid conversion rate is increased by nearly 4 percentage points.

The application belongs to the technical field of biology, and discloses a strain for efficiently fixing carbon dioxide and improving succinic acid conversion rate and a construction method thereof. The application discloses a genetically engineered bacterium, which is obtained by overexpressing carbonic anhydrase genes, phosphoenolpyruvate carboxylase genes and phosphoenolpyruvate carboxykinase genes in a succinic acid-producing strain. ‑ The carbonic anhydrase can enhance the conversion efficiency of CO2 to HCO3 , and the phosphoenolpyruvate carboxylase and the phosphoenolpyruvate carboxykinase can realize efficient fixation of C3 and CO2 into C4 intermediates. Experiments prove that the genetically engineered bacterium can effectively produce succinic acid by using glucose, and has a relatively high sugar acid conversion rate (up to 79.80%).

The invention provides a method for improving the sugar-acid conversion rate in a shikimic acid fermentation production process. Comprising the following steps: S1, respectively preparing a seed culture medium and a fermentation culture medium, wherein the fermentation culture medium contains 0.1-0.4 g / L of methyl-alpha-D-glucopyranoside; s2, a seed culture stage: sequentially inoculating the escherichia coli bottle-combining seed solution into a primary seed tank and a secondary seed tank, and respectively culturing for a period of time; s3, fermentation culture stage: supplementing the culture solution after seed culture into a three-stage fermentation tank, and controlling the pH value of the fermentation solution to be 6.7-6.9 within 0-12 hours; the pH value of the fermentation liquor is 6.4-6.6 when the fermentation liquor is discharged from 12h to the tank, and co-culturing for 42-54h to obtain the fermentation liquor containing shikimic acid. Through double means of culture medium formula design, seed culture and fermentation culture process design, the conversion rate of synthesizing shikimic acid from glucose is synergistically increased, the overall operation is simple, the dosage of used methyl-alpha-D-glucopyranoside is small, the cost is low, more shikimic acid can be converted, and for medicine production enterprises, the method has a very good application prospect. The obvious cost-reducing and effect-increasing effects are realized.

The invention belongs to the technical field of food fermentation and functional drinks, and particularly relates to lactobacillus mucilaginosus and application thereof in preparation of fermented wolfberry juice for improving immunity. The lactobacillus mucus LFY21 strain is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the lactobacillus mucus LFY21 strain is CCTCC NO: M 20252569; the preservation address is Wuhan University. The strain grows vigorously in the Chinese wolfberry juice, the acid production capacity is weak in the Chinese wolfberry juice fermentation process, and the obtained Chinese wolfberry juice is high in saccharic acid content, good in flavor and palatability and high in free radical scavenging activity and total phenol content. In addition, animal experiments prove that the fermented Chinese wolfberry juice prepared from the strain can also generate the effect of effectively improving the immune function, and has the application potential as an immunopotentiator.