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Construction method and applications of genetic engineering bacterium for producing L-arginine

A genetically engineered strain and genetic engineering technology are applied in the field of genetically engineered bacteria producing L-arginine, and can solve problems such as no reports of high-yielding recombinant bacteria yet.

Inactive Publication Date: 2014-08-06
SHANGHAI KING SHINE BIOLOGY TECH
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AI Technical Summary

Problems solved by technology

Although the use of genetic engineering to construct L-arginine high-yielding strains is a high-efficiency and rational breeding method, so far, there have been no reports of high-yielding recombinant bacteria.

Method used

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  • Construction method and applications of genetic engineering bacterium for producing L-arginine
  • Construction method and applications of genetic engineering bacterium for producing L-arginine
  • Construction method and applications of genetic engineering bacterium for producing L-arginine

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Embodiment Construction

[0023] 1. Materials and methods

[0024] 1.1 Strains and plasmids

[0025] Brevibacterium flavum ( Brevibacterium flavum ) belongs to Gram-positive bacteria and is a high-yield arginine mutant strain screened by our laboratory. The bacteria is an auxotrophic strain, hereinafter referred to as auxotrophic AN78 bacteria; Escherichia coli ) DH5α, BL21 (DE3) strains were preserved in our laboratory; Escherichia coli DH10B containing the shuttle vector pXMJ119 was purchased from Shaoxing Institute of Technology, Peking University; Corynebacterium glutamicum ATCC 13032 was purchased from Shanghai Fuxiang Biotechnology Co., Ltd. .

[0026] 1.2 Reagents and materials

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Abstract

The invention relates to a construction method and applications of a genetic engineering bacterium for producing L-arginine. The invention discloses a novel genetic engineering bacterium strain for producing L-arginine. Recombinant plasmid pXMJ19-argH can be orderly converted and introduced into colibacillus and auxotroph brevibacterium flavum AN78 by the genetic engineering bacterium strain, and then the argininosuccinase in the AN78 containing recombinant plasmids is over-expressed and fermented to produce L-arginine. The invention further discloses a construction method and applications of the genetic engineering bacterium. The genetic engineering bacterium can increase the L-arginine production output and reduce the production cost.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a genetically engineered bacterium for producing L-arginine, its construction method and its application. Background technique [0002] L-arginine (L-argnine, referred to as L-Arg) is a basic amino acid containing guanidine, an important amino acid in human metabolism, and an important intermediate metabolite in the urea cycle of organisms. L-arginine and its salts are also widely used in the treatment of various types of hepatic coma, in addition to being one of the main components of compound amino acid infusion. Sodium acid patients and patients with abnormal liver alanine aminotransferase have a significant effect on viral hepatitis. It has therapeutic effects on intestinal ulcers, thrombosis and neurasthenia. In addition, L-arginine is an important part of the formula of sports nutrition drinks, and also an important feed additive, which is widely used i...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/60C12N15/77C12P13/10C12R1/13
Inventor 王翠平马承国王开成张传军
Owner SHANGHAI KING SHINE BIOLOGY TECH
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