Method for preparing fermentation strains of microbial organic fertilizer

A technology for bio-organic fertilizer and fermentation strains is applied in the field of strain preparation for preparing tobacco straw bio-organic fertilizers by solid-state fermentation, and can solve the problems of no preferred selection of microbial strains, ineffective fermentation and transformation, poor effect, and the like. Yield and the effect of improving internal quality and increasing production value

Inactive Publication Date: 2011-04-27
中国烟草总公司湖北省公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, there is a method of fermenting tobacco stalks into bio-organic fertilizers by borrowing the solid fermentation technology of common crop stalks. However, because tobacco stalks contain a large amount of nicotine and contain a large number of harmful bacteria and viruses, this method does not preferably select microorganisms. species, the effect is not good
Commonly used fermentative bacteria are not suitable for survival under the condition of a large amount of nicotine substances, and cannot carry out effective fermentation transformation, so the existing methods are difficult to solve the above problems

Method used

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  • Method for preparing fermentation strains of microbial organic fertilizer
  • Method for preparing fermentation strains of microbial organic fertilizer

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Experimental program
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Effect test

Embodiment 1

[0032] Strain culture: take the above D 1 Bacteria tube 1 and S 4 1 bacterial tube, respectively inserted in 250ml seed bottles filled with culture medium, the culture medium consists of: 1% glucose, 0.8% peptone, 0.5% yeast extract, 0.4% sodium chloride, and the rest is water. Adjust the pH to 7.8.

[0033] Incubate the 250ml seed bottle for 20 hours at 28±1°C with shaking, and the D 1 Bacteria and S 4 After the bacteria solution is mixed in equal amounts, according to the seed amount of 6% volume ratio, it is then inserted into a 1000ml fermentation bottle; under 28±1°C and shaking conditions, the 1000ml fermentation bottle is cultivated for 24 hours, and the OD600nm value is measured to obtain a fermentation bacterial agent.

Embodiment 2

[0035] Strain culture: take the above D 1 3 bacterial tubes and S 4 1 bacterial tube, respectively inserted into 250ml seed bottles filled with culture medium, the composition of the culture medium is: 0.8% glucose, 0.6% peptone, 0.3% yeast extract, 0.2% sodium chloride, KH 2 PO 4 0.1%, the rest is water. Adjust the pH to 6.7.

[0036]Incubate 250ml seed bottles for 18 hours at 3°C ​​under shaking conditions, and then insert D into 1000ml fermentation bottles at 2% volume ratio. 1 Bacterial solution and 2.5% volume ratio seed quantity were inserted into S 4 Bacterial liquid: Under the condition of 33±℃ and shaking, cultivate 1000ml fermentation bottle for 18 hours, measure the OD600nm value, and obtain the fermentation bacterial agent.

Embodiment 3

[0038] Strain culture: take the above D 1 Bacteria tube 4 and S 4 1 bacterial tube, respectively inserted into 500ml seed bottles filled with culture medium, the composition of the culture medium is: 0.85% glucose, 0.7% peptone, 0.4% yeast extract, 0.3% sodium chloride, KH 2 PO 4 0.2%, the rest is water. Adjust the pH to 7.5.

[0039] Incubate 500ml seed bottles for 16 hours at 35°C under shaking conditions, and D 1 Bacteria and S 4 After the bacterial liquid is mixed, according to the 5% volume ratio of the seed amount, it is then connected to a 100L automatic fermenter for expanded cultivation. The pressure of the fermenter is controlled to 0.5mpa, the tank temperature is 2°C, the ventilation rate is 0.4 (v / v / min), and the stirring speed is 200rpm , cultured for 18 hours to obtain a fermentation broth.

[0040] The above-mentioned fermentation culture was concentrated 4 times by membrane filtration, and the liquid bacterial agent was detected.

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Abstract

The invention provides fermentation strains Brevibacterium sp D1 (with the perservation number of CCTCC No: M 2010250) and bacillus amyloliquefaciens S4 ( with the preservation number of CCTCC No: M 2010249) of a microbial organic fertilizer of tobacco straws; the fermentation strains Brevibacterium sp D1 and the bacillus amyloliquefaciens S4 are separated and screened from the tobacco straws under a high-temperature and heavy-nicotine condition, and can exist in the presence of the nicotine; and after being cultivated and proliferated, the ermentation strains Brevibacterium sp D1 and the bacillus amyloliquefaciens S4 are added into a waste tobacco straw mixture, and are subjected to solid fermentation under a natural condition to convert the tobacco straw wastes into the tobacco straw microbial organic fertilizer. The tobacco straw microbial organic fertilizer can replace other fertilizers to produce the tobacco, and has the advantages of increasing yield of the tobacco leaves, increasing production values of the tobacco leaves, improving smoking quality of the tobacco leaves, and simultaneously removing pollution to the environment by the tobacco straw wastes.

Description

technical field [0001] The invention relates to the production technology of agricultural bio-organic fertilizer, in particular to a method for preparing strains of tobacco stalk bio-organic fertilizer by solid-state fermentation. Background technique [0002] Tobacco is an important economic crop and plays a very important role in the national economy. In the process of tobacco planting and production in my country, chemical fertilizers have been used for a long time, resulting in a sharp decrease in the amount of soil organic matter, a decrease in the number and quality of aggregates, and degradation of air permeability, which affects the yield and quality of tobacco leaf production. In addition, after the tobacco leaves are harvested, a large amount of discarded tobacco stalks are discarded in the tobacco field, which can last up to five months, which will increase the pests and diseases of the tobacco field in the next year and pollute the ecological environment of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/13C12R1/07
Inventor 高勇陈家任杨树祖秉桥李锡宏
Owner 中国烟草总公司湖北省公司
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