A kind of microbial fermentation process to produce arginine

A microbial fermentation and arginine technology, applied in the direction of microorganisms, microorganism-based methods, fermentation, etc., can solve the problems of product quality insecurity, labor and time-consuming, low yield, etc., to reduce product damage and improve filtrate quality , Improve the effect of product yield

Inactive Publication Date: 2015-09-23
滨州市生物技术研究院有限责任公司
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AI Technical Summary

Problems solved by technology

The fermentation liquid in the biological fermentation process contains a large amount of organic and inorganic impurities. The traditional post-extraction treatment process does not guarantee the quality of the product, and it is labor-intensive and time-consuming
The prior art uses plate and frame filtration during extraction, which takes a long time, low yield and large loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0030] The preparation of fermented liquid comprises the following steps:

[0031] (1) Slant culture: insert the MQA121 strain into the sterilized slant medium (formula glucose 2.0-2.5%, (NH 4 ) 2 SO 4 0.5-0.8%, KH 2 PO 4 0.1-0.3%, K 2 HPO 4 ·3H 2 O0.3-0.6%, MgSO 4 ·7H 2 O0.05-0.1%, FeSO 4 ·7H 2 O 0.002-0.01%, MnSO 4 ·H 2 O0.002-0.008%, agar powder 2.0%, pH7.0~7.4, cultivated at 28°C, and obtained slant bacteria for 28 hours;

[0032] (2) Shake flask strain: put the cultured slant strain into the shake flask sterilized seed medium and cultivate at 28°C for 22 hours to obtain the shake flask seed liquid;

[0033] (3) First-level seed culture: put the cultivated shake flask seed solution into the sterilized first-level seed medium, and cultivate it at 28°C for 14 hours to obtain the first-level seed solution;

[0034] (4) Secondary seed culture: put the cultivated primary seed liquid into the sterilized secondary seed medium and cultivate it at 28°C for 4 hours to ...

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Abstract

The invention discloses a process for producing arginine by microbial fermentation. Brevibacterium flavum MQA121 is adopted. The method comprises the following steps: preparing fermentation liquid, namely performing slant culture, performing shake-flask culture, performing primary seed culture, performing secondary seed culture, and continuing to ferment; extracting and refining a fermented product, namely preprocessing the fermentation liquid, performing secondary crystallization, performing color and impurity removal, performing membrane concentration and separation, drying, and packaging to obtain arginine as a finished product. The method has the benefits that organic and inorganic matters and bacteria in subsequent extracting and refining liquid of arginine can be remarkably reduced and the arginine yield can be increased; in addition, the product quality is improved and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of high-efficiency extraction and purification of amino acid fermentation liquid, in particular to a process for producing arginine by microbial fermentation. Background technique [0002] Arginine is widely used in medicine, nutrition and health care, food and other fields, and is in great demand. It is called one of the most important varieties of amino acids. L-arginine is an important intermediate metabolite in the urea cycle of organisms. All body tissues use L-arginine to synthesize cytoplasmic and nuclear proteins. In terms of physiological activity, in addition to being related to the induction of hormones such as growth hormone, insulin, and glucagon, it has also attracted attention as a vasodilation factor in recent years, and is expected to become a new material for nutritional therapy. Because L-arginine not only has important scientific research, actual production and application value, but als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/10C07C279/14C07C277/08C12R1/13
Inventor 闫洪波王威高树营
Owner 滨州市生物技术研究院有限责任公司
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