Neosporum apescifolia with antibacterial ability and lignocellulose degrading activity and its application

A technology of lignocellulose and Pantotheca, applied in fungi, fungicides, microorganism-based methods, etc., can solve the problem of not mentioning the bacteriostatic activity of Penicillium oxalicum, etc.

Active Publication Date: 2021-06-11
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this study only focused on cellulase, and paid less attention to other enzymes involved in lignocellulose degradation; the study did not mention whether Penicillium oxalicum has antibacterial activity

Method used

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  • Neosporum apescifolia with antibacterial ability and lignocellulose degrading activity and its application
  • Neosporum apescifolia with antibacterial ability and lignocellulose degrading activity and its application
  • Neosporum apescifolia with antibacterial ability and lignocellulose degrading activity and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, isolation and cultivation of bacterial strain M51-1

[0021] 1. Configuration of isolation and culture medium

[0022] MEA (separation) medium: Maltose extract (malt extract, Oxoid) 20g, dissolved in an appropriate amount of ultrapure water, added 15g of agar, and the volume of ultrapure water was adjusted to 1L. After sterilization, cool at room temperature to 45°C, and add streptomycin sulfate and tetracycline hydrochloride at final concentrations of 0.05g / L and 0.02g / L to inhibit bacterial contamination.

[0023] PDA (cultivation) medium: 200g of potatoes, peeled, cut into small pieces, boiled with ultrapure water, filtered with four layers of gauze, added with 20g of glucose, 15g of agar, and adjusted to 1L with ultrapure water. After sterilization, cool at room temperature to 45°C, and add streptomycin sulfate and tetracycline hydrochloride at final concentrations of 0.05g / L and 0.02g / L to inhibit bacterial contamination.

[0024] 2. Isolation and c...

Embodiment 2

[0028] Embodiment 2, molecular biological identification of bacterial strain M51-1

[0029] 1. Strain DNA extraction and PCR amplification

[0030] Pick the purely cultured fungal hyphae into a 1.5ml sterile centrifuge tube, and use the DNeasy Plant Mini Kit (Qiagen) kit to extract the fungal DNA. Using DNA as a template, the internally transcribed spacer sequence (ITS) was amplified, and the primers used were ITS1F: TCCGTAGGTGAACCTGCGG; ITS4: TCCTCCGCTTATTGATATGC.

[0031] A total of 25 μl of the amplification system: template DNA, 1 μl; forward and reverse primers, 0.5 μl each; Taq enzyme, 0.2 μl; dNTP, 2 μl; Buffer, 2.5 μl; sterile ultrapure water, 18.3 μl. The PCR amplification program included: 94°C pre-denaturation, 4min; 35 cycles, including 94°C pre-denaturation for 40s, 55°C annealing for 50s, 72°C extension for 1min; final extension for 10min.

[0032] 2. Sequence determination and species identification

[0033] After amplification and sequencing, the obtained IT...

Embodiment 3

[0037] Embodiment 3, the antibacterial ability of bacterial strain M51-1 (improves the resistance ability of plant to pathogenic bacteria)

[0038] The strain M51-1 (Pezicula neosporulosa) and the pathogen Fusarium oxysporum (Fusarium oxysporum) were inoculated on PDA plates respectively, and cultured at 25°C in the dark for 7 days.

[0039] Treatment group: pick a small amount of equal bacterial blocks of the two strains (about 0.5×0.5cm in size) in the PDA medium, respectively located at both ends of the diagonal of the plate and 1cm away from the edge; one end of the control group was inoculated with oxysporum Fusarium, with an agar block at the other end, cultured in the dark for 10 days. Measure the strain radius of the treatment group and the control group with a ruler, and calculate the bacteriostatic rate. like figure 1 As shown, the pathogenic bacteria colony of the treatment group (M51-1) was significantly smaller than that of the control group (control), indicatin...

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Abstract

The invention belongs to the field of biological technology, and in particular relates to a Pezicula neosporulosa M51‑1 strain and its activity. The invention discloses a Pezicula neosporulosa M51‑1, which is Pezicula neosporulosa, and the preservation number is CGMCC No: 18137. The present invention also discloses the application of the above-mentioned A. neosporum M51-1: for improving the resistance of plants to pathogenic bacteria and / or providing engineering bacteria for degrading lignocellulose.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an endophytic fungus Peziculaneosporulosa M51-1 strain and its activity. Background technique [0002] Plant endophytic fungi usually refer to fungal groups that can colonize roots, stems, leaves, flowers, seeds and other tissues and organs of plants, but do not cause obvious symptoms to the host. As the importance of the symbiotic relationship between fungi and plants is continuously revealed, more and more scientists agree that plants are actually a "plant-symbiotic" life community. As an important component of the plant root microbiome, plant endophytic fungi have received extensive attention and research on their diversity in composition and function. In terms of composition, endophytic fungi mainly consist of Ascomycetes, Basidiomycetes, and Glomeromycetes. In terms of function, endophytic fungi can directly or indirectly affect plants through a variety of ways, su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 杨预展袁志林王欣玉
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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