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Complex microbial inoculant, method for preparing same and application of complex microbial inoculant

A compound bacterial agent and microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of human-animal cross-infection, human health hazards, physical health hazards, etc., and achieve good composting effect. Clear and deodorizing effect

Inactive Publication Date: 2016-03-02
YANGZHOU KEHUI ECOLOGICAL ENG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Raising livestock and poultry directly without treatment will cause harm to human health through the accumulation of toxins and harmful substances in the livestock and poultry, resulting in cross-infection between humans and livestock
In addition, the "waste oil" sold by unscrupulous traders contains toxic substances such as aflatoxin and benzene, which return to people's dining tables through underground channels and are eaten by people, causing chronic diseases and even cancer, which is extremely harmful to people's health. big hazard

Method used

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  • Complex microbial inoculant, method for preparing same and application of complex microbial inoculant
  • Complex microbial inoculant, method for preparing same and application of complex microbial inoculant
  • Complex microbial inoculant, method for preparing same and application of complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 The preparation embodiment of microbial composite bacterial agent of the present invention

[0028] 1.1 The cultivation of various strains

[0029] This embodiment uses Saccharomyces cerevisiae CGMCC2.3886, Saccharomyces cerevisiae

[0030] Pseudomonas veronii CGMCC1.7760,

[0031] Pseudomonas simiensis PseudomonassimiaeCGMCC1.6458,

[0032] Brevibacterium epidermidisCGMCC1.10889,

[0033] Bacillus cereus CGMCC1.10559,

[0034] Paenibacillus polymyxaCGMCC1.4261,

[0035] Paeni Bacillus amylolyticus CGMCC1.3460,

[0036] The microbial composite bacterial agent of the present invention was prepared as a strain, and the above strains were all purchased from the Institute of Microbiology, Chinese Academy of Sciences. Using the above-mentioned strains, inoculate and culture them separately in the medium, which is mixed with 2g of peptone, 20g of glucose, 2g of sodium chloride, and 30g of potato flour per 1 liter of water; the culture conditions are 30°C fo...

Embodiment 2

[0043] Embodiment 2 The waste degradation rate test of compound microbial strain of the present invention

[0044] 1 Experimental materials

[0045] Microbial strains prepared in Example 1 and composite thalli;

[0046] Kitchen waste: collected in the No. 1, No. 2 and No. 3 canteens of Beijing Forestry University;

[0047] Experimental instruments and equipment:

[0048] YP6001 electronic balance (Shanghai Yueping Scientific Instrument Co., Ltd.), GC310 gas detector (Beijing Zhongxi Yuanda Technology Co., Ltd.), TM-767II mixer (Zhongshan Haipan Electric Co., Ltd.), triangular flask, 751-type ultraviolet spectrophotometer Wait.

[0049] The 7 strains fermented and cultured in Example 1.1 were respectively labeled No. 1 to No. 7, the composite bacterial body prepared in Example 1.1 was labeled No. 8, and the blank control was No. 0.

[0050] 2. Comparison of kitchen waste reduction

[0051] Put the sorted kitchen waste into a blender and grind it, and add wood chips to adju...

Embodiment 3

[0057] Example 3 Comparison experiment of the treatment effect of the composite bacterial agent of the present invention and other commercial bacterial agents on kitchen waste

[0058] 1. Materials and methods

[0059] 1.1 Experimental materials

[0060] (1) Microbial agent

[0061] A: the composite bacterial agent prepared in Example 1; B: China Taiwan "Tiangeng" bacterial agent (produced by Fugeng Ecological Co., Ltd.); C: "Yilezai" bacterial agent (Hangzhou Lvjian Agricultural Leisure Co., Ltd.); D: Japanese EM bacterial agent (Beijing Kangyuan Oasis Biotechnology Co., Ltd. Production);

[0062] (2) kitchen waste and experimental instrument and equipment are the same as embodiment 2

[0063] 2. Reduction experiment

[0064] Reduction is one of the most intuitive indicators to measure the degradation effect of kitchen waste. Only reduction can reduce the amount of waste discharged into the environment, thereby alleviating the burden of subsequent waste management and r...

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Abstract

The invention relates to a complex microbial inoculant. The complex microbial inoculant comprises eleven types of complex inoculants and accessories. The complex inoculants comprise seven types of bacteria such as saccharomyces cerevisiae, pseudomonas veronii and brevibacterium epidermidis and are added to the accessories, and the complex inoculants and the accessories are subjected to solid fermentation to obtain the complex microbial inoculant. The complex microbial inoculant has the advantages that kitchen waste can be effectively reduced by the complex microbial inoculant as compared with other commercially available inoculants, odor generated by the kitchen waste in compost maturity procedures can be effectively reduced, and the compost maturity level can be upgraded.

Description

technical field [0001] The invention relates to a microbial composite bacterial agent, in particular to a microbial composite bacterial agent for decomposing kitchen waste, as well as its preparation method and application. Background technique [0002] The main components of kitchen waste include rice and flour food residues, vegetables, meat and bones, etc. From the chemical composition, there are starch, cellulose, protein, lipids and inorganic salts. The main features are rich in organic matter, high in water content, and perishable. Its properties and odor will have a bad impact on environmental sanitation, and it is easy to breed harmful substances such as pathogenic microorganisms and mycotoxins. [0003] Due to food culture and eating habits, China produces a huge amount of kitchen waste every day. It is estimated that Chinese cities generate no less than 60 million tons of kitchen waste every year. Nutritious kitchen waste is a valuable renewable resource. Howeve...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C05F9/04C05F11/08C12R1/01C12R1/13C12R1/085C12R1/38C12R1/865
CPCY02A40/20
Inventor 徐锐周传斌陈永根
Owner YANGZHOU KEHUI ECOLOGICAL ENG TECH CO LTD
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