69 results about "Sugar acids" patented technology

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The invention discloses a nutrition regulating agent for regulating the mature period of citrus fruits and application of the nutrition regulating agent. The nutrition regulating agent is prepared from potassium nitrate, monopotassium phosphate, magnesium sulfate, ferric sodium ethylenediaminetetraacetate, zinc sodium ethylenediaminetetraacetate, boric acid, ammonium molybdate, gibberellin, abscisic acid and diatomite which is supplemented to 100 parts. The invention further provides a preparation method of the nutrition regulating agent and application of the nutrition regulating agent in regulating the mature period of citrus fruits, and the application mode is that when the diameter of the citrus fruits is 40-50 mm, the preparation is diluted by 600-800 times to be sprayed on leaf surfaces, once every 7 days and twice continuously. Through the synergistic effect of mineral nutrient elements and low-concentration hormones, bidirectional precise regulation and control of the citrus mature period are achieved, fruits can be promoted to mature in advance or later for 7-15 days, the sugar-acid ratio and color of the fruits can be remarkably improved, meanwhile, the problems of tree vigor decline and quality decline caused by single hormone treatment are solved, and the method is suitable for large-scale popularization and application. The method has the characteristics of high efficiency, safety and simple operation.

The application provides a compound microbial fertilizer, a preparation method and application thereof. The compound microbial fertilizer comprises the following components in parts by weight: 10-30 parts of a compound microbial agent, 20-50 parts of an agent carrier, 30-50 parts of a compound fertilizer slow-release agent and 10-30 parts of modified urea aldehyde. The compound microbial fertilizer prepared by the application can be applied to growth tests of tomatoes, and higher yield, stem thickness growth value, vitamin C content and sugar acid ratio can be obtained. The compound microbial fertilizer has long-term effect, can release phosphorus, nitrogen and potassium and the like, has the effects of moisturizing and loosening soil, can increase the yield and quality of tomato fruits, promote the robustness of roots and stems and improve the eating taste.

Provided is a composition for inhibiting the proliferation of bacteria belonging to the genus Collinsella, the composition containing at least one selected from sugar acid-containing oligosaccharides and salts thereof. The sugar acid is preferably selected from aldonic acid, uronic acid, and aldaric acid.

According to the method for improving the flavor of the Huangguo pear juice beverage, provided by the invention, the Huangguo pear residues can be effectively utilized to extract aroma components, and the original fruit flavor of the Huangguo pear juice beverage is greatly improved through scientific process steps such as composite enzymolysis, reasonable adjustment of the sugar-acid ratio and strict sterilization treatment; the loss of aroma substances is reduced, so that the product has higher typicality and high quality, and a new technical support and a new development direction are brought to the processing industry of the Huangguo pears in the same kernel region.

Aiming at a lactic acid return loss phenomenon existing in a process for producing lactic acid by microbial fermentation, the invention firstly provides application of a target gene in inhibiting lactic acid return loss in lactic acid production by a microbial fermentation method, and the target gene is a lactic acid permease gene lutP and / or an iron-sulfur oxidase gene cluster lutABC. On the basis of the application, the invention also provides a method for inhibiting lactic acid return consumption in lactic acid production by a microbial fermentation method, and a bacillus coagulans engineering strain with low lactic acid return consumption and high sugar-acid conversion rate. According to the method, effective inhibition of intracellular transport and / or metabolism of lactic acid is realized, so that the problem of lactic acid return consumption in a process for producing lactic acid through microbial fermentation is solved, and the technical effect is remarkable. According to the engineering strain, a LutR palindromic sequence in a lactic acid permease gene lutP promoter region is modified, and / or an iron-sulfur oxidase gene cluster lutABC is inactivated, so that transmembrane transport and / or intracellular metabolism of lactic acid are / is effectively inhibited, a lactic acid return loss phenomenon is remarkably weakened, the sugar-acid conversion efficiency is improved, the fermentation process flow is simplified, the production cost is reduced, and the engineering strain is suitable for industrial production. And the method has important value for large-scale production.

The invention relates to flavored fruit vinegar and a preparation method thereof, and the flavored fruit vinegar has the beneficial effects that the coexistence of flavor and nutrition is realized through the matching of apples and roxburgh roses; wherein the apple juice is used as a fermentation substrate, so that stable sugar-acid ratio and mellow feeling are provided; the rosa roxburghii tratt greatly enriches active ingredients such as vitamin C, SOD, polyphenol and the like, and lays a health-care foundation of the product. In the fermentation process, the synergistic effect of saccharomycetes, acetic bacteria and lactic acid bacteria is adopted, the saccharomycetes efficiently complete alcoholic fermentation, the acetic bacteria dominate acid production, the lactic acid bacteria not only can generate soft lactic acid, more importantly, metabolites of the lactic acid bacteria can effectively complex and convert tannin astringency in the roxburgh roses, and the taste is fundamentally improved. The final product successfully integrates the mellowness of the apples and the high nutrition of the roxburgh roses and is free of obvious bitter and astringent taste, and the taste, flavor and nutritional value are comprehensively improved.

The present invention provides a tegoprazan sugar acid salt and a preparation method therefor, the salt being pharmaceutically usable for gastroesophageal disease, gastroesophageal reflux disease, gastritis, peptic ulcer, gastric ulcer, duodenal ulcer, NSAID-induced ulcer, non-erosive reflux disease (NERD) and the like. The tegoprazan sugar acid salt of the present invention has excellent water solubility and stability of tegoprazan, and thus can be particularly useful as a pharmaceutical injection preparation.

The application discloses a kind of high-yield O-succinyl-L-homoserine recombinant escherichia coli and its construction method and application, belong to genetic engineering and fermentation engineering technical field.The recombinant escherichia coli is expressed by expressing anti-feedback inhibition homoserine O-succinyltransferase metA fbr , aspartate ammonia-lyase aspA, aspartate transaminase aspC, 2 ketoglutarate decarboxylase sucA, dihydrothioctic acid succinyltransferase sucB and efflux protein yjeH, knock out metJ, improve intracellular NADPH availability, to enhance OSH synthesis pathway metabolic flow, precursor succinyl coenzyme A supply and product efflux.Using 5 L bioreactor for fed-batch fermentation, after fermentation 60 h, OSH production is as high as 137.4 g / L, and sugar acid conversion rate reaches 50.5%.The application provides engineering strain and technical realization path for the efficient fermentation of OSH.

Various processes for preparing C4 aldoses and / or ketones thereof are described. Various processes are described for preparing C4 aldoses and / or ketones thereof from feed compositions comprising glycolaldehyde. Also, various processes for preparing useful downstream products and intermediates, such as erythritol and erythronic acid, from the C4 aldoses and / or ketones thereof are described.

The present application relates to the technical field of genetic engineering, and particularly relates to a recombinant microorganism, a preparation method thereof and application of the recombinant microorganism in threonine production. Expression of tpx gene and / or yodD gene in the recombinant microorganism is inhibited. The present application finds that inhibition of tpx gene or yodD gene in a strain can effectively improve the ability of the strain to produce amino acids, and the threonine production capacity of Escherichia coli with tpx gene and yodD gene knocked out can reach 27.35 g / L, and the sugar acid conversion rate can reach 27.94%, which has important significance in the field of threonine production.

The application discloses a method for producing lysine by immobilized continuous fermentation, and the method comprises the following steps: soaking a multi-hydroxy flexible polyester fiber carrier in soluble pectin, and drying the carrier by a dry heat method to obtain an immobilized carrier; inoculating seed liquid of cultured Corynebacterium glutamicum into a high-oxygen-bio-reactor containing the immobilized carrier and a fermentation medium, and performing batch-fed fermentation for a certain batch to immobilize the Corynebacterium glutamicum on the immobilized carrier, so that the Corynebacterium glutamicum can stably produce lysine; and switching to a mode for continuously producing lysine. The method for producing lysine by the immobilized Corynebacterium glutamicum fermentation can be stably operated for more than 15 days, and compared with free fermentation, the lysine production rate is increased by nearly 35%, and the sugar-acid conversion rate is increased by nearly 4 percentage points.

The application belongs to the technical field of biology, and discloses a strain for efficiently fixing carbon dioxide and improving succinic acid conversion rate and a construction method thereof. The application discloses a genetically engineered bacterium, which is obtained by overexpressing carbonic anhydrase genes, phosphoenolpyruvate carboxylase genes and phosphoenolpyruvate carboxykinase genes in a succinic acid-producing strain. ‑ The carbonic anhydrase can enhance the conversion efficiency of CO2 to HCO3 , and the phosphoenolpyruvate carboxylase and the phosphoenolpyruvate carboxykinase can realize efficient fixation of C3 and CO2 into C4 intermediates. Experiments prove that the genetically engineered bacterium can effectively produce succinic acid by using glucose, and has a relatively high sugar acid conversion rate (up to 79.80%).

The invention belongs to the technical field of food fermentation and functional drinks, and particularly relates to lactobacillus mucilaginosus and application thereof in preparation of fermented wolfberry juice for improving immunity. The lactobacillus mucus LFY21 strain is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the lactobacillus mucus LFY21 strain is CCTCC NO: M 20252569; the preservation address is Wuhan University. The strain grows vigorously in the Chinese wolfberry juice, the acid production capacity is weak in the Chinese wolfberry juice fermentation process, and the obtained Chinese wolfberry juice is high in saccharic acid content, good in flavor and palatability and high in free radical scavenging activity and total phenol content. In addition, animal experiments prove that the fermented Chinese wolfberry juice prepared from the strain can also generate the effect of effectively improving the immune function, and has the application potential as an immunopotentiator.

The invention provides mulberry wine obtained through microbial synergistic fermentation and distillation and a preparation method thereof, and relates to the technical field of fruit wine brewing and processing. Comprising the following steps: preparing mulberry juice, regulating and controlling the sugar-acid ratio of the mulberry juice, brewing the mulberry juice, performing low-temperature distillation, and blending and filling wine liquid, namely regulating and controlling the sugar-acid ratio of the mulberry juice by adopting white granulated sugar and citric acid. The sugar degree of the mulberry juice subjected to enzymolysis is adjusted to 150-270 g / L, and the pH value is adjusted to 3.1-3.9, so that other sterilization treatment, low-temperature distillation and staged wine liquid retention do not need to be performed in the mulberry juice, and the aromatic substances in the mulberry juice and the taste of the finished wine liquid are increased. The invention provides the mulberry wine which is prepared by synergistically fermenting and distilling microorganisms and is good in taste and strong in fragrance and the preparation method of the mulberry wine.

This invention relates to a method for preparing polylactic acid (PLA) by straw fermentation coupled with lactide ring-opening polymerization, belonging to the field of biomass resource utilization and bio-based polymer materials technology. To address the problems of high energy consumption and low sugar conversion rate in existing PLA preparation methods using crop straw, this invention provides a method for preparing PLA by straw fermentation coupled with lactide ring-opening polymerization. The method involves crushing the straw, pre-treating it with dilute acid coupled with steam explosion, washing and drying it, adding a complex cellulase and Lactobacillus pentosus LB-1 seed culture, and simultaneously saccharifying and fermenting under controlled temperature and pH to obtain a lactic acid fermentation broth. The fermentation broth undergoes multi-stage filtration, ion exchange, and concentration to obtain refined lactic acid, which is then prepolymerized, depolymerized, and distilled to obtain L-lactide. After purification, an initiator and antioxidant are added, and ring-opening polymerization, extraction, and drying are performed under nitrogen protection to obtain pure PLA. This invention features efficient and low-energy straw pretreatment, high sugar-acid conversion rate during fermentation, precise purification, stable polymerization, and low cost, making it a green and economical method using straw as raw material.

The invention belongs to the technical field of fertilizers, and particularly relates to a fulvic acid fertilizer for improving passion fruit quality and amino acid composition and application of the fulvic acid fertilizer. A preparation method of the fulvic acid fertilizer for improving the passion fruit quality and the amino acid composition comprises the following steps: S1, preparing a basic solution: mixing a potassium fulvate mother solution with deionized water to form the basic solution; s2, constructing an electro-Fenton system; s3, electro-Fenton activation reaction: applying constant current to an electro-Fenton reaction system, quenching after reaction, and adjusting the pH value; s4, carrying out chelation reaction; and S5, fixing the volume. The fulvic acid fertilizer disclosed by the invention can regulate and control secondary metabolism of crops, can remarkably improve the soluble solid content of fruits, reduces total acid, optimizes the sugar-acid ratio, and enables the flavor of the fruits to be better; meanwhile, the amino acid composition of fruits can be systematically improved, and the total amount of amino acids, especially the content of arginine, leucine and other key amino acids, can be remarkably increased.

This invention discloses a genetically engineered bacterium producing high levels of L-homoserine, its construction method, and its applications, belonging to the field of biotechnology. The genetically engineered bacterium uses *Escherichia coli* W3110 as the substrate cell. By knocking out the metA, lysA, thrBC, pflB, ldhA, poxB, adhE, iclR, sthA, tdcC, lacI, and ptsG genes, and replacing the promoters of ppc, aspC, and gdhA genes with the inducible strong promoter Ptrc, and by performing site-directed mutagenesis on the thrA and asd genes, and simultaneously overexpressing the thrA*, pnhsd, asd, asd*, and rhtA genes using free plasmids, a recombinant strain HOM20 was constructed. This strain, fermented in a 5 L fermenter for 48 h, achieved an L-homoserine yield of 135.4 g / L and a sugar-acid conversion rate of 44%, significantly superior to existing technologies. This invention achieves directional carbon flow allocation, cofactor balance, feedback inhibition removal, and enhanced product efflux through multi-gene synergistic modification, and has broad prospects for industrial application.

To provide an alcoholic beverage which does not have excessive sour taste and sweet taste and to which robust drinking feeling is imparted SOLUTION: An alcoholic beverage comprising sweet components, sour components, and α - pinene or β - pinene, and having an extract content of 3.0 degrees or less, wherein the sugar-acid ratio is 2 to 18, and the total content of α - pinene and β - pinene relative to the total amount of the alcoholic beverage is 0. 1ppm or more.SELECTED DRAWING: None

The invention discloses a wolfberry-astragalus compound polysaccharide with an anti-myelosuppression effect as well as a preparation method and application of the wolfberry-astragalus compound polysaccharide. The wolfberry-astragalus compound polysaccharide is prepared by adopting a process of separately extracting wolfberry polysaccharide and astragalus polysaccharide and then mixing, the total sugar content is 70-80%, the uronic acid content is 10-14%, and the wolfberry-astragalus compound polysaccharide has specific molecular weight distribution and monosaccharide composition characteristics. Animal experiment results show that the wolfberry and astragalus compound polysaccharide can significantly improve peripheral hemopenia of myelosuppression mice induced by cyclophosphamide, promote recovery of the number of leukocytes, lymphocytes, monocytes and neutrophils, and effectively reverse weight loss caused by chemotherapeutic drugs. The wolfberry-astragalus compound polysaccharide provided by the invention is high in safety and remarkable in anti-myelosuppression effect, and can be used for preparing medicines or functional preparations for improving myelosuppression related diseases.

The present invention relates to conjugates comprising a polysaccharide comprising a 3-deoxy-D-mannan-octanone acid (KDO) moiety, in particular conjugates produced using a random conjugation process, processes for preparing such conjugates, immunogenic compositions and vaccines comprising the conjugates, and therapeutic methods or medical uses using the compositions and vaccines.

The invention discloses a fermentation culture medium for improving the fermentation production stability of arginine and a culture process. In the fermentation culture medium, the molasses dosage is reduced, so that the raw material cost and the component fluctuation risk can be reduced; the yeast extract powder and the molasses have a synergistic effect, so that various amino acids and vitamins can be quickly utilized for thallus growth, the negative effect of fluctuation of nutritional ingredients of the molasses is counteracted, and the sugar-acid conversion rate is increased. In the culture process, through two-stage pH control, the thallus growth and product synthesis environment can be optimized, and the process stability is improved; through linkage regulation and control of dissolved oxygen and material supplementation, sudden drop of dissolved oxygen caused by excessive accumulation of substrates can be avoided, energy consumption is reduced, and process control precision is improved; the method is suitable for fermentation systems of different scales and facilitates industrial application.

This invention discloses a fermentation method for L-lysine, belonging to the field of L-lysine fermentation technology. The fermentation method involves inoculating a secondary *Corynebacterium glutamicum* seed culture at an inoculation rate of 9.8-10.2% into a fermenter containing 1L of fermentation medium. The temperature inside the fermenter is maintained at 34-36℃, the pressure at 0.07-0.08 MPa, and the dissolved oxygen at 20-25%. The pH is maintained at 7-7.2 by adding ammonia. When the residual sugar decreases to 2g / L, a glucose aqueous solution is added to control the residual sugar to 5-8g / L. After 8 hours of fermentation, a betaine supplement is added. After 10 hours of fermentation, a culture medium supplement is added. This fermentation method can improve the L-lysine production rate, L-lysine yield, and sugar-acid conversion rate of *Corynebacterium glutamicum*.

We provide an engineered fungus capable of increasing tryptophan yield, as well as methods for its construction and use. Through screening, we isolated a strain capable of tolerating high concentrations of tryptophan. After analyzing its genome and protein sequences, we discovered that point mutations occurred in several proteins in the strain, and these mutants were able to increase tryptophan yield. To further increase tryptophan yield, we modified the protein sequence expressed by the fadR gene or the protein sequence expressed by the pepD gene in the starting strain. Through these modifications, the resulting engineered fungus exhibited a higher tryptophan yield than the starting strain. In large-scale production, the tryptophan yield in a 5L fermenter reached 62.38±5.80 g / L, with a sugar acid conversion rate of 24.1%. Compared to the original strain, the tryptophan yield increased 1.48-fold and the sugar acid conversion rate increased 1.26-fold. The above biomaterials and their uses belong to the field of molecular biology and have broad practical value.

The invention belongs to the technical field of tobacco processing, and relates to a tobacco leaf quality adding and improving method which includes the steps that 8 g of essence is added to every 100 g of tobacco leaves, and the essence formula comprises, by mass, 0-0.3 g of licorice powder, 0-0.3 g of honey, 0-0.1 g of an anglersugar acid material, 0-0.5 g of a conventional sugar acid material, 0-0.3 g of J-MRP, 0-0.1 g of JX13, 0-1 g of Y-BRP, 0-0.5 g of 99125-3, 0-0.05 g of a sweet aroma base, 0-0.1 g of malt extract, 0-0.05 g of a flavor enhancing base, 0-1 g of G9901 and 6.01-7.1 g of water; the purity of the licorice powder is 5%, and the purity of the honey is 50%; through compatibility research on Shaanxi angkang tobacco leaves and provincial Yuluo tobacco leaves, a proper combination proportion is determined; a multi-batch feeding formula is designed and screened out, and the feeding effect is continuously optimized; the feeding and cut tobacco drying conditions are subjected to laboratory experiment optimization, and the suitable cut tobacco drying strength of different modules is determined.

This application relates to the fields of bioengineering and fermentation technology, and discloses a genetically engineered *Escherichia coli* strain that synthesizes L-histidine and its preparation method. The engineered strain uses *Escherichia coli* W3110 as the starting strain and is modified... hisG Gene release from feedback inhibition; knockout ushA and nrdD Genes reduce precursor consumption; inactivation slyA Genes reduce energy waste; heterogeneous integration zwf and gnd Genes and replacement pgi The gene promoter redirects carbon flow to the pentose phosphate pathway, enhancing the supply of key precursors PRPP and reducing agent NADPH; introducing courtE Genes promote product efflux. Combined with an optimized fed-batch fermentation process, this invention effectively solves the problems of insufficient precursor supply, uneven energy distribution, and product inhibition through a systems metabolic engineering strategy, increasing L-histidine yield and sugar-acid conversion rate, making it suitable for industrial production.