Rhodotorula mucilaginosa for degrading penicillin as well as application thereof

A technology of Rhodotorula marcescens and penicillin, applied in the field of microorganisms, can solve the problems of not much, low concentration of penicillin, low efficiency of penicillin degradation, etc., and achieve high degradation effect

Active Publication Date: 2018-09-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, although there are examples of screening bacterial strains capable of degrading penicillin, there are not many studies at home and abroad, and there are problems

Method used

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  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof
  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof
  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1: Screening of strains

[0027] Weigh 10g each of the sample soil, put them into an Erlenmeyer flask containing 100ml sterile and a layer of glass beads, shake the shaker for 10 minutes, fully break it up, and make 10 -1 g·L -1 Bacteria suspension, diluted 10 times with distilled water to 10 -5 g·L -1 , 10 -6 g·L -1 , 10 -7 g·L -1 Concentration, the three concentrations of bacterial suspension were applied to the pre-prepared YPD medium containing penicillin at a concentration of 12 g / L, and the coating amount was 100 μL. Placed in a 30°C constant temperature incubator for cultivation. After 24 hours of culture, the grown strain was transferred to YPD medium with a concentration of 20g / L penicillin, and then subjected to multiple isolation and purification operations to obtain a single colony strain named Anti-Pen20.

[0028] Such as figure 1 As shown, under the transmission electron microscope, the cells are oval, without flagella, and budding. Using the yeast gen...

Example Embodiment

[0029] Example 2: Physiological and biochemical study of strains

[0030] (1) Optimum growth temperature experiment

[0031] The strains were inoculated on YPD medium and placed in a constant temperature incubator at 25°C, 30°C, 37°C, and 40°C for culturing for 1-2 days. Observe and record the growth of each strain. It is known that the optimal growth temperature range of the strain is 25-30°C.

[0032] (2) Optimal growth pH experiment

[0033] Prepare YPD medium with pH values ​​of 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, adjust the pH with HCl solution and NaOH solution, and measure the pH with a pH meter. The strains were inoculated on starch media with different pH values ​​and cultured in a constant temperature incubator at 30°C for 1-2 days. Observe and record the growth of each strain. It is known that the optimal growth pH range of the strain is 4.0-7.0.

[0034] (3) Growth of strains

[0035] Strain under the condition of testing medium: KH 2 PO 3 3g / L, K 2 HPO 3 1g / L, NH 3 NO 3 0.5g...

Example Embodiment

[0046] Example 3: Detection of penicillin concentration in fermentation broth

[0047] Set up 3 groups of detection medium, the first group adds penicillin to the detection medium to make the concentration 20g / L without adding the bacterial solution, the second group adds penicillin to the detection medium to make the concentration 20g / L and inoculate 10 % Anti-Pen20 bacterial solution, the third group was directly inoculated with 10% Anti-Pen20 bacterial solution in the test medium without adding penicillin. After culturing in a constant temperature shaker at 30°C, the fermentation broth was taken for processing and passed through HPLC The chromatograph analyzes the fermentation broth, using penicillin standard solution (0.5g / L, 1g / L, 2g / L, 5g / L penicillin solution, with the detection medium as the solvent) as the standard, and the results are as follows image 3 As shown, the results show that the concentration of penicillin in the first group is almost unchanged, while the degr...

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Abstract

The invention discloses rhodotorula mucilaginosa for degrading penicillin as well as application thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa was preserved in China Center for Type Culture Collection (CCTCC) on April 16, 2018, the preservation number is CCTCC M2018202, and the preservation address is Wuhan University, Wuhan, China. Compared with the existing bacterial strain with penicillin degrading capability, the rhodotorula mucilaginosa has the following advantages: the penicillin tolerance concentration (20 g/L) is 50 times that of the knownother bacterial strains in China; the penicillin concentration reducing value within the same time is far higher than the value which other bacterial strains can reach to; the rhodotorula mucilaginosais expected to be applied to treatment on bacterial residues in the actual production; and the capability of degrading the remained penicillin in the bacterial residues is extremely high.

Description

technical field [0001] The invention relates to a rhodotorula marcescens capable of degrading penicillin and an application thereof, which belongs to the technical field of microorganisms. Background technique [0002] Since penicillin was discovered in 1928, it has been widely used in clinical medicine because of its antibacterial properties, and it is still widely used today. Penicillin can hinder the synthesis of bacterial mucin, so that the cell wall cannot be synthesized smoothly, which in turn leads to the rupture and death of bacteria. During the fermentation process of some penicillin pharmaceutical factories, a large amount of solid waste residues will be produced, mainly containing microbial mycelia, organic matter that has not been metabolized, inorganic salts, a small amount of residual penicillin and its degradation products, and penicillin residues can be produced Organic fertilizers are used to increase the production of vegetables and grain crops, and are us...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23L5/20C05F11/00C05F17/00C12R1/645
CPCA23V2002/00A23L5/28C05F11/00C12N1/16C05F17/20C12N1/145C12R2001/645A23V2250/76Y02W30/40
Inventor 徐建中王礼君张伟国冯丽妍
Owner JIANGNAN UNIV
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