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Pycnoporus sp. Strain YZC9 and application thereof

A technology of dense porosity bacteria and strains, applied in the field of microorganisms, can solve the problems that the strains of dense pores bacteria have not been reported, and achieve good composting effect and good application prospects

Active Publication Date: 2019-02-15
BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report in the field of the Mypoporia strains possessing both cellulose and lignin degradation ability

Method used

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  • Pycnoporus sp. Strain YZC9 and application thereof
  • Pycnoporus sp. Strain YZC9 and application thereof
  • Pycnoporus sp. Strain YZC9 and application thereof

Examples

Experimental program
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preparation example Construction

[0083] The embodiment of this group provides a kind of preparation method of compost. All the examples in this group have the following common features: the preparation method includes: inoculating the compost material with the Pycnoporus sp. strain YZC9 with the preservation number CGMCC No. 14783 during the composting process.

[0084] In some embodiments, the compost material includes garden waste; the garden waste is selected from: animal manure, litter, mushroom residue;

[0085] In a further embodiment, the garden waste is preferably a mixture of cow dung and mushroom dregs in a volume ratio of 8:2;

[0086] In a specific embodiment, the compost material is inoculated with the Pycnoporus sp. strain YZC9 at an inoculation rate of 0.5% by volume.

experiment example

[0087] Experimental example, experimental operation process of the present invention

[0088] 3 Experimental methods

[0089] 3.1 Isolation of soil strains

[0090] Prepare sterile water, press the soil sample by 10 -3 to 10 -7 Dissolve the concentration gradient in sterile water and fully dissolve. Then pipette the mixture and inoculate it on PDA medium, and culture it at 28°C for 5-7 days.

[0091] 3.2 Species identification and purification

[0092] The fungi, bacteria and actinomycetes were preliminarily determined according to the morphology, and were connected to their respective special mediums, inoculated and purified repeatedly until pure strains were obtained, and set aside.

[0093] 3.3 Screening method of lignin-degrading bacteria

[0094] 3.3.1 PDA-guaiacol plate color reaction

[0095] Inoculate the purified strains on the PDA-guaiacol medium plate, culture at 28°C for 5 days, record whether there is a reddish-brown discoloration circle and the size of the...

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Abstract

The invention discloses Pycnoporus sp. strain YZC9 and application thereof and belongs to the technical field of microorganisms. The preservation number of the Pycnoporus sp. strain YZC9 is CGMCC No.14783. Based on the strain, the invention also provides a degraded lignin / cellulose inoculant containing the strain, a biodegradable agent, a product for composting, an additive as well as a lignin / cellulose degradation method using the strain, a preparation method of compost and a method for producing manganese peroxidase. The activity of the manganese peroxidase (MnP) of the YZC9 strain disclosedby the invention can reach as high as 116U / ml; meanwhile, the Pycnoporus sp. strain YZC9 also can obtain a good composting effect and has good application prospect of industrial production of the manganese peroxidase (MnP) and garden composting.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a Pycnoporus sp. strain YZC9 and its application. Background technique [0002] Pycnoporus sp. is a kind of fungus belonging to Polyporaceae Polyporaceae, basidiocarp 1-perennial, non-flat, basidiocarp sessile, cap corky; flesh orange-red, case 10% KOH turns black; mycelial system is triphyletic hyphae with cord-like associations; hymenium is tubular, sometimes with cystic bodies, without setae. Fruiting bodies are solitary, grouped or stacked. [0003] The discovery of new strains of the genus Mypoporia is rarely reported, and the functional strains of Myopora are even rarer. A Pycnoporus sanguineus strain mk528 capable of producing laccase has been reported in the prior art, and its preservation number is CGMCC NO.1124. Microporus haemophilus, a species of Polyporaceae, is a wood-dwelling, saprophytic small and medium-sized mushroom that grows in low- and middle-alt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/08C05F15/00C05F17/00C12R1/645
CPCC05F3/00C12N1/14C12N9/0065C12Y111/01013C05F17/20C12N1/145C12R2001/645C05F5/00C05F11/00Y02W30/40
Inventor 刘悦秋崔金腾李雅君于峰张秋月
Owner BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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