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Enterococcus azikeevi EO-07 and application thereof in ethylene oxide degradation

A technology of ethylene oxide and enterococcus, applied in the direction of bacteria, microorganism-based methods, methods using microorganisms, etc., can solve the problems of high biological toxicity, complex microenvironment of microbial degradation pool, and influence

Inactive Publication Date: 2020-05-12
CHINESE RES ACAD OF ENVIRONMENTAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two main methods for industrial sterilization treatment of ethylene oxide: one is to use sulfuric acid as the reaction substrate for neutralization reaction, the disadvantage of this technology is that the absorption saturation is small, and it will produce side effects that are difficult to handle. Product increases processing costs
The other is to use an oxidation reaction furnace for oxidation reaction. This technology requires very high control of technical parameters, and the process is prone to explosion.
[0004] Microbial degradation is a possible solution. However, as a fungicide, ethylene oxide is highly toxic to organisms, and the microenvironment of the microbial degradation pool is very complex and affected by many factors.

Method used

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  • Enterococcus azikeevi EO-07 and application thereof in ethylene oxide degradation
  • Enterococcus azikeevi EO-07 and application thereof in ethylene oxide degradation
  • Enterococcus azikeevi EO-07 and application thereof in ethylene oxide degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Enrichment, screening and identification of EO-07 original strain

[0069] Weigh 10.0 g of sludge sample, add 100 mL of 0.03 mol / L phosphate buffer, mix and clarify for 120 min, and remove large particles of sediment. Take 1mL of the suspension and add 10mL of enrichment enrichment liquid medium (containing 100mg / L low concentration of ethylene oxide), put it on a shaker for oxygen consumption enrichment culture at 200r / min, and incubate at 37°C for 24-48h Observe the growth.

[0070]This example enrichment enrichment liquid medium (Sabouraud liquid medium): Glucose 40g, casein trypsin digest, gastric enzyme digest of animal tissue equal mix 10g, pH5.4~5.8, distilled water 1000mL, aliquot to 500mL Erlenmeyer flasks, 250mL for each Erlenmeyer flask. Sterilize at 121°C for 20 minutes and cool to room temperature. Take out the ice box from the refrigerator, and place the reagent pure ethylene oxide on the ice box. Use a sealed syringe to draw 28 μL of ethylene oxide li...

Embodiment 2

[0086] Ethylene oxide-induced domestication of EO-07 original strain

[0087] Ethylene oxide tolerance-induced acclimatization: using the streaking method, inoculate EO-07 original strains in a 100mg / L ethylene oxide-tolerant acclimatization medium, and culture at a constant temperature of 37°C for 48 hours; select the one with the largest colony radius on the plate Single colony, subculture to 200mg / L ethylene oxide tolerance acclimatization medium, 37 ℃ constant temperature culture for 48h; select the single colony with the largest colony radius on the plate, subculture to 500mg / L ethylene oxide tolerance and acclimatization culture medium at a constant temperature of 37°C for 48 hours; select the single colony with the largest colony radius on the plate, subculture to a tolerance acclimatization medium containing 800 mg / L ethylene oxide, and cultivate at a constant temperature of 37°C for 48 hours to obtain high-concentration ethylene oxide tolerance Receptor species.

[0...

Embodiment 3

[0094] Identification of Ethylene Oxide Degradation Effect of EO-07 Strains

[0095] Microbial culture and activation: Take out EO-07 strains and EO-07 original strains from the -80°C refrigerator, take 10 μL and inoculate them into 100 mL Sabouraud liquid medium, culture at 37°C, 200 rpm, for 48 hours. The number of bacteria in the culture solution is 10 10 ~10 12 cfu / mL.

[0096] Sabouraud liquid medium: Glucose 40g, casein trypsin digest, animal tissue gastric enzyme digest equal mixture 10g, pH5.4~5.8, distilled water 1000mL, divided into 500mL Erlenmeyer flasks, each Erlenmeyer flask 250mL. Sterilize at 121°C for 20 minutes and cool to room temperature.

[0097] Sabouraud's liquid induction medium: 10g of peptone, 1000mL of distilled water, aliquoted into 400mL, sterilized at 121°C for 20min, cooled to room temperature for storage. Before use, add 160 mg of ethylene oxide with a sealed syringe to make a liquid medium containing no carbon source but containing 400 mg / ...

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Abstract

The invention discloses Enterococcus azikeevi EO-07 and application thereof in ethylene oxide degradation, and relates to the technical field of biodegradation in ethylene oxide treatment technologies. The preservation number of the Enterococcus azikeevi EO-07 is CGMCC No. 18437. The invention further discloses application of the Enterococcus azikeevi EO-07. By the Enterococcus azikeevi EO-07 andthe application thereof in ethylene oxide degradation, the sewage treatment and discharge problems in industrial ethylene oxide aftertreatment measures are solved, microorganisms for ethylene oxide degradation are screened out effectively, and the degradation effect of degrading bacteria is improved. The EO-07 can degrade ethylene oxide with the concentration of 400 mg / L under the condition of nocarbon source, and the ethylene oxide degradation rate can reach 65.42%; and the ethylene oxide with high concentration of 800 mg / L can be degraded under the condition of no carbon source, and the ethylene oxide degradation rate can reach 50.22%.

Description

technical field [0001] The invention relates to the technical field of biodegradation in ethylene oxide treatment technology, in particular to the screening and application of ethylene oxide degrading bacteria dissolved in liquid, more specifically to a kind of enterococcus EO-07 and its Applications for the degradation of ethylene oxide. Background technique [0002] At present, ethylene oxide is one of the important petrochemical products in the modern chemical industry. Ethylene oxide has an irreplaceable position in the medical sterilization industry due to its small molecular weight, high penetrability, and its ability to undergo coupling reactions with biological macromolecules such as DNA and proteins. In addition, the cost of ethylene oxide sterilization is low, and it can be used for industrial-scale large-scale sterilization. It is one of the most important low-temperature sterilizers so far. However, ethylene oxide is extremely active, flammable and explosive, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01C02F101/34
CPCC02F3/34C02F2101/34C12N1/36C12N1/205C12R2001/46
Inventor 薛建龙刘征涛侯东新武健王晓南韦继勇曾育平蒋丽娟
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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