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Se-dopy ZM bacillus brevis and its uses in preparing biological red selenium

A short bacillus, biological technology, applied in the direction of bacteria, fermentation, etc.

Active Publication Date: 2007-07-11
张敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the utilization of selenium is greatly limited, and it is a research topic to develop high-efficiency and low-toxic selenium sources.

Method used

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  • Se-dopy ZM bacillus brevis and its uses in preparing biological red selenium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A selenophilic ZM brevibacterium, the selenophilic ZM brevibacterium is a brevibacterium of the genus Cytophaga (Cytophaga), the name of the preserved bacteria is: Sporocytophaga sp. General Microbiology Center of the Management Committee, deposit date: April 21, 2006, deposit number: CGMCC1694. The Brevibacterium selenophilus ZM has a cell length of 1-2 μm, a sliding movement mode, thin and shiny colonies, irregular edges, translucent, negative Gram's staining, and great differences in susceptibility to antibiotics.

[0028] The key to the success of gene therapy is the use of vectors to carry target genes and targeted high-efficiency expression. Screen out bacteria that have no toxic side effects on the human body, have a high affinity with selenium, and can use chemical energy as a carrier. Selenium binds to the bacteria to form selenoproteins, which are expressed in tumor cells in target organs to achieve specific killing of tumor tissues. .

[0029] The present i...

Embodiment 2

[0040] As described in Example 1, the application of Brevibacterium selenophilus ZM in the preparation of biological red selenium can be carried out in the following order:

[0041] (A) Bacterial liquid preparation:

[0042] Selenophilic ZM bacteria were inoculated in 1.5% peptone liquid medium, cultured at 37° C. for 24 hours, and set aside.

[0043] (B) preparation of biological red selenium:

[0044] Proceed in the following order:

[0045] 1) In 1000ml of 1-2% peptone liquid culture medium, add the above-mentioned bacterial solution and 10ml of 5% sodium selenite solution respectively, cultivate at 40°C, the fermentation broth changes from light yellow to red, and red can be seen under the microscope ZM bacteria sliding;

[0046] 2) After concentrated treatment, 50mg / ml (as Na 2 SeO 3 5H 2 O meter) red bacteria selenium.

[0047] The peptone liquid medium in the preparation of the above-mentioned item A bacteria liquid contains: 0.1% dipotassium hydrogen phosphate ...

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Abstract

The invention discloses an application of ZM brevibacterium to make biological umangite with reserving name as Sporocytophaga sp. and reserving data in the Apr. 21th 2006 and reserving number at CGMCC1694, which comprises the following steps: (A) inoculating ZM bacterium in the culture medium; (B) 1) adding bacterium liquid and sodium selenite in the culture medium; displaying the ferment liquid from light yellow into red to slide through microscope under visible red light; 2) condensing to obtain the product.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a selenophilic ZM brevibacterium and its application in the preparation of biological red selenium. Background technique [0002] Selenium was discovered and named by the Swedish chemist Bezeliu in 1817. In 1957, Schwarz and Foltz first proved that selenium is an essential trace element for animals and humans. In the 1980s, Chinese medical scientists first applied selenium supplementation to prevent and treat Keshan disease, and Kashin-Beck disease achieved remarkable results. A lot of work has confirmed that selenium has a wide range of biological effects, and selenium deficiency can cause a series of diseases: cancer, AIDS, cardiovascular disease, liver and pancreas disease, hypertension, diabetes, anemia, Keshan disease, etc. [0003] A series of biochemical effects of selenium are closely related to its concentration. The anticancer effect of selenium requires ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P3/00
Inventor 张敏刘晓涛
Owner 张敏
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